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  • Atrazine resistance  (2)
  • protoplast fusion  (2)
  • Alternaria  (1)
  • Alternaria brassicicola  (1)
  • B. napus  (1)
  • Bipolaris  (1)
Source
Material
Years
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Studies on the fungal phytotoxin victorin: Structures of three novel amino acids from the acid hydrolyzate (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1370-1374 
    Details
    ISSN: 1420-9071
    Keywords: Cochliobolus victoriae ; Helminthosporium ; Bipolaris ; Avena
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The host-selective phytotoxin victorin, produced by the fungusCochliobolus victoriae, was found to be at least partially peptidic in nature, and did not contain victoxinine. The exact mass of the M-H ion was measured by FABMS as 795.1877. Derivatives of three major acid hydrolysis products were isolated. The structures of the corresponding amino acids were assigned as 2S,3R-3-hydroxyleucine, 5,5-dichloroleucine, and 3-hydroxylysine. A into victorin by the fungus in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01949994
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Regeneration of plants from protoplasts of Capsella bursa-pastoris and somatic hybridization with rapid cycling Brassica oleracea (1999)
    Springer
    Plant cell reports 18 (1999), S. 412-417 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsCapsella bursa-pastoris ; Rapid-cycling Brassica oleracea ; Protoplasts ; Intertribal somatic hybrids ; Alternaria brassicicola
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fertile rooted plantlets were recovered from leaf mesophyll protoplasts of Capsella bursa-pastoris. Protoplasts cultured over a feeder layer of Brassica napus cells produced 221 colonies, 7 of which regenerated multiple plantlets. The nuclear DNA content of most regenerates varied from 0.89 to 1.0 pg/nucleus, close to the value for seed-grown C. bursa-pastoris (0.94±0.03 pg/nucleus). Two regenerants had a tetraploid DNA content (1.8– 2.0 pg). Plants with a DNA content close to Capsella produced seeds, both in vitro and in soil. Intertribal somatic hybrids were obtained by polyethylene glycol-mediated fusion of untreated C. bursa-pastoris protoplasts with iodoacetate-treated protoplasts of rapid-cycling B. oleracea. Plants were confirmed as somatic hybrids by isozyme and RAPD analysis. The nuclear DNA content of the hybrids ranged from 3.2 to 6.4 pg, higher than the sum of the parental genomes. One of two hybrids tested was resistant to Alternaria brassicicola, like the Capsella fusion partner. Hybrids rooted easily and produced sterile flowers when transplanted to soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050595
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Improved resistance to bacterial soft rot by protoplast fusion between Brassica rapa and B. oleracea (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 810-819 
    Details
    ISSN: 1432-2242
    Keywords: Key words Bacterial soft rot ; Disease resistance ; Chinese cabbage ; Protoplast fusion ; Brassica rapa ; B. oleracea ; B. napus ; Erwinia carotovora subsp. carotovora
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Erwinia soft rot is a destructive disease of Brassica rapa vegetables. Reliable sources of resistance and control methods are limited, so development of highly resistant breeding lines is desirable. Protoplasts from B. rapa and B. oleracea genotypes selected for resistance to soft rot were fused in order to combine different sources of resistance. Twelve somatic hybrids (synthetic B. napus) were obtained and confirmed by morphology, nuclear DNA content, and RAPD analysis. They were normal looking plants that easily set seeds following self-pollination and backcrossing to B. rapa. Assays of detached leaves or seedlings inoculated in a mist-chamber showed that most somatic hybrids had lower disease severity ratings than the B. rapa fusion partner and a commercial variety of B. napus. Some progeny from selfing or backcrossing of somatic hybrids to B. rapa showed much more resistance than either fusion partner. The offspring populations of the somatic hybrids (F1–S1 and F1–BC1) clearly moved to the resistant direction compared to the parents; the percentage of resistant plants increased from 21% (average of parents) to 36% (F1–S1) and 48% (F1–BC1). These results suggest that it may be possible to obtain highly resistant B. rapa lines by further backcrossing and selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051356
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Analysis of organelle genomes in a somatic hybrid derived from cytoplasmic male-sterile Brassica oleracea and atrazine-resistant B. campestris (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 303-309 
    Details
    ISSN: 1432-2242
    Keywords: Somatic hybridization ; Atrazine resistance ; Mitochondrial recombinants ; Brassica ; Cytoplasmic male sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An atrazine-resistant, male-fertile Brassica napus plant was synthesized by fusion of protoplasts from the diploid species B. oleracea and B. campestris. Leaf protoplasts from B. oleracea var. italica carrying the Ogura male-sterile cytoplasm derived from Raphanus sativus were fused with etiolated hypocotyl protoplasts of atrazine-resistant B. campestris. The selection procedure was based on the inability of B. campestris protoplasts to regenerate in the media used, and the reduction of light-induced growth of B. oleracea tissue by atrazine. A somatic hybrid plant that differed in morphology from both B. oleracea and B. campestris was regenerated on medium containing 50 μM atrazine. Its chromosome number was 36–38, approximately that of B. napus. Furthermore, nuclear ribosomal DNA from this hybrid was a mixture of both parental rDNAs. Southern blot analyses of chloroplast DNA and an assay involving tetrazolium blue indicated that the hybrid contained atrazine-resistant B. campestris chloroplasts. The hybrid's mitochondrial genome was recombinant, containing fragments unique to each parent, as well as novel fragments carrying putative crossover points. Although the plant was female-sterile, it was successfully used to pollinate B. napus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274711
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Camalexin induction in intertribal somatic hybrids between Camelina sativa and rapid-cycling Brassica oleracea (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 164-170 
    Details
    ISSN: 1432-2242
    Keywords: Key words Intertribal somatic hybrids ; Brassica ; Camelina sativa ; Alternaria ; Camalexin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Camelina sativa, a wild relative of Brassica crops, is virtually immune to blackspot disease caused by Alternaria brassicicola. Intertribal somatic hybrids were produced between C. sativa and rapid-cycling Brassica oleracea as a step toward the transfer of resistance to this disease into Brassica vegetable crops. The plants recovered were confirmed as somatic hybrids by flow cytometry and RAPD analysis. All hybrids showed a morphology intermediate between the two parents. Rooted plants grew in soil up to 4–5 weeks, and some produced sterile flowers. Two of three hybrids tested showed a high level of resistance to  A. brassicicola. Resistance was correlated with the induction of high levels of the phytoalexin camalexin 48 h after inoculation, as in the resistant Camelina fusion partner. In contrast, susceptible somatic hybrids produced much lower levels of camalexin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051053
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  • 6
    Electronic Resource
    Electronic Resource
    Synthesis of high erucic acid rapeseed (Brassica napus L.) somatic hybrids with improved agronomic characters (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 1129-1136 
    Details
    ISSN: 1432-2242
    Keywords: Brassica napus ; somatic hybrids ; protoplast fusion ; Erucic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Novel Brassica napus somatic hybrids have been created through protoplast fusion of B. oleracea var. botrytis and B. rapa var. oleifera genotypes selected for high erucic acid (22:1) content in the seed oil. Fifty amphidiploids (aacc) and one putative hexaploid (aacccc) hybrid were recovered in one fusion experiment. Conversely, only one amphidiploid and numerous regenerates with higher DNA contents were produced in a similar fusion using a different B. rapa partner. Hybridity was confirmed by morphology, isozyme expression, flow cytometry, and DNA hybridization. Analysis of organellar DNA revealed a distinct bias toward the inheritance of chloroplasts from the B. rapa (aa) genome. All amphidiploids set self-pollinated seed. A erucic acid content as high as 57.4% was found in the seed oil of one regenerated plant. Fatty acid composition was stable in the R1 generation and was coupled with increased female fertility. Other novel agronomic characters in the hybrids recovered include large seed size, lodging resistance, and non-shattering seed pods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223931
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  • 7
    Electronic Resource
    Electronic Resource
    Atrazine-resistant cytoplasmic male-sterile-nigra broccoli obtained by protoplast fusion between cytoplasmic male-sterile Brassica oleracea and atrazine-resistant Brassica campestris (1991)
    Springer
    Theoretical and applied genetics 83 (1991), S. 201-208 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Atrazine resistance ; Cytoplasmic male sterility ; Protoplast fusion ; CMS-nigra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplast fusion was used to combine the cytoplasmic traits of atrazine resistance and male sterility in Brassica oleracea var. italica (broccoli). Leaf protoplasts from broccoli with the petaloid B. nigra type of cytoplasmic male sterility were fused with hypocotyl protoplasts from an atrazine-resistant biotype of B. campestris var. oleifera cv Candle (oilseed rape). A total of 19 colonies regenerated shoots, all of which were broccolilike in phenotype, i.e., lacked trichomes. Four shoots, all from one colony, were atrazine resistant, surviving and growing in the presence of 25 μM atrazine. A leaf piece assay also confirmed that they were atrazine resistant. Molecular analysis showed that they contain chloroplasts from the atrazine-resistant B. campestris parent and mitochondria from the B. nigra parent. No recombination or rearrangement of the mitochondrial genomes in the fusion products was detected. These four plants and their progeny all showed the petaloid B. nigra type of male sterility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226252
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  • 8
    Electronic Resource
    Electronic Resource
    Somatic hybrids between Brassica oleracea L. and Sinapis alba L. with resistance to Alternaria brassicae (Berk.) Sacc. (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 1078-1085 
    Details
    ISSN: 1432-2242
    Keywords: Key words Black spot ; Intergeneric hybridization ; protoplast fusion ; white mustard
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Somatic Hybrids between Sinapis alba and rapid-cycling Brassica oleracea were generated for transferring of resistance to Alternaria brassicae to B. oleracea. A. brassicae causes the significant disease black spot in cruciferous crops. A total of 27 plants were regenerated from protoplast fusion using 0, 5, 10, 20 and 30 krad γ-irradiation of the resistance donor and iodoacetate treatment of B. oleracea. All plants showed intermediate morphology with partially divided leaves and some trichomes on stems and leaves. Flow cytometry and banding patterns of the enzymes leucine amino peptidase (LAP) and phosphoglucose isomerase (PGI) confirmed the hybrid status of the regenerated plants. Some of the plants obtained from cuttings from the somatic hybrids showed a resistance to A. brassicae that was similar to that found in S. alba. The flowers of the somatic hybrids had reduced anthers with little pollen production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050518
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    Paper (German National Licenses)
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