ISSN:
1432-1912
Keywords:
Carbohydrate Metabolism
;
Kidney
;
Enzymic Determination of Substrates
;
6-Aminonicotinamide
;
Adrenalectomy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary After application of 6-aminonicotinamide (6-AN), a strong accumulation of 6-phosphogluconate was found in rat kidney. The accumulation of 6-phosphogluconate influences the activity of the phosphoglucose isomerase. The enzymic determination of the substrate concentrations of the carbohydrate metabolism after application of 6-AN revealed that fructose-6-phosphate and lactate as well as phosphoenolpyruvate are significantly increased in the kidney as compared to the controls. The increase in phosphoenolpyruvate might be caused by stimulated gluconeogenesis, as the application of 6-AN increases the release of corticosterone from the adrenal cortex. After adrenalectomy, the accumulation of 6-phosphogluconate is decreased, the ratio glucose-6-phosphate/fructose-6-phosphate is normalized, and also the values for lactate and phosphoenolpyruvate decrease. The effects of adrenaline seem to involve the passage of glucose through the pentose phosphate pathway. There is probably a relationship between the blockade of the oxidative pentose phosphate pathway and the stimulation of gluconeogenesis in the kidney, the mechanism of which is unknown. In contrast to the findings in the brain, no changes could be established in the concentrations of citrate and 2-oxoglutarate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01002174
Permalink
