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  • After-cooking blackening  (1)
  • Calcium influx  (1)
  • Cell volume  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The invariance of macromolecular composition with altered light limited growth rate of Amphidinium carteri (dinophyceae) (1985)
    Springer
    Archives of microbiology 142 (1985), S. 81-86 
    Details
    ISSN: 1432-072X
    Keywords: Carbon-fixation ; Cell volume ; Dinophyceae ; Growth rate ; Irradiance ; Macromolecular composition ; Nucleic acid composition ; Amphidinium carteri
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of irradiance on the growth rate, macromolecular composition and photosynthetic carbon metabolism of Amphidinium carteri was studied in batch culture. Growth rate increased linearly with increasing irradiance up to a maximum growth rate of 0.04 h-1 at an irradiance of 80 μEm-2s-1. In contrast to a number of other studies on both prokaryotic and eukaryotic microorganisms, ours showed that cellular content of RNA, DNA, protein and carbohydrate of A. carteri were invariant with growth rate over the range μ=0.04 to 0.007 h-1. The invariant macromolecular composition was correlated with a constant modal cell volume. Chlorophyll and lipid per cell increased with decreasing irradiance. The distribution of [14C]-bicarbonate in the major end products of photosynthesis after incubation with isotope for 14% of a doubling time showed that the percentage carbon in the chloroform (lipids and pigments) fraction increased with decreasing irradiance while that of the trichloroacetic acid soluble (carbohydrate) fractions decreased. The percentage of isotope in the trichloroacetic acid insoluble (protein) fraction and methanol: water fraction (metabolites) remained constant. Because this species, under light-limited growth, differs from other organisms so far studied, more species must be similarly examined before nucleic acid content is used as an index growth rate in the field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409242
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Prevention of after-cooking darkening of irradiated potatoes (1977)
    Springer
    Potato research 20 (1977), S. 77-84 
    Details
    ISSN: 1871-4528
    Keywords: After-cooking blackening ; after-cooking darkening ; gamma rays ; sprout inhibition ; phenolics ; carotenoids ; irradiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potatoes of the variety ‘Kufri Chandramukhi’ exposed to 10 krad of gamma rays for sprout inhibition, tended to develop after-cooking darkening, after storage at 15°C, for over three months. The darkening could be prevented or reduced (a) by pre-peeling of tubers prior to cooking: or (b) by reconditioning the potatoes by keeping them at 34–35°C for 4–6 days; or (c) by soaking and cooking the tubers in solutions of diaminoethane-tetra-acetic acid disodium salt (EDTA) or citric acid. Prepeeled tubers cooked in the presence of the peel tissues or added phenolics like caffeic and chlorogenic acids showed darkening. The results implicate that this could be due to the interaction of phenolics with Fe++ present in the tissues. The storage of irradiated tubers at low temperature also showed enhanced disapperance of carotenoids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02362302
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    An influx of extracelluar calcium is required for initiation of the human sperm acrosome reaction induced by human follicular fluid (1988)
    New York, NY : Wiley-Blackwell
    Gamete Research 20 (1988), S. 397-411 
    Details
    ISSN: 0148-7280
    Keywords: Calcium influx ; G-75 fraction ; fura-2 ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The role of Ca2+ in the human sperm acrosome reaction was investigated using the fluorescent calcium indicator fura-2. Previous experiments have shown that a Sephadex G-75 column fraction of human follicular fluid can stimulate the human sperm acrosome reaction [Suarez SS, Wolf DP, Meizel S (1986): Gamete Res 14:107-121]. Using fura-2, we demonstrated that this Sephadex G-75 fraction also stimulates a rapid, transient increase in intracellular free Ca2+. This Ca2+ transient is blocked either by chelation of extracellular calcium or by addition of the Ca2+ antagonist La3+. We have also been able to stimulate the acrosome reaction in human sperm without significant loss of motility, using the divalent cation ionophore ionomycin. Acrosome reactions stimulated by whole follicular fluid, the G-75 fraction, or ionomycin are all blocked by removal of extracellular Ca2+. These results strongly suggest that an influx of extracellular Ca2+ is responsible for intiating the acrosome reaction in human sperm treated with human follicular fluid. This is the first demonstration in mammalian sperm that a potentially physiological stimulus can cause an increase in intracellular Ca2+ concomitant with the acrosome reaction.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120200402
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    Paper (German National Licenses)
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