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  • 1
    ISSN: 1432-2242
    Keywords: Allium fistulosum ; Repeated DNA sequence ; In situ hybridization ; Nucleotide sequence ; C-banding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A highly repeated DNA sequence with a repeating unit of approximately 380bp was found in EcoRV digests of the total genomic DNA of Allium fistulosum. Three independent clones containing this unit were isolated, and their repeating units sequenced. These units showed more than 94% sequence homology, and the copy number was estimated to be about 2.8×106 per haploid genome. In situ hybridization, with the repeating unit as a probe, and C-banding analyses indicated that the repeated DNA sequence of A. fistulosum is closely associated with the major C-heterochromatin in the terminal regions of all 16 chromosomes at mitotic metaphase. The characters of the repeating unit are similar to those of the A. cepa unit, which is taxonomically closely related to A. fistulosum.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 57 (1980), S. 33-35 
    ISSN: 1432-2242
    Keywords: Flow activation energy ; Membrane fluidity ; Protoplast fusion ; Rauwolfia serpentina var. ‘Bentham’ ; Temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The fusion of plant protoplasts is greatly enhanced by a rise in temperature in the presence of the fusion-inducing agents polyethylene glycol or polyvinyl alcohol. Kinetic analysis of this reaction reveals that membrane fluidity is important in regulating the fusion process.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Fluorescent chromosome banding patterns ; Phaseolus ; Vigna ; Phylogenetic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phylogenetic relationships among eight taxa of seven species of Phaseolus and Vigna (Phaseolus angularis, P. aureus, P. calcaratus, P. coccineus, P. vulgaris, Vigna sesquipedalis and V. sinensis; 2n = 22 each) were studied by the fluorescent chromosome banding technique. Preparations of somatic metaphase chromosomes of each taxon were sequentially stained with Giemsa, GC-specific fluorochrome chromomycin A3 (CMA) and AT-specific fluorochrome 4′-6-diamidino-2-phenylindole (DAPI). On the basis of the fluorescent banding patterns of the 22 chromosomes of each taxon, P. angularis, P. coccineus (from China and Korea) and P. vulgaris were grouped into one group (“Phaseolus group”), P. aureus and two Vigna species were grouped into another (“Vigna group”) and P. calcaratus was grouped in an independent group.
    Type of Medium: Electronic Resource
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