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  • Alkaline phosphatase  (6)
  • Enzyme induction  (4)
  • Flow cytometry  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Synergistic induction of alkaline phosphatase in colonic carcinoma cells by sodium butyrate and hyperosmolality
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 718 (1982), S. 220-223 
    Details
    ISSN: 0304-4165
    Keywords: (Colon carcinoma cell) ; Alkaline phosphatase ; Enzyme induction ; Hyperosmolality ; Sodium butyrate
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0304-4165(82)90222-7
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  • 2
    Electronic Resource
    Electronic Resource
    Divergent effects of butyrate on the alkaline phosphatases of SW-620 cells
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Molecular Basis of Disease 1180 (1993), S. 289-293 
    Details
    ISSN: 0925-4439
    Keywords: (Cultured colon cancer cell) ; Alkaline phosphatase ; Butyrate ; Enzyme induction
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0925-4439(93)90052-3
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  • 3
    Electronic Resource
    Electronic Resource
    Preferential alkaline phosphatase isoenzyme induction by sodium butyrate
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Molecular Cell Research 762 (1983), S. 289-294 
    Details
    ISSN: 0167-4889
    Keywords: (SW-620 cell) ; Alkaline phosphatase isozyme ; Enzyme induction ; Sodium butyrate
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4889(83)90083-6
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Modulation of alkaline phosphatases in LoVo, a human colon carcinoma cell line
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Molecular Cell Research 1013 (1989), S. 259-265 
    Details
    ISSN: 0167-4889
    Keywords: (Cultured colon cancer cell) ; Alkaline phosphatase ; Enzyme induction ; Hyperosmolality ; Sodium butyrate
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0167-4889(89)90144-4
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of human serum on alkaline phosphatase induction in cultured human tumor cells (1989)
    Springer
    Cellular and molecular life sciences 45 (1989), S. 753-755 
    Details
    ISSN: 1420-9071
    Keywords: Alkaline phosphatase ; human serum ; cultured tumor cells ; enzyme induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The continuous cell lines T 24 and HT-29, derived from human bladder and colon carcinomas, produce term-placental and intestinal alkaline phosphatase, respectively. Growth in hyperosmolar medium or exposure to prednisolone or sodium butyrate induces increased enzyme levels, and combinations of inducers elicit synergistic activity increases. The effect of the inducing agents is strikingly diminished when cells are grown in the presence, of high concentrations of human serum, and the synergistic increases are essentially abolished. Major human serum protein fractions do not affect alkaline phosphatase induction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01974578
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  • 6
    Electronic Resource
    Electronic Resource
    Alkaline phosphatase activity in cultured meningioma cells (1982)
    Springer
    Acta neuropathologica 57 (1982), S. 45-50 
    Details
    ISSN: 1432-0533
    Keywords: Meningioma ; Alkaline phosphatase ; Cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The specific activity of alkaline phosphatase in cultured human meningioma cells varies over a relatively wide range. There is no correlation between the levels of activity and the histological type of meningioma from which the cultures were derived. The enzyme is heat-labile and is strongly inhibited byl-homoarginine, levamisole, and l-bromotetramisole, but unaffected byl-phenylalanine andl-phenylalanylglycylglycine. These findings indicate that meningioma cells synthesize the liver/bone/kidney form of alkaline phosphatase. In contrast to cultures derived from pituitary adenomas, glioblastomas, and astrocytomas in which prednisolone and/or sodium butyrate elicit a manifold increase of alkaline phosphatase activity, with meningioma cells the hormone causes only a slight augmentation in specific activity, and the fatty acid is ineffective. As with other cells producing the liver/bone/kidney enzyme form, no increase in activity occurs in meningioma cells growing in hyperosmolar medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688876
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  • 7
    Electronic Resource
    Electronic Resource
    Alkaline phosphatase isozymes in cultured human cancer cells (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1357-1361 
    Details
    ISSN: 1420-9071
    Keywords: Alkaline phosphatase ; isozymes ; tumor cell lines ; cultured cells ; HeLa cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Alkaline phosphatase, an ubiquitous enzyme is known to exist in several isozymic forms. At least three different isozymes have now been identified in humans. Alkaline phosphatase isozymes are among the substances synthesized ectopically by a variety of human tumors and many continuous cell lines derived from different cancers have retained the capacity to produce these membrane-located glycoproteins. This paper reviews the identification of alkaline phosphatase isozymes in cultured tumor cells and relates these finding with recent developments concerning these cell membrane located glycoproteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01949989
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  • 8
    Electronic Resource
    Electronic Resource
    Flow cytometric analysis of the DNA distribution in human brain tumors (1979)
    Springer
    Acta neuropathologica 46 (1979), S. 39-44 
    Details
    ISSN: 1432-0533
    Keywords: Flow cytometry ; DNA distribution ; Brain tumor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Flow cytofluorometric analysis was used to determine the distribution of the DNA content in cells from selected areas of normal human brain and in benign and malignant brain tumors. Propidium iodide was employed as DNA fluorochrome and the analysis was carried out on a suspension of single cells. Normal, nonstimulated human lymphocytes were used as diploid controls. With nonneoplastic tissue an average of 91% of the cells were diploid (presumably in G0 or G1 stage of the cell cycle). The cells of most benign tumors were mainly diploid (77–98%), nine specimens of pituitary adenomas had large numbers of aneuploid cells. In glioblastoma multiforme the proportion of diploid cells was significantly diminished and polyploid cells were frequently seen. Similar results were obtained in other malignant tumors, with metastatic tumors showing the greatest ploidy variation, which included triploid, tetraploid, and hypertetraploid cells. The analytical method used provides valuable information of significant clinical importance on the DNA distribution in brain tumor cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684802
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  • 9
    Electronic Resource
    Electronic Resource
    Flow-through cytometry of meningiomas and cultured meningioma cells (1978)
    Springer
    Acta neuropathologica 44 (1978), S. 183-187 
    Details
    ISSN: 1432-0533
    Keywords: Meningioma ; Flow cytometry ; Cell size ; DNA ; Cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Flow cytometric techniques were used to compare the DNA content, size and viability of meningioma cells obtained directly from surgical specimens with the same cells after a period of culture. Cells isolated from the original meningiomas and cells in primary culture were similar with regard to size and DNA content, regardless of the histologic subclassification of tumor. The cell populations were essentially diploid with a small proportion of tetraploid cells. Viable cells were smaller and more uniform in size than the nonviable cells. An increase in the number of cells having an elevated DNA content was seen with cultures repeatedly transferred. The latter results suggest that any transfer of information from long-term cultured meningioma cells to the in vivo situation must be done with caution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691065
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