ISSN:
1432-2048
Keywords:
Agrobacterium
;
Brassica (transformed plants)
;
Genetic manipulation
;
Osmotic pressure
;
Potassium
;
Solanum (transformed plants)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Growth, water content, osmotic pressure and solute content were examined for normal potato (Solanum tuberosum L. cv. Desiree) and a derivative (line D9X8a), which was genetically transformed with TL-DNA from Agrobacterium rhizogenes. Plants were grown (i) in vitro, (ii) in a growth chamber and (iii) in the field. In vitro, the transformed potato plants produced more biomass than the untransformed plants, partly because they had a higher water content. Potassium concentration and osmotic pressure were lower in cell sap extracted from the transformed potato shoots. In some cases the difference was as much as 50%. These differences were less clear, absent or reversed in plants from a growth chamber or from the field. In the field, however, transformed potato senesced early. It is suggested that a cellular basis for these observations may be changes induced by Ri TL-DNA expression products in plant membrane properties.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00407016
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