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  • Aegilops squarrosa Wheat  (1)
  • Amplification  (1)
  • Introgression  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Translocations and other karyotypic structural changes in wheat x rye hybrids regenerated from tissue culture (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 547-554 
    Details
    ISSN: 1432-2242
    Keywords: Wheat X rye hybrids ; Translocations ; Introgression ; Tissue culture ; C-banding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The spontaneous occurrence of chromosome breaks, deletions, and translocations in plant tissue cultures is well documented. This study investigated the usefulness of tissue culture as a method of introgressing alien genes into wheat. Wheat X rye hybrids were regenerated from embryo scutellar calli maintained in culture for 222 days. The regenerated seedlings then were treated with colchicine to produce amphidiploids (AABBDDRR). The karyotypes of ten amphidiploids were analyzed by C-banding to determine chromosome structural changes that occurred during tissue culture. Three wheat/rye and one wheat/wheat chromosome translocations, seven deletions, and five amplifications of heterochromatin bands of rye chromosomes were identified. One amphidiploid contained a reciprocal translocation between wheat chromosome 4D and rye chromosome 1R. Non-reciprocal translocations between 2B and 3R, and between an unidentified wheat chromosome and 2R, were found independently in two amphidiploids. An additional plant had a translocation between wheat chromosomes 6B and 5A. All deletions involving rye chromosomes were noted in all 10 amphidiploids. Twelve of the 13 breakpoints in chromosomes involved in translocations and deletions occurred in heterochromatin. Amplification of heterochromatin bands on 2RL and 7RL chromosome arms also was observed in five plants. These results indicate a high degree of chromosome structural change induced by tissue culture. Therefore, tissue culture may be a useful tool in alien gene introgression and manipulation of heterochromatin in triticale improvement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285012
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Use of winter wheat x Triticum tauschii backcross populations for germplasm evaluation (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 571-577 
    Details
    ISSN: 1432-2242
    Keywords: Triticum tauschii ; Aegilops squarrosa Wheat ; Yield ; Protein ; Hardness ; Leaf rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The wild diploid goatgrass, Triticum tauschii (Coss.) Schmal., is an important source of genes for resistance to both diseases and insects in common wheat (Triticum aestivum L.) We have evaluated grain yield, kernel weight, protein concentration, and kernel hardness of 641 BC2 F1-derived families from direct crosses involving four T. aestivum cultivars and 13 T. tauschii accessions over 2 years and at two Kansas, USA, locations. On average, T. tauschii germplasm depressed grain yield and increased protein concentration, whereas kernel weight was affected either positively or negatively, depending on the T. tauschii parent. Three T. tauschii parents produced a large proportion of families with very soft endosperm. Some variation among progeny of different T. tauschii parents resulted from the segregation of genes for resistance to leaf rust (caused by Puccinia recondita Rob. ex Desm.). This study confirmed that random BC2-derived families can be used to evaluate the effects of T. tauschii genes in the field. This methodology, although laborious, can provide useful information which is not obtainable by the screening of T. tauschii accessions themselves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222005
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Amplification of repeated DNA sequences in wheat X rye hybrids regenerated from tissue culture (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 381-388 
    Details
    ISSN: 1432-2242
    Keywords: Amplification ; Repeated DNA sequences ; WheatX rye hybrids ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Previous C-banding analysis of wheat (Triticum aestivum)X rye (Secale cereale) hybrids regenerated from tissue culture revealed enlarged C-bands in some rye chromosomes, but the molecular nature of the change was not determined. In situ hybridization using two DNA probes containing repeated sequences from rye telomeric heterochromatin was conducted on these wheatX rye hybrids and their progeny to investigate the occurrence of amplification in repeated sequences. Clones pSC 74 and pSC 119, which contain sequences from the 480-bp and 120-bp repeated DNA families of rye, respectively, were used as probes. Amplification of 480-bp repeated sequences in the short arm telomere of chromosome 7R was detected in three wheatxrye hybrids and their progeny. The amplified 480-bp sequences were detected by an enlarged hybridization site for pSC 74 at the 7RS telomere, and by the appearance at this same telomeric site of an unlabeled, blue chromosome segment in an otherwise completely brown chromosome hybridizing entirely to the biotin-labeled pSC 119 probe. This variant form of chromosome 7R was not observed in several ‘Chaupon’ plants, or in the other hybrids derived from the same embryos, indicating the origin of the change in tissue culture. The amplified sequences were inherited up to at least three generations. Deletions and translocations were also observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276739
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    Paper (German National Licenses)
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