ISSN:
1432-2242
Keywords:
Aerial yam
;
Dioscorea bulbifera L.
;
Chloroplast DNA
;
Physical map
;
Clone bank
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A physical map of chloroplast DNA (ctDNA) of aerial yam, Dioscorea bulbifera L. was constructed using three restriction endonucleases, PstI, SalI, and SmaI. In addition, a clone bank of the BamHI-digested fragments were generated, and the locations of most BamHI fragments on the map were also determined. The ctDNA of D. bulbifera was found to be a circular molecule with a total size of ca. 152 kb involving two inverted repeats of ca. 25.5 kb, and small and large single copy regions of ca. 18.5 and 83.4 kb, respectively. The genes for the large subunit of the ribulose 1,5-bisphosphate carboxylase (rbcL) and the ATP-synthase subunits β and ɛ (atpB/atpE) were mapped.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00299745
Permalink
