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Ultrastructural morphometric characterization of alveolar type II cells of perinatal sheep: a comparison with adults (1992)

Lakritz, Jeffrey ; Weir, Alison J. ; Mariassy, Andrej T. ; [et al.]

Springer

Anatomy and embryology 186 (1992), S. 355-362

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ISSN: 1432-0568

Keywords: Perinatal ; Neonatal ; Adult ; Type II cell ; Sheep lung development

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary The quantitative morphologic changes in alveolar type II cells during the perinatal period were characterized morphometrically in the lungs of fetal lambs at 132, 138, and 147 days gestational age (DGA) and in newborns at 2 days postnatal age (2 DPN). Ultrastructural features were compared with those of type II cells of ewes 365 days old. Lamellar body profile number per type II cell profile was highest at term (147 DGA) and 2 DPN. In adults, the number of lamellar body profiles and volume density of lamellar bodies were equal to those of the 132 DGA fetus. Multivesicular bodies were most common at 138 DGA and in adults. The volume density of cytoplasmic glycogen fell dramatically during the latter part of gestation. The volume density of many cellular organelles increased to the level observed in adults by term (147 DGA). Subcellular composition of type II cells of adult sheep differs from that reported for adult rats chiefly by the volume density of lamellar material within the cytoplasm. Plate-like or globe-like inclusions were present only in the type II cells of adults. Cytoplasmic extensions of the type II cell crossing the basal lamina were most abundant in the 132 and 138 DGA fetal sheep. Cytoplasmic extensions were rare in adults. We conclude that morphologic changes of the alveolar type II cell associated with gestational age follow a species-specific time course. In the sheep, this occurs during the later part of gestation and extends into the neonatal period. Morphologic and morphometric changes appear to correspond with cellular interactions between alveolar type II cells and mesenchymal cells of the interstitium.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00185985

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Aerosolized fluorescent microspheres detected in the lung using confocal scanning laser microscopy (1993)

Pinkerton, Kent E. ; Gallen, John T. ; Mercer, Robert R. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Microscopy Research and Technique 26 (1993), S. 437-443

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ISSN: 1059-910X

Keywords: Particle deposition ; Nebulization ; Albumin-coated microspheres ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Natural Sciences in General

Notes: Aerosolized fluorescent microspheres were used to study particle deposition in site-specific regions of the lung with confocal laser scanning microscopy. A nebulizer was used to aerosolize microspheres followed by passage through a heated discharging column to reduce static charge and to remove water surrounding each microsphere. Precoating of microspheres with albumin helped to minimize displacement during vascular fixation of the lungs. Confocal laser microscopy facilitated visualization of microspheres throughout the bronchial tree, ducts, and alveoli of the lungs. The use of fluorescent microspheres and confocal laser imaging provided distinct advantages compared with other methods to study lung particle deposition due to (1) the generation of single microspheres of uniform size by nebulization, (2) easy detection of microspheres in large slabs of microdissected lung tissues, (3) excellent resolution of tissue surfaces and microspheres for an infinite number of orientations and planes of section, and (4) the ability to visualize microspheres below fluid lining layers and on surfaces that could not easily be done by other methods of microscopy.© 1993 Wiley-Liss, Inc.

Additional Material: 5 Ill.