Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Alkaloids  (1)
  • Key words: Mast cells — Antigen-mediated stimulation — Myosin I  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Loss of cell surface microvilli on rat basophilic leukaemia cells precedes secretion and can be mimicked using the calmodulin antagonist trifluoperazine (1997)
    Springer
    Inflammation research 46 (1997), S. 354-360 
    Details
    ISSN: 1420-908X
    Keywords: Key words: Mast cells — Antigen-mediated stimulation — Myosin I
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Objective: We studied changes in cell surface morphology following treatment with secretagogue or trifluoperazine in a mast cell model.¶Materials and Methods: Rat basophilic leukaemia (RBL) cells were treated with antigen and or the calmodulin antagonist, 0–50 μM trifluoperazine (TFP). The release of a secretory granule enzyme, β-hexosaminidase, into the external medium was used as a measure of secretion. Quantitation of cell surface microvilli was determined by using a computer with input from a digitising tablet from scanning electron micrographs. Cytoskeletal proteins present in microvilli were analysed by confocal immunofluorescence.¶Results: When RBL cells are stimulated to secrete with an antigen, the cell surface is transformed from a microvillous morphology to a ruffled one. The cell surface rearrangement preceded β-hexosaminidase secretion: the majority of microvilli disappeared rapidly after stimulation (t1/2 of 39 s) whereas secretion can only be measured after a lag of 47 s. The calmodulin antagonist, TFP did not inhibit antigen-induced secretion or loss of microvilli, however TFP alone caused a similar loss of microvilli but was unable to stimulate or potentiate secretion. The microvilli mostly disappeared within 30 s, and a half-maximal effect occurred at approximately 8 μM TFP. Using immunofluorescence, calmodulin was localized to punctate structures on the dorsal cell surface which presumably correspond to the microvilli, and which also stained for F-actin and myosin I.¶Conclusions: Loss of cell surface microvilli on RBL cells precedes secretion and could reflect a cytoskeletal rearrangement which facilitates fusion of secretory granules with the membrane. It can be mimicked using trifluoperazine and we suggest it may involve calmodulin-binding components of the microvillus cytoskeleton such as myosin I.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000110050201
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Relationship of manganese with iron and zinc with respect to latex yield and composition in opium poppy (Papaver somniferum Linn) under two fertility conditions (1993)
    Springer
    Nutrient cycling in agroecosystems 34 (1993), S. 55-58 
    Details
    ISSN: 1573-0867
    Keywords: Alkaloids ; fertility ; iron ; manganese ; Papaver sominferum L. ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two different field experiments were conducted for two years during 1985–86 and 1986–87 to study the relationship of Mn (0, 15 and 30 kg ha−1) with Fe (0, 15 and 30 kg ha−1) and Zn (0, 10 and 20 kg ha−1) under two fertility (NPK) conditions on yield and quality of opium poppy. The main effect of these micronutrients with respect to latex yield was highest at 15 kg ha−1 of Mn or Fe and 10 kg ha−1 Zn. Increasing the level beyond that resulted in reduction in latex yield. Highest response was observed when 15 kg ha−1 Mn was applied with either 15 kg ha−1 Fe or 10 kg ha−1 Zn. Morphine, codeine, narcotine and thebaine content of the latex was highest with 15 kg Mn, 15 kg Fe or 10 kg ha−1 Zn or the combined application of 15 kg Mn with 15 kg Fe or 10 kg Zn ha−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00749960
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...