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  • 1
    Electronic Resource
    Electronic Resource
    Perfusion technique of suckling rat liver, and comparison of cytologic and biochemical properties between hepatocytes isolated from suckling and adult rats (1986)
    Springer
    Research in experimental medicine 186 (1986), S. 121-131 
    Details
    ISSN: 1433-8580
    Keywords: Suckling rat hepatocytes ; Adult rat hepatocytes ; Collagenaseliver-perfusion technique ; Primary culture ; Liver-specific functions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Collagenase-liver-perfusion technique, currently used with adult rat liver, was applied to isolation of hepatocytes from suckling rat liver. The total hepatocyte numbers isolated from suckling rats by collagenase-liverperfusion technique were 9-fold higher than those by non-perfusion technique. The yield and viability of isolated hepatocytes from suckling rats were 18.1 × 107 cells per gram liver and 95%, respectively. The cell yield per gram liver and viability from suckling rats were 185% and 112% of those from adult ones, respectively. In comparison with adult rat hepatocytes, suckling rat hepatocytes were smaller and more homogeneous. The percentage (3.1%) of binucleate cells in the hepatocytes isolated from suckling rats was about one tenth of that (30.7%) in the hepatocytes isolated from adult rats. The isolated suckling rat hepatocytes had higher tyrosine aminotransferase (TAT) and glucose-6-phosphate (G6Pase) activities than adult ones. Suckling and adult rat hepatocytes were transferred to primary culture to compare their cell number kinetics and functional longevities. The functional longevities of those hepatocytes were assessed by their capacity to secrete albumin and alpha-fetoprotein (AFP) into the culture medium and to express TAT and G6Pase activities up to day 6 of primary culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851993
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation of clones from T24 cells and test of their sensitivity to adriamycin (1988)
    Springer
    Research in experimental medicine 188 (1988), S. 239-246 
    Details
    ISSN: 1433-8580
    Keywords: T24 ; Heterogeneity ; Single cell clone ; Adriamycin ; Chemosensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two single cell clones, CL3 and CL7, were developed from T24 cells. CL3 had abundant cytoplasmic granules, while CL7 did not have as much. CL3 and CL7 exhibited a modal number of chromosomes at 69 and 74, respectively. CL7 had a higher growth rate than CL3, as indicated by in vitro cell growth, plating efficiency, and growth of the tumors produced in nude mice. CL3 was slightly susceptible to adriamycin toxicity as compared to CL7.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01852272
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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