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  • 1
    ISSN: 1432-0983
    Keywords: Key words aflR ; Aflatoxin ; Secondary metabolism ; Zinc binuclear cluster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Under limiting growth conditions, Aspergillus nidulans produces a carcinogenic secondary metabolite related to aflatoxin and called sterigmatocystin (ST). The genes for ST biosynthesis are co-ordinately regulated and are all found within an approximately 60-kilobase segment of DNA. One of the genes within this region is predicted to encode a CX2CX6CX6CX2CX6CX2 zinc binuclear cluster DNA-binding protein that is related to the Aspergillus flavus and Aspergillus parasiticus aflatoxin regulatory gene aflR. Deletion of the A. nidulans aflR homolog resulted in an inability to induce expression of genes within the ST gene cluster and a loss of ST production. Because A. nidulans aflR mRNA accumulates specifically under conditions that favor ST production we expect that activation of ST biosynthetic genes is determined by A. nidulans aflR. In support of this hypothesis, we demonstrated that induced expression of the A. flavus aflR gene in A. nidulans, under conditions that normally suppress ST gene expression, resulted in activation of genes in the ST biosynthetic pathway. This result demonstrates that AflR function is conserved between Aspergillus spp. and that aflR expression is sufficient to activate genes in the ST pathway.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1439-0973
    Keywords: Key Words Aborigines ; Hepatitis A ; Hepatitis B ; Hepatitis C ; Taiwan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Background: Taiwan is endemic for viral hepatitis infections. A field survey was performed in the isolated aborigines in Hualien, eastern Taiwan, to investigate the geographic and ethnic variations in hepatitis epidemiology. Materials and Methods: From 1996 to 1998, blood was drawn from 1,748 subjects from two southern Ami and two northern Atayal villages for serum markers of hepatitis A, B and C. Results: Hepatitis A infection approached 100% in all groups. Hepatitis B infection and carrier rates were higher in the Atayal than in the Ami (92.3% vs 49.1% and 20.8% vs 5.3%; p 〈 0.01). Hepatitis C infection rates were higher in three villages (27.5%, 20.1% and 25.4% vs 3.6%; p 〈 0.01). Hepatitis C infection increased with age (p 〈 0.01) while hepatitis B infection did not. Conclusion: Hepatitis A infected most aborigines before the age of 15 years. Hepatitis B seldom infected people after the age of 15 years, while hepatitis C continued to infect people who were older. Geographic factors are important for hepatitis C infection, whereas for hepatitis B infection, in addition ethnicity is also important.
    Type of Medium: Electronic Resource
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