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  • 1
    Electronic Resource
    Electronic Resource
    Promotion of flowering and morphological alterations in Atropa belladonna transformed with a CaMV 35S-rolC chimeric gene of the Ri plasmid (1992)
    Springer
    Plant cell reports 12 (1992), S. 1-6 
    Details
    ISSN: 1432-203X
    Keywords: Atropa belladonna ; Flowering ; Ri plasmid ; rolC gene ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacterium mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35S promoter, the rolC gene was expressed strongly in leaves, flowers, stems and roots. The transformed plants exhibited dramatic promotion of flowering, reduced apical dominance, pale and lanceolated leaves and smaller flowers. On the other hand, when native rolC gene was co-introduced with NPT II, the transgenic plants obtained did not exhibit the altered phenotypes observed in 35S-rolC transformants, and the expression level of the rolC gene was much lower than in 35S-rolC transformants. These results suggest that the morphological changes in transgenic Atropa belladonna were related to the degree of expression of the rolC gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232412
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  • 2
    Electronic Resource
    Electronic Resource
    Action spectrum for light-induced formation of adventitious shoots in hairy roots of horseradish (1993)
    Springer
    Planta 189 (1993), S. 590-592 
    Details
    ISSN: 1432-2048
    Keywords: Action spectrum ; Adventitious bud formation ; Armoracia ; Hairy roots ; Light and bud formation ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An action spectrum was determined for lightinduced formation of adventitious shoots in hairy roots of horseradish (Armoracia rusticana Gaert., Mey. et Scherb.) cultured in vitro. Near ultraviolet (350–400 nm), blue (440–460 nm) and red light (600–680 nm) were most effective in inducing formation of adventitious buds. Farred light (730 nm) inhibited the promotive effect of all three wavelength regions. These results are consistent with induction by phytochrome(s) of adventitious shoots in hairy roots of horseradish.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198224
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of light and growth regulators on adventitious bud formation in horseradish (Armoracia rusticana) (1995)
    Springer
    Plant cell reports 14 (1995), S. 611-615 
    Details
    ISSN: 1432-203X
    Keywords: Adventitious bud formation ; Horseradish (Armoracia rusticana) ; Leaf ; Light ; Phytohormone ; Root
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To clarify that the presence of Ri T-DNA genes are not prerequisite for the light-induced bud formation in horseradish (Armoracia rusticana) hairy roots, leaf and root segments of nontransformed horseradish plants were used as explants. Bud formation from nontransformed tissues was observed in hormone-free medium under 16 h daylight conditions, but not under continuous darkness. To investigate the effects of growth regulators on bud formation, leaf and root explants were treated with auxin (1-naphthaleneacetic acid; NAA) and / or cytokinin (6-benzyl-aminopurine; BA). The most effective treatment in the dark to stimulate bud formation was BA at 1 mg·1-1. These results show that adventitious bud formation in horseradish can be induced by light and growth regulators, and especially cytokinin, may be involved in bud formation, irrespective of whether the tissues were transformed with Ri T-DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232723
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation and characterization of a cDNA that encodes ECP31, an embryogenic-cell protein from carrot (1992)
    Springer
    Plant molecular biology 19 (1992), S. 239-249 
    Details
    ISSN: 1573-5028
    Keywords: ABA ; Daucus carota ; ECP31 ; gene expression ; LEA clone ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A full-length cDNA for ECP31, an embryogenic cell protein from carrot (Daucus carota L.) with a M r of 31000 (Kiyosue T, Satoh S, Kamada H, Harada H (1991) Plant Physiol 95: 1077–1083), was isolated from a cDNA library prepared from embryogenic cells using PCR-amplified DNA as a probe. The genomic Southern blot analysis revealed that there are two or three genes for ECP31 in the carrot genome. The transcripts of ECP31 accumulated in the peripheral regions of clusters of embryogenic cells and disappeared in the course of somatic embryogenesis that was induced by transfer of the embryogenic cells to auxin-free media. The cDNA encodes a polypeptide of 256 amino acids, and the calculated molecular weight of this polypeptide is 26 111. The deduced amino acid sequence shows a high degree (62.2%) of similarity to that of a protein that is abundant during late embryogenesis of cotton (LEA D34; Baker JC, Steele C, Dure III (1988) Plant Mol Biol 11: 227–291). The mRNAs for ECP31 started to accumulate in zygotic embryos at a late stage of embryogenesis but were undetectable in mature embryos within 24 h after imbibition of seeds. In dry fruits (seeds), the transcripts were detected only in zygotic embryos by in situ hybridization. The level of ECP31 transcripts increased after treatment with abscisic acid (ABA) in torpedo-shaped somatic embryos but not in seven-day-old seedlings. These results suggest that both embryo-specific factor(s) and ABA are involved in the expression of the gene for ECP31.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027345
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    Paper (German National Licenses)
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