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  • 1
    Electronic Resource
    Electronic Resource
    Genetic analysis of morphological variability in three Plantago species with different mating systems (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 111-118 
    Details
    ISSN: 1432-2242
    Keywords: Morphological variability ; Plantago ; Differentiation ; Adaptive plasticity ; Mating system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Morphological variability in three Plantago species possessing different mating systems — P. Major, P. coronopus, and P. lanceolata — was analyzed. From each species several populations were analyzed. In comparing the species, it appeared that both the inbreeding P. major and the outbreeding P. lanceolata showed a high degree of morphological differentiation between populations. It was hypothesized that the absence of population differentiation for the species with a mixed mating system, P. coronopus, was caused by high adaptive plasticity. Plasticity is probably the predominant mechanism by which this species copes with different environments. There were considerable differences between populations within each species for their levels of variability and their heritabilities. It appeared that besides the influence of the mating system, selection may diminish morphological variability in the case of strong directional selection, especially in P. major and P. lanceolata.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226120
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Optimizing the generation of random amplified polymorphic DNAs in chrysanthemum (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 1033-1037 
    Details
    ISSN: 1432-2242
    Keywords: RAPDs ; Experimental design ; Fractional factorial ; Chrysanthemum ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Many conditions of the RAPD reaction procedure may influence the result. This paper presents rapid detection of influential factors with a fractional factorial experiment. A more extensive study of these factors is also presented. Polymerase brand, thermal cycler brand, annealing temperature, and primer, are important factors in obtaining good DNA yields and optimal fragment patterns. Each primer has its optimal annealing temperature, and this is not correlated with the GC content of the primer. Optimal species-primer combinations have to be found by trial and error.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00211058
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    RFLP analysis in chrysanthemum. I. Probe and primer development (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 472-478 
    Details
    ISSN: 1432-2242
    Keywords: RFLP ; PCR ; Chrysanthemum ; Genetic diversity ; Polyploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to study genetic variability at the DNA level in chrysanthemum (Dendranthema grandiflora Tzvelev) PstI and HindIII genomic libraries were constructed. Probes from both libraries were tested for the presence of restriction fragment length polymorphisms (RFLPs). Of the probes from the PstI library 91% appeared to hybridize to low-copy genes, while only 35% of those from the HindIII library appeared to do so. The PstI probes were used in further analyses as 79% of them showed RFLPs, whereas the HindIII low-copy number probes gave only 14% polymorphic patterns. Because of the hexaploid character of chrysanthemum, complex patterns generally consisting of 6–12 fragments were visible on a Southern blot after hybridization. To simplify the genetic analysis, locus-specific polymerase chain reaction (PCR) primers were developed that gave simple polymorphic patterns in a number of cases. The RFLP probes and primers developed will be used in future marker-assisted selection in this polyploid crop.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223663
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Identification of chrysanthemum cultivars and stability of DNA fingerprint patterns (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 439-447 
    Details
    ISSN: 1432-2242
    Keywords: Chrysanthemum ; RAPD ; DNA fingerprint ; RFLP ; Stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several techniques of DNA analysis were applied to identify chrysanthemum cultivars. Unrelated cultivars could be distinguished by using RAPDs (random amplified polymorphic DNAs), inter-SSR (simple sequence repeat) PCR (polymerase chain reaction), hybridization-based DNA fingerprinting, as well as RFLPs (restriction fragment length polymorphisms). Cultivars with different flower colours and belonging to one family, i.e. vegetatively derived from 1 cultivar, appeared to have the same DNA fragment patterns, whichever technique was applied. The absence of polymorphisms between different accessions of the same cultivar indicated a high stability of the observed patterns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222971
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    Paper (German National Licenses)
    Fulltext
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