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  • protoplast fusion  (2)
  • Alternaria  (1)
  • B. napus  (1)
  • Bipolaris  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Studies on the fungal phytotoxin victorin: Structures of three novel amino acids from the acid hydrolyzate (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1370-1374 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Cochliobolus victoriae ; Helminthosporium ; Bipolaris ; Avena
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The host-selective phytotoxin victorin, produced by the fungusCochliobolus victoriae, was found to be at least partially peptidic in nature, and did not contain victoxinine. The exact mass of the M-H ion was measured by FABMS as 795.1877. Derivatives of three major acid hydrolysis products were isolated. The structures of the corresponding amino acids were assigned as 2S,3R-3-hydroxyleucine, 5,5-dichloroleucine, and 3-hydroxylysine. A into victorin by the fungus in vivo.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01949994
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  • 2
    Digitale Medien
    Digitale Medien
    Improved resistance to bacterial soft rot by protoplast fusion between Brassica rapa and B. oleracea (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 810-819 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Bacterial soft rot ; Disease resistance ; Chinese cabbage ; Protoplast fusion ; Brassica rapa ; B. oleracea ; B. napus ; Erwinia carotovora subsp. carotovora
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Erwinia soft rot is a destructive disease of Brassica rapa vegetables. Reliable sources of resistance and control methods are limited, so development of highly resistant breeding lines is desirable. Protoplasts from B. rapa and B. oleracea genotypes selected for resistance to soft rot were fused in order to combine different sources of resistance. Twelve somatic hybrids (synthetic B. napus) were obtained and confirmed by morphology, nuclear DNA content, and RAPD analysis. They were normal looking plants that easily set seeds following self-pollination and backcrossing to B. rapa. Assays of detached leaves or seedlings inoculated in a mist-chamber showed that most somatic hybrids had lower disease severity ratings than the B. rapa fusion partner and a commercial variety of B. napus. Some progeny from selfing or backcrossing of somatic hybrids to B. rapa showed much more resistance than either fusion partner. The offspring populations of the somatic hybrids (F1–S1 and F1–BC1) clearly moved to the resistant direction compared to the parents; the percentage of resistant plants increased from 21% (average of parents) to 36% (F1–S1) and 48% (F1–BC1). These results suggest that it may be possible to obtain highly resistant B. rapa lines by further backcrossing and selection.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051356
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  • 3
    Digitale Medien
    Digitale Medien
    Camalexin induction in intertribal somatic hybrids between Camelina sativa and rapid-cycling Brassica oleracea (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 164-170 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Intertribal somatic hybrids ; Brassica ; Camelina sativa ; Alternaria ; Camalexin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract   Camelina sativa, a wild relative of Brassica crops, is virtually immune to blackspot disease caused by Alternaria brassicicola. Intertribal somatic hybrids were produced between C. sativa and rapid-cycling Brassica oleracea as a step toward the transfer of resistance to this disease into Brassica vegetable crops. The plants recovered were confirmed as somatic hybrids by flow cytometry and RAPD analysis. All hybrids showed a morphology intermediate between the two parents. Rooted plants grew in soil up to 4–5 weeks, and some produced sterile flowers. Two of three hybrids tested showed a high level of resistance to  A. brassicicola. Resistance was correlated with the induction of high levels of the phytoalexin camalexin 48 h after inoculation, as in the resistant Camelina fusion partner. In contrast, susceptible somatic hybrids produced much lower levels of camalexin.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051053
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  • 4
    Digitale Medien
    Digitale Medien
    Synthesis of high erucic acid rapeseed (Brassica napus L.) somatic hybrids with improved agronomic characters (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 1129-1136 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Brassica napus ; somatic hybrids ; protoplast fusion ; Erucic acid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Novel Brassica napus somatic hybrids have been created through protoplast fusion of B. oleracea var. botrytis and B. rapa var. oleifera genotypes selected for high erucic acid (22:1) content in the seed oil. Fifty amphidiploids (aacc) and one putative hexaploid (aacccc) hybrid were recovered in one fusion experiment. Conversely, only one amphidiploid and numerous regenerates with higher DNA contents were produced in a similar fusion using a different B. rapa partner. Hybridity was confirmed by morphology, isozyme expression, flow cytometry, and DNA hybridization. Analysis of organellar DNA revealed a distinct bias toward the inheritance of chloroplasts from the B. rapa (aa) genome. All amphidiploids set self-pollinated seed. A erucic acid content as high as 57.4% was found in the seed oil of one regenerated plant. Fatty acid composition was stable in the R1 generation and was coupled with increased female fertility. Other novel agronomic characters in the hybrids recovered include large seed size, lodging resistance, and non-shattering seed pods.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223931
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  • 5
    Digitale Medien
    Digitale Medien
    Somatic hybrids between Brassica oleracea L. and Sinapis alba L. with resistance to Alternaria brassicae (Berk.) Sacc. (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 1078-1085 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Black spot ; Intergeneric hybridization ; protoplast fusion ; white mustard
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Somatic Hybrids between Sinapis alba and rapid-cycling Brassica oleracea were generated for transferring of resistance to Alternaria brassicae to B. oleracea. A. brassicae causes the significant disease black spot in cruciferous crops. A total of 27 plants were regenerated from protoplast fusion using 0, 5, 10, 20 and 30 krad γ-irradiation of the resistance donor and iodoacetate treatment of B. oleracea. All plants showed intermediate morphology with partially divided leaves and some trichomes on stems and leaves. Flow cytometry and banding patterns of the enzymes leucine amino peptidase (LAP) and phosphoglucose isomerase (PGI) confirmed the hybrid status of the regenerated plants. Some of the plants obtained from cuttings from the somatic hybrids showed a resistance to A. brassicae that was similar to that found in S. alba. The flowers of the somatic hybrids had reduced anthers with little pollen production.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050518
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