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  • 1
    Electronic Resource
    Electronic Resource
    A deletion in the sapA homologue cluster is responsible for the loss of the S-layer in Campylobacter fetus strain TK (1997)
    Springer
    Archives of microbiology 167 (1997), S. 196-201 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsCampylobacter fetus ; sapA Homologues ; Promoter ; Chi sequence ; Antigenic variation ; Surface layer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The surface array protein (SAP) of Campylobacter fetus strain TK is encoded by seven homologous sapA genes clustered on the chromosomal DNA. The spontaneously arising variant strain TK(SAP–) produces no SAP and carries an approximately 10-kb chromosomal deletion. To elucidate the mechanism underlying the loss of SAP synthesis, we analyzed the region containing the sapA homologues and the deletion. We constructed a physical map of the sapA cluster region by aligning the clones that contain sapA homologues. These analyses demonstrated that all sapA homologues were located within a limited region of about 50 kb of chromosomal DNA of strain TK. The TK(SAP–) deletion was located within this cluster and was 13.3 kb in size. The deletion occurred between two sapA homologues and resulted in the formation of a chimeric sapA homologue in the variant strain. Sequence analysis of the upstream regions and the conserved regions of all sapA homologues revealed a high degree of similarity. However, only one sapA homologue contained a putative promoter sequence. This promoter sequence was located in the deleted region. Thus, the deletion of the promoter appears to be responsible for the loss of SAP expression in TK(SAP–).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050435
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  • 2
    Electronic Resource
    Electronic Resource
    Thymoma: Clinicopathologic features, therapy, and prognosis (1980)
    Springer
    Surgery today 10 (1980), S. 232-237 
    Details
    ISSN: 1436-2813
    Keywords: benign and malignant thymoma ; irradiation ; epithelial ; lymphocytic and lymphoepithelial cell types
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have treated 40 patients with thymoma. All the 18 with benign thymoma were treated with resection alone and none had a tumor recurrence or died from disease-related causes. Postoperative survival in this group ranged from 2 months to 14 years. Of the 22 patients with malignant thymoma, 2 underwent total resection, 10 partial resection and 10 were non-resectable. Fifteen of the 20 patients with non-resectable and partially excised thymomas were given radiotherapy. The cumulative 5- and 10-year survival rate of irradiation treated patients was 45.6%, and 34.4%, respectively. Of 6 non-irradiated patients, only one who underwent complete excision of tumors survived for more than 10 years, and 5 died within 3 years after treatment. Based on our findings we suggest that all patients with malignant thymoma, irrespective of the extent of surgical treatment, should be given postoperative irradiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02468753
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  • 3
    Electronic Resource
    Electronic Resource
    Abnormalities in maximum flow volume curve and closing volume in patients with hepatic cirrhosis (1980)
    Springer
    Surgery today 10 (1980), S. 265-269 
    Details
    ISSN: 1436-2813
    Keywords: hepatic cirrhosis ; maximum flow volume curve ; closing volume ; small airway ; interstitial pulmonary edema
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pulmonary functions were measured in 53 patients with hepatic cirrhosis in whom there was no clinical or radiographic evidence of pulmonary involvement. Spirometric tests such as total lung capacity, vital capacity, functional residual capacity, residual volume and forced expiratory volume during one second were within normal ranges, in all subjects. Flow volume curve and closing volume tests, however, were abnormal in the majority. The maximal expiratory flow at 50 per cent of vital capacity was not altered but the maximal expiratory flow at 25 percent of vital capacity was decreased significantly in patients with hepatic cirrhosis. The closing volume in patients with hepatic cirrhosis was also significantly increased. The abnormalities in flow volume curve and closing volume curve were also demonstrated in non-smokers, and at any age, in cases of hepatic cirrhosis. These results suggested that the narrowing or closure in small airways may occur in patients with hepatic cirrhosis. These changes may be due to mechanical compression of small airways by interstitial edema which was induced by presence in the circulating blood of vasoactive substances and endotoxins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02468786
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  • 4
    Electronic Resource
    Electronic Resource
    Phase II trial of combination chemotherapy with cisplatin, carboplatin and etoposide in stage IIIB and IV small-cell lung cancer (1998)
    Springer
    Cancer chemotherapy and pharmacology 41 (1998), S. 453-456 
    Details
    ISSN: 1432-0843
    Keywords: Key words Small-cell lung cancer ; Chemotherapy ; Cisplatin ; Carboplatin ; etoposide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Purpose: A phase II trial combining cisplatin, carboplatin and etoposide was conducted in previously untreated patients with stage IIIB and IV small-cell lung cancer, in an attempt to increase response rates and prolong survival. Methods: Previously untreated patients with small-cell lung cancer, with measurable disease, aged ≤ 72 years, performance status ≤ 2, and adequate hematologic, hepatic and renal function were enrolled in the study. They were treated with 80 mg/m2 cisplatin on day 1, 100 mg/m2 carboplatin on days 2, 3 and 8, and 50 mg/m2 etoposide on days 1, 2, 3 and 8. Results: A total of 46 patients (20 with stage IIIB and 26 with stage IV disease) were enrolled in the study. A total of 186 courses of chemotherapy were given, and the dose was reduced in 27 courses (15%). The chemotherapy was repeated for four or more courses in 30 patients. There were 10 complete responses and 32 partial responses, for a total response rate of 91% (95% confidence interval, 79% to 98%). The median survival time and 2-year survival rates were 18 months and 22% for stage IIIB disease, and 14 months and 15% for stage IV disease. Major side effects were hematologic: leukopenia, anemia, and thrombocytopenia of grade 3 or more occurred in 48%, 46%, and 43% of patients, respectively. Conclusions: The three-drug regimen of cisplatin, carboplatin and etoposide is feasible and active against small-cell lung cancer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002800050766
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  • 5
    Electronic Resource
    Electronic Resource
    Granulocyte-colony stimulating factor promotes invasion by human lung cancer cell lines in vitro (1996)
    Springer
    Clinical & experimental metastasis 14 (1996), S. 351-357 
    Details
    ISSN: 1573-7276
    Keywords: granulocyte-colony stimulating factor ; invasion ; invasion assay ; lung cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: The effects of exogenous and endogenous granulocyte colony-stimulating factor (G-CSF) on invasion by cancer cells were studied, using lung cancer cell lines that produce G-CSF (NCI-H157) and lines that do not (PC-9 and NCI-H23). The invasive capacity of NCI-H157 cells was 26- to 27-fold higher than that of PC-9 and NCI-H23 cells. The invasiveness of PC-9 cells was stimulated by exogenous G-CSF, while that of NCI-H157 cells was not. Antibodies against G-CSF blocked the stimulation of PC-9 cell invasiveness by exogenous G-CSF. Anti G-CSF antibodies also inhibited invasion by NCI-H157 cells in the absence of exogenous G-CSF. These results suggest that endogenous and exogenous G-CSF both stimulate invasion by lung cancer cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00123394
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  • 6
    Electronic Resource
    Electronic Resource
    G-CSF increases secretion of urokinase-typeplasminogen activator by human lung cancer cells (1998)
    Springer
    Clinical & experimental metastasis 16 (1998), S. 551-558 
    Details
    ISSN: 1573-7276
    Keywords: G-CSF ; invasion ; lung cancer ; uPA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We reported previously that granulocyte colony-stimulating factor (G-CSF) can promote the invasion ofhuman lung cancer cell lines in vitro. However, the exact mechanism of its stimulatory effect on invasionremains to be elucidated. In the present study we mainly focused our attention on the components of theplasminogen activation system in human lung cancer cell lines, because of the central role that plasminogenactivators play in regulating extracellular proteolysis. We showed that G-CSF induced a dose-dependentincrease in the urokinase-type plasminogen activator (uPA) activity in the conditioned medium of a PC-9lung cancer cell line. When the amounts of uPA activity were quantitated by densitometry, we found thateven at a concentration of 0.01 mg/ml, G-CSF had a stimulatory effect on the uPA release, while highconcentrations caused a 3.6-fold increase at a maximum concentration of 1 mg/ml. A Western blot analysisof the conditioned medium confirmed the findings observed in a zymographic analysis. The observed increasein uPA protein was paralleled by a significant increase in the uPA mRNA levels after treatment withG-CSF. However, our experiments failed to identify any alteration in the plasminogen activator inhibitor(PAI) secretion caused by G-CSF. In addition, we also found the expression of G-CSF receptor by PC-9cells, suggesting the possible pathway activated by G-CSF.©Kluwer Academic Publishers
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006546402703
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  • 7
    Electronic Resource
    Electronic Resource
    Detection of group C adenovirus DNA in small-cell lung cancer with the nested polymerase chain reaction (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 377-382 
    Details
    ISSN: 1432-1335
    Keywords: Adenovirus ; Small-cell lung cancer ; Polymerase chain reaction ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Group C adenovirus is latent in human tissues and can malignantly transform cells. The purpose of this study was to investigate the association between this virus and lung cancer. We investigated latent adenoviral infection using the nested polymerase chain reaction and in situ hybridization in transbronchial biopsy specimens from patients with small-cell lung cancer and non-small-cell lung cancer. The polymerase chain reaction was performed on DNA extracts with two sets of primers directed at a 261-base-pair target sequence of the E1A region of the adenoviral genome. In situ hybridization was performed on histological sections using DNA representing the entire adenovirus type 5 genome. E1A target DNA was present in 11 (31%) of 35 cases of small-cell lung cancer but in none of the 40 cases of non-small-cell lung cancer (P〈0.01). Of the 11 cases found positive by PCR, 8 were positive for adenovirus DNA by in situ hybridization. Adenovirus was prominent in tumor cells in 5 of the 8 cases, and in normal epithelial cells in the 3 remaining cases. Adenovirus DNA was not detected by in situ hybridization in specimens in which E1A DNA was not detected by the polymerase chain reaction. Small-cell lung cancer has mutations or deletions in the p53 and retinoblastoma genes more frequently than are found in non-small-cell lung cancer. Therefore, we speculate that adenovirus infection might participate in the pathogenesis of SCLC by producing mutation in these genes, rather than by inhibiting the function of these proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01240120
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  • 8
    Electronic Resource
    Electronic Resource
    Detection of group C adenovirus DNA in small-cell lung cancer with the nested polymerase chain reaction (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 377-382 
    Details
    ISSN: 1432-1335
    Keywords: Key words Adenovirus ; Small-cell lung cancer ; Polymerase chain reaction ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Group C adenovirus is latent in human tissues and can malignantly transform cells. The purpose of this study was to investigate the association between this virus and lung cancer. We investigated latent adenoviral infection using the nested polymerase chain reaction and in situ hybridization in transbronchial biopsy specimens from patients with small-cell lung cancer and non-small-cell lung cancer. The polymerase chain reaction was performed on DNA extracts with two sets of primers directed at a 261-base-pair target sequence of the E1A region of the adenoviral genome. In situ hybridization was performed on histological sections using DNA representing the entire adenovirus type 5 genome. E1A target DNA was present in 11 (31%) of 35 cases of small-cell lung cancer but in none of the 40 cases of non-small-cell lung cancer (P〈0.01). Of the 11 cases found positive by PCR, 8 were positive for adenovirus DNA by in situ hybridization. Adenovirus was prominent in tumor cells in 5 of the 8 cases, and in normal epithelial cells in the 3 remaining cases. Adenovirus DNA was not detected by in situ hybridization in specimens in which E1A DNA was not detected by the polymerase chain reaction. Small-cell lung cancer has mutations or deletions in the p53 and retinoblastoma genes more frequently than are found in non-small-cell lung cancer. Therefore, we speculate that adenovirus infection might participate in the pathogenesis of SCLC by producing mutation in these genes, rather than by inhibiting the function of these proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01240120
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  • 9
    Electronic Resource
    Electronic Resource
    Tuberculosis following liver transplantation: report of a case and review of the literature (1996)
    Springer
    Transplant international 9 (1996), S. 589-592 
    Details
    ISSN: 1432-2277
    Keywords: Tuberculosis, liver transplantation ; Liver transplantation, tuberculosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report on a 44-year-old man who developed tuberculosis 4 months after liver transplantation. The diagnosis was confirmed using a polymerase chain reaction (PCR) technique in bronchial alveolar lavage (BAL) fluid, and the patient was successfully treated by reducing his immunosuppression and administering antituberculous drugs. The patient became afebrile 20 days after starting antituberculous therapy and remains well at home. A review of the literature revealed that tuberculosis after liver transplantation is a rare complication with a reported mortality rate of as high as 40%. The mortality is highest for patients who become symptomatic within 3 months after transplantation (83% vs 0%, P〈0.01; Fisher's exact test) and for those with an interval between the initial symptom and diagnosis of more than 2 weeks (71% vs 0%, P〈0.05). Early diagnosis is, therefore, essential for successful resolution of tuberculosis after liver transplantation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335560
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  • 10
    Electronic Resource
    Electronic Resource
    Case report 812 (1993)
    Springer
    Skeletal radiology 22 (1993), S. 549-551 
    Details
    ISSN: 1432-2161
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209109
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