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  • Adrenalectomy  (2)
  • Neurosecretory material  (2)
  • Anti-oxytocinase serum  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Influence of adrenalectomy on “Gomori-positive” substances in the hypothalamo-neurohypophysial system of rats heterozygous and homozygous for hypothalamic diabetes insipidus (1977)
    Springer
    Anatomy and embryology 151 (1977), S. 81-89 
    Details
    ISSN: 1432-0568
    Keywords: Neurosecretion ; Adrenalectomy ; Diabetes insipidus ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The influence of adrenalectomy on the amount of “Gomori-positive” neurosecretory material in the outer layer of the median eminence and in the supraoptico-hypophysial system was studied in normal Long-Evans rats and in Long-Evans rats heterozygous and homozygous for hypothalamic diabetes insipidus. In all non-adrenalectomized rats very few “Gomori-positive” granules were found in the outer median eminence layer. After adrenalectomy the amount of the granules increased markedly in normal Long-Evans rats and only slightly in Long-Evans rats heterozygous for diabetes insipidus. However, no augmentation of the granules occurred in homozygous diabetes insipidus rats. The amount of “Gomori-positive” substances demonstrable in the supraoptic and paraventricular nuclei, in the inner layer of the median eminence, and in the neural lobe of the hypophysis was less in rats heterozygous for diabetes insipidus than in normal Long-Evans rats and the smallest in homozygous diabetes insipidus rats. No differences in the amounts of the substances were observed between adrenalectomized and non-adrenalectomized animals. The findings suggest that the “Gomori-positive” granules occurring in the outer layer of the median eminence of adrenalectomized rats are of similar origin as those of the supraoptico-hypophysial system and represent a vasopressin-neurophysin-complex. The results support the concept that vasopressin is involved in the regulation of ACTH release.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315300
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Immunohistochemical demonstration of a CRF-associated neurophysin in the external zone of the rat median eminence (1974)
    Springer
    Cell & tissue research 152 (1974), S. 411-421 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence (rat) ; External zone ; Adrenalectomy ; CRF-neurophysin ; Immunoperoxidase histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In picric acid-formalin fixed, paraplast embedded median eminence sections from normal and bilaterally adrenalectomized rats, the amount of “Gomori-positive” neurosecretory material and the amount of protein, cross reacting against a porcine neurophysin-II antiserum were determined. In normal rats, “Gomori-positive” substances and neurophysin were confined to the internal zone of the median eminence. After adrenalectomy, however, “Gomori-positive” granules and a neurophysin-like protein also became apparent in the external zone. Their amount increases, if the animals are treated with DOCA from the 15th to the 21st day after the operation. The distribution of the neurophysin-like protein in the external zone is similar to that of the “Gomori-positive” granules. Since the granules are regarded as a morphological correlate of the corticotropin-releasing factor (CRF) it is assumed, that the protein, appearing in the external zone after adrenalectomy and cross-reacting against the neurophysin antiserum is a CRF-associated neurophysin. For this substance the name “CRF-neurophysin” is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218928
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Neurosecretion in the external and internal zone of the median eminence of the cat and dog (1974)
    Springer
    Cell & tissue research 155 (1974), S. 201-210 
    Details
    ISSN: 1432-0878
    Keywords: External median eminence (Cat, dog) ; Neurosecretory material ; CRF ; Neurophysin ; Immunoperoxidase histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The immunoglobulin-enzyme bridge technique, in association with rabbit antiporcine neurophysin-II has been applied to the median eminence of the dog and cat in order to study the distribution of neurophysin-like antigens throughout this area of the brain and correlate the findings with the corresponding distribution of neurosecretory material (NSM) as revealed by the crotonaldehyde fuchsin stain. Neurophysin and NSM were both present in the hypothalamo-supraoptico-neurohypophysial system—the pathway taken by oxytocin, vasopressin and neurophysin from the hypothalamus to the posterior pituitary lobe. Whereas the tuberoinfundibular tract of the median eminence was almost devoid of NSM, the presence of neurophysin-like material was clearly evident using immunoperoxidase histochemistry. The significance of a protein in the external median eminence possessing determinants cross-reactive against anti-neurophysin serum is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221354
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Time of fixation and the localization of gomori-positive and neurophysin-containing structures in the rat hypothalamus (1975)
    Springer
    Cell & tissue research 162 (1975), S. 523-530 
    Details
    ISSN: 1432-0878
    Keywords: Neurophysin ; Neurosecretory material ; Magnocellular nuclei (rat) ; Postmortem fixation ; Polyacrylamide gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rats were decapitated and the complete head stored at 4°C. At various time intervals, up to a maximum of 14 days, the brains and pituitary glands were removed and fixed in formalin-picric acid. Neurosecretory material (NSM), as revealed with crotonaldehyde fuchsin, and neurophysin as demonstrated by means of immunoperoxidase histochemistry were localized in the hypothalamic magnocellular nuclei, median eminence and posterior pituitary gland. NSM and neurophysin were present in the paraventricular (PVN) and supraoptic (SON) nuclei up to periods of 14 days although the cellular morphology was disrupted after about 4 days. After 4 days there was an abundance of positive staining “droplets” in both the SON, PVN and along the nerve fibres of the supraoptico-neurohypophysial tract. Pituitary gland NSM and neurophysin were still present 14 days post mortem. The slab gradient polyacrylamide gel electrophoretic pattern of soluble proteins extracted from the posterior pituitary gland from the experimental and normal animals showed almost complete identity confirming the minimal action of proteolytic enzymes at 4°C up to a period of 6 days. It is concluded that as long as the tissue is cooled, rapid fixation of hypothalamic tissue is not essential for the localization of NSM and neurophysin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209351
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Oxytocinase-immunohistochemical demonstration in the immature and term human placenta (1975)
    Springer
    Cell & tissue research 162 (1975), S. 531-539 
    Details
    ISSN: 1432-0878
    Keywords: Human placenta ; Syncytiotrophoblast ; Oxytocinase ; Anti-oxytocinase serum ; Immunofluorescence histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Oxytocinase (cystine aminopeptidase) was purified from human retroplacental serum by a combination of fractional precipitation, hydroxylapatite chromatography and gel exlusion chromatography on Sephadex G-200. The purified enzyme possessed a specific activity of 980 mIU/mg using L-cystine-di-p-nitroanilide as substrate. This represented a 3200 fold concentration from the starting material in an overall yield of 12%. Antibodies against oxytocinase were raised in rabbits and the γ-globulin fraction labelled with fluorescein isothiocyanate prior to its use in the immunofluorescence histochemical localization of the enzyme in human placental tissue. Oxytocinase was confined to the syncytiotrophoblastic cells of normal term, and immature placentas as well as in placentas from patients suffering from severe toxaemia. Specific immunofluorescence was also present in the outer margins of the chorion and to a lesser extent in the amnion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209352
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    Paper (German National Licenses)
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