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  • Neurosecretory pathways  (3)
  • Adrenalectomy  (2)
  • Anti-oxytocinase serum  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Influence of adrenalectomy on “Gomori-positive” substances in the hypothalamo-neurohypophysial system of rats heterozygous and homozygous for hypothalamic diabetes insipidus (1977)
    Springer
    Anatomy and embryology 151 (1977), S. 81-89 
    Details
    ISSN: 1432-0568
    Keywords: Neurosecretion ; Adrenalectomy ; Diabetes insipidus ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The influence of adrenalectomy on the amount of “Gomori-positive” neurosecretory material in the outer layer of the median eminence and in the supraoptico-hypophysial system was studied in normal Long-Evans rats and in Long-Evans rats heterozygous and homozygous for hypothalamic diabetes insipidus. In all non-adrenalectomized rats very few “Gomori-positive” granules were found in the outer median eminence layer. After adrenalectomy the amount of the granules increased markedly in normal Long-Evans rats and only slightly in Long-Evans rats heterozygous for diabetes insipidus. However, no augmentation of the granules occurred in homozygous diabetes insipidus rats. The amount of “Gomori-positive” substances demonstrable in the supraoptic and paraventricular nuclei, in the inner layer of the median eminence, and in the neural lobe of the hypophysis was less in rats heterozygous for diabetes insipidus than in normal Long-Evans rats and the smallest in homozygous diabetes insipidus rats. No differences in the amounts of the substances were observed between adrenalectomized and non-adrenalectomized animals. The findings suggest that the “Gomori-positive” granules occurring in the outer layer of the median eminence of adrenalectomized rats are of similar origin as those of the supraoptico-hypophysial system and represent a vasopressin-neurophysin-complex. The results support the concept that vasopressin is involved in the regulation of ACTH release.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315300
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Localization of neurophysin in the neurosecretory elements of the hypothalamus and neurohypophysis of the normal and osmotically stimulated guinea-pig as demonstrated by immunofluorescence histochemical techniques (1973)
    Springer
    Cell & tissue research 145 (1973), S. 39-55 
    Details
    ISSN: 1432-0878
    Keywords: Magnocellular hypothalamic nuclei ; Accessory structures ; Neurosecretory pathways ; Neurophysin-like material ; Immunofluorescence histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Antisera to porcine neurophysin-II and ovine neurophysin-III were used to localize neurophysin-like material in the hypothalamus and neurohypophysis of guinea-pigs with an immunofluorescence technique. Although the guinea-pig appears to have only one major neurophysin it was found to be localized in both of the bilateral magnocellular nuclei. Neurophysin-like material was also present in extreme rostral portions of the hypothalamus, in cells lying between the third ventricle and the supraoptic nucleus and in a cluster of cells dorsomedial to the fornix. Immunofluorescence was observed in neurosecretory fibres that followed pathways previously characterized with classical histological stains for “neurosecretory material”. The immunofluorescence in the hypothalamic elements of a normal guinea-pig was not greatly different from that in fluorescent structures present in an animal that had been severely dehydrated. In contrast, there was a marked depletion of neurophysin from the posterior pituitary gland of the dehydrated guinea-pig. The lack of graded fluorescence in the hypothalamus of the dehydrated animal is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307188
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Neurophysin and the neurosecretory fibres of the sheep infundibulum (1973)
    Springer
    Cell & tissue research 145 (1973), S. 471-478 
    Details
    ISSN: 1432-0878
    Keywords: Neurophysins (sheep) ; Neurosecretory pathways ; Proximal and distal neurohypophysis ; Infundibulum ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A cross-species reactive antiserum was used to study the cellular localization of neurophysin in the sheep hypothalamus and neurohypophysis. The immunofluorescent technique was used to follow the neurosecretory fibres emanating from the magnocellular nuclei to the lower infundibular stem. It was confirmed that neurophysin was present in both the internal and external zone of the infundibulum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306719
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Immunohistochemical demonstration of a CRF-associated neurophysin in the external zone of the rat median eminence (1974)
    Springer
    Cell & tissue research 152 (1974), S. 411-421 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence (rat) ; External zone ; Adrenalectomy ; CRF-neurophysin ; Immunoperoxidase histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In picric acid-formalin fixed, paraplast embedded median eminence sections from normal and bilaterally adrenalectomized rats, the amount of “Gomori-positive” neurosecretory material and the amount of protein, cross reacting against a porcine neurophysin-II antiserum were determined. In normal rats, “Gomori-positive” substances and neurophysin were confined to the internal zone of the median eminence. After adrenalectomy, however, “Gomori-positive” granules and a neurophysin-like protein also became apparent in the external zone. Their amount increases, if the animals are treated with DOCA from the 15th to the 21st day after the operation. The distribution of the neurophysin-like protein in the external zone is similar to that of the “Gomori-positive” granules. Since the granules are regarded as a morphological correlate of the corticotropin-releasing factor (CRF) it is assumed, that the protein, appearing in the external zone after adrenalectomy and cross-reacting against the neurophysin antiserum is a CRF-associated neurophysin. For this substance the name “CRF-neurophysin” is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218928
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Oxytocinase-immunohistochemical demonstration in the immature and term human placenta (1975)
    Springer
    Cell & tissue research 162 (1975), S. 531-539 
    Details
    ISSN: 1432-0878
    Keywords: Human placenta ; Syncytiotrophoblast ; Oxytocinase ; Anti-oxytocinase serum ; Immunofluorescence histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Oxytocinase (cystine aminopeptidase) was purified from human retroplacental serum by a combination of fractional precipitation, hydroxylapatite chromatography and gel exlusion chromatography on Sephadex G-200. The purified enzyme possessed a specific activity of 980 mIU/mg using L-cystine-di-p-nitroanilide as substrate. This represented a 3200 fold concentration from the starting material in an overall yield of 12%. Antibodies against oxytocinase were raised in rabbits and the γ-globulin fraction labelled with fluorescein isothiocyanate prior to its use in the immunofluorescence histochemical localization of the enzyme in human placental tissue. Oxytocinase was confined to the syncytiotrophoblastic cells of normal term, and immature placentas as well as in placentas from patients suffering from severe toxaemia. Specific immunofluorescence was also present in the outer margins of the chorion and to a lesser extent in the amnion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209352
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Immunocytochemical study of the hypothalamo-neurohypophysial system (1977)
    Springer
    Cell & tissue research 180 (1977), S. 467-490 
    Details
    ISSN: 1432-0878
    Keywords: Oxytocin ; Vasopressin ; Neurophysin ; Magnocellular nuclei ; Neurosecretory pathways ; Osmotic stimulation ; Immunoperoxidase histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Antisera, with cross reactive antibodies removed by affinity chromatography, were used in the immunoperoxidase-bridge technique to study the distribution of oxytocin and vasopressin together with neurophysin in the hypothalamo-neurohypophysial system of the rat. The hormones were demonstrated in different areas of the supraoptic nucleus (SON) and paraventricular nucleus (PVN), in neurosecretory fibres of the hypothalamoneurohypophysial tract, median eminence, and in nerve terminals of the neurohypophysis. Intact normal and rats with hereditary hypothalamic diabetes insipidus (Brattleboro strain), and rats dehydrated by the administration of oral hypertonic saline were studied. In dehydrated rats the hormone concentration in the neurons, and the number of neurons containing hormone varied according to the time of dehydration stress. The observations support the hypotheses that: 1) oxytocin and oxytocinneurophysin, and vasopressin and vasopressin-neurophysin are synthesised in different neurons and are transported along different axons; 2) the SON and PVN are functionally indistinguishable in that neurons containing oxytocin or vasopressin are present in both nuclei; and 3) the two types of neurons respond to osmotic stimulation in a way that is qualitatively the same but quantitatively different.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220169
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    Paper (German National Licenses)
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