ISSN:
1432-203X
Keywords:
Agrobacterium
;
mediated transformation
;
Pepino
;
pKIWI110
;
Regeneration
;
Solanum muricatum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Regeneration of pepino (Solanum muricatum Ait.) shoots was achieved both by organogenesis and by embryogenesis. Shoots derived via organogenesis were easily rooted and most regenerated plants appeared phenotypically normal. Transgenic plants were obtained using the binary vector pKIWI110 in the avirulent Agrobacterium tumefaciens strain LBA4404. Optimization of transformation protocols was rapidly achieved by monitoring early expression of the GUS (β-D-glucuronidase) reporter gene carried on pKIWI110. Transgenic plants expressed GUS and selectable marker genes for kanamycin resistance and chlorsulfuron resistance. PCR (polymerase chain reaction) and Southern analysis provided molecular evidence for transformation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00234297
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