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  • 1
    Electronic Resource
    Electronic Resource
    Flow-through cytometry of meningiomas and cultured meningioma cells (1978)
    Springer
    Acta neuropathologica 44 (1978), S. 183-187 
    Details
    ISSN: 1432-0533
    Keywords: Meningioma ; Flow cytometry ; Cell size ; DNA ; Cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Flow cytometric techniques were used to compare the DNA content, size and viability of meningioma cells obtained directly from surgical specimens with the same cells after a period of culture. Cells isolated from the original meningiomas and cells in primary culture were similar with regard to size and DNA content, regardless of the histologic subclassification of tumor. The cell populations were essentially diploid with a small proportion of tetraploid cells. Viable cells were smaller and more uniform in size than the nonviable cells. An increase in the number of cells having an elevated DNA content was seen with cultures repeatedly transferred. The latter results suggest that any transfer of information from long-term cultured meningioma cells to the in vivo situation must be done with caution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691065
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    An ultrastructural study of cultured human meningioma cells (1979)
    Springer
    Acta neuropathologica 46 (1979), S. 11-15 
    Details
    ISSN: 1432-0533
    Keywords: Meningioma ; Cell culture ; Ultrastructure ; Cell junctions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ultrastructural alterations in human meningioma cells grown in vitro are reported. In early passages the cells retain some of the characteristic features of the original tumors. These include interdigitations associated with intercellular junctional devices (e.g., desmosomes, gap junctions). However, with repeated subculture these features tend to be less frequent. Typical whorl formations are observed only in primary cultures. The nunber of cytoplasmic filaments, lipid inclusions and other dense bodies increases with time in culture. Cytoplasmic invaginations into nuclei and the appearance of very large cells become more frequent in repeatedly transferred cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684798
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Flow cytometric analysis of the DNA distribution in human brain tumors (1979)
    Springer
    Acta neuropathologica 46 (1979), S. 39-44 
    Details
    ISSN: 1432-0533
    Keywords: Flow cytometry ; DNA distribution ; Brain tumor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Flow cytofluorometric analysis was used to determine the distribution of the DNA content in cells from selected areas of normal human brain and in benign and malignant brain tumors. Propidium iodide was employed as DNA fluorochrome and the analysis was carried out on a suspension of single cells. Normal, nonstimulated human lymphocytes were used as diploid controls. With nonneoplastic tissue an average of 91% of the cells were diploid (presumably in G0 or G1 stage of the cell cycle). The cells of most benign tumors were mainly diploid (77–98%), nine specimens of pituitary adenomas had large numbers of aneuploid cells. In glioblastoma multiforme the proportion of diploid cells was significantly diminished and polyploid cells were frequently seen. Similar results were obtained in other malignant tumors, with metastatic tumors showing the greatest ploidy variation, which included triploid, tetraploid, and hypertetraploid cells. The analytical method used provides valuable information of significant clinical importance on the DNA distribution in brain tumor cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684802
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of sugar-beet pulp on ruminal and lactation performances of cows having different rumen fermentation patterns
    Amsterdam : Elsevier
    Animal Feed Science and Technology 31 (1990), S. 91-104 
    Details
    ISSN: 0377-8401
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0377-8401(90)90116-P
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Effects of whole cottonseed on ruminal properties and lactation performance of cows with different rumen fermentation patterns
    Amsterdam : Elsevier
    Animal Feed Science and Technology 34 (1991), S. 203-212 
    Details
    ISSN: 0377-8401
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0377-8401(91)90111-5
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Expression of neurite outgrowth factor and gicerin during inner ear development and hair cell regeneration in the chick (1997)
    Springer
    Journal of neurocytology 26 (1997), S. 501-509 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Several cell adhesion molecules are expressed in the developing inner ear. The present study focused on gicerin, a novel member of the immunoglobulin superfamily, in an attempt to improve our understanding of the development and regeneration of chick inner ear. Gicerin is known to homophilically interact with itself and to bind to neurite outgrowth factor (NOF). The data collected herein show that gicerin is highly expressed in auditory epithelium and acoustic ganglion during early embryogenesis. The immunoreactivity of gicerin in the auditory epithelium decreases more rapidly than that in the acoustic ganglion as the mature hair cells become distinguishable. At the post-hatch stage, the expression of gicerin is not observed. In contrast, NOF was expressed on the basement membranes around the auditory epithelium, and in the acoustic ganglion during development and after birth, but not in the auditory epithelium. Following noise damage, gicerin is transiently re-expressed on the damage receptor epithelium when active cell proliferation is observed in the epithelium. This positive reaction immediately disappears as immature short hair cells appear. These results suggest that gicerin may be associated with cell proliferation in the auditory epithelium, and play a role in neurite extension of the acoustic ganglion cells in conjunction with NOF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018585508713
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    Paper (German National Licenses)
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