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  • Alternaria  (1)
  • Alternaria brassicicola  (1)
  • B. oleracea  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Regeneration of plants from protoplasts of Capsella bursa-pastoris and somatic hybridization with rapid cycling Brassica oleracea (1999)
    Springer
    Plant cell reports 18 (1999), S. 412-417 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsCapsella bursa-pastoris ; Rapid-cycling Brassica oleracea ; Protoplasts ; Intertribal somatic hybrids ; Alternaria brassicicola
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fertile rooted plantlets were recovered from leaf mesophyll protoplasts of Capsella bursa-pastoris. Protoplasts cultured over a feeder layer of Brassica napus cells produced 221 colonies, 7 of which regenerated multiple plantlets. The nuclear DNA content of most regenerates varied from 0.89 to 1.0 pg/nucleus, close to the value for seed-grown C. bursa-pastoris (0.94±0.03 pg/nucleus). Two regenerants had a tetraploid DNA content (1.8– 2.0 pg). Plants with a DNA content close to Capsella produced seeds, both in vitro and in soil. Intertribal somatic hybrids were obtained by polyethylene glycol-mediated fusion of untreated C. bursa-pastoris protoplasts with iodoacetate-treated protoplasts of rapid-cycling B. oleracea. Plants were confirmed as somatic hybrids by isozyme and RAPD analysis. The nuclear DNA content of the hybrids ranged from 3.2 to 6.4 pg, higher than the sum of the parental genomes. One of two hybrids tested was resistant to Alternaria brassicicola, like the Capsella fusion partner. Hybrids rooted easily and produced sterile flowers when transplanted to soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050595
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Improved resistance to bacterial soft rot by protoplast fusion between Brassica rapa and B. oleracea (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 810-819 
    Details
    ISSN: 1432-2242
    Keywords: Key words Bacterial soft rot ; Disease resistance ; Chinese cabbage ; Protoplast fusion ; Brassica rapa ; B. oleracea ; B. napus ; Erwinia carotovora subsp. carotovora
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Erwinia soft rot is a destructive disease of Brassica rapa vegetables. Reliable sources of resistance and control methods are limited, so development of highly resistant breeding lines is desirable. Protoplasts from B. rapa and B. oleracea genotypes selected for resistance to soft rot were fused in order to combine different sources of resistance. Twelve somatic hybrids (synthetic B. napus) were obtained and confirmed by morphology, nuclear DNA content, and RAPD analysis. They were normal looking plants that easily set seeds following self-pollination and backcrossing to B. rapa. Assays of detached leaves or seedlings inoculated in a mist-chamber showed that most somatic hybrids had lower disease severity ratings than the B. rapa fusion partner and a commercial variety of B. napus. Some progeny from selfing or backcrossing of somatic hybrids to B. rapa showed much more resistance than either fusion partner. The offspring populations of the somatic hybrids (F1–S1 and F1–BC1) clearly moved to the resistant direction compared to the parents; the percentage of resistant plants increased from 21% (average of parents) to 36% (F1–S1) and 48% (F1–BC1). These results suggest that it may be possible to obtain highly resistant B. rapa lines by further backcrossing and selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051356
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Camalexin induction in intertribal somatic hybrids between Camelina sativa and rapid-cycling Brassica oleracea (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 164-170 
    Details
    ISSN: 1432-2242
    Keywords: Key words Intertribal somatic hybrids ; Brassica ; Camelina sativa ; Alternaria ; Camalexin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Camelina sativa, a wild relative of Brassica crops, is virtually immune to blackspot disease caused by Alternaria brassicicola. Intertribal somatic hybrids were produced between C. sativa and rapid-cycling Brassica oleracea as a step toward the transfer of resistance to this disease into Brassica vegetable crops. The plants recovered were confirmed as somatic hybrids by flow cytometry and RAPD analysis. All hybrids showed a morphology intermediate between the two parents. Rooted plants grew in soil up to 4–5 weeks, and some produced sterile flowers. Two of three hybrids tested showed a high level of resistance to  A. brassicicola. Resistance was correlated with the induction of high levels of the phytoalexin camalexin 48 h after inoculation, as in the resistant Camelina fusion partner. In contrast, susceptible somatic hybrids produced much lower levels of camalexin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051053
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    Paper (German National Licenses)
    Fulltext
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