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  • 1
    Electronic Resource
    Electronic Resource
    Detection of group C adenovirus DNA in small-cell lung cancer with the nested polymerase chain reaction (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 377-382 
    Details
    ISSN: 1432-1335
    Keywords: Adenovirus ; Small-cell lung cancer ; Polymerase chain reaction ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Group C adenovirus is latent in human tissues and can malignantly transform cells. The purpose of this study was to investigate the association between this virus and lung cancer. We investigated latent adenoviral infection using the nested polymerase chain reaction and in situ hybridization in transbronchial biopsy specimens from patients with small-cell lung cancer and non-small-cell lung cancer. The polymerase chain reaction was performed on DNA extracts with two sets of primers directed at a 261-base-pair target sequence of the E1A region of the adenoviral genome. In situ hybridization was performed on histological sections using DNA representing the entire adenovirus type 5 genome. E1A target DNA was present in 11 (31%) of 35 cases of small-cell lung cancer but in none of the 40 cases of non-small-cell lung cancer (P〈0.01). Of the 11 cases found positive by PCR, 8 were positive for adenovirus DNA by in situ hybridization. Adenovirus was prominent in tumor cells in 5 of the 8 cases, and in normal epithelial cells in the 3 remaining cases. Adenovirus DNA was not detected by in situ hybridization in specimens in which E1A DNA was not detected by the polymerase chain reaction. Small-cell lung cancer has mutations or deletions in the p53 and retinoblastoma genes more frequently than are found in non-small-cell lung cancer. Therefore, we speculate that adenovirus infection might participate in the pathogenesis of SCLC by producing mutation in these genes, rather than by inhibiting the function of these proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01240120
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  • 2
    Electronic Resource
    Electronic Resource
    Tuberculosis following liver transplantation: report of a case and review of the literature (1996)
    Springer
    Transplant international 9 (1996), S. 589-592 
    Details
    ISSN: 1432-2277
    Keywords: Tuberculosis, liver transplantation ; Liver transplantation, tuberculosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report on a 44-year-old man who developed tuberculosis 4 months after liver transplantation. The diagnosis was confirmed using a polymerase chain reaction (PCR) technique in bronchial alveolar lavage (BAL) fluid, and the patient was successfully treated by reducing his immunosuppression and administering antituberculous drugs. The patient became afebrile 20 days after starting antituberculous therapy and remains well at home. A review of the literature revealed that tuberculosis after liver transplantation is a rare complication with a reported mortality rate of as high as 40%. The mortality is highest for patients who become symptomatic within 3 months after transplantation (83% vs 0%, P〈0.01; Fisher's exact test) and for those with an interval between the initial symptom and diagnosis of more than 2 weeks (71% vs 0%, P〈0.05). Early diagnosis is, therefore, essential for successful resolution of tuberculosis after liver transplantation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335560
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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