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  • 1
    Electronic Resource
    Electronic Resource
    Ionic dependence of amino-acid transport in the exocrine pancreatic epithelium: Calcium dependence of insulin action (1987)
    Springer
    The journal of membrane biology 96 (1987), S. 153-163 
    Details
    ISSN: 1432-1424
    Keywords: amino-acid transport ; insulin ; diabetes ; calcium dependency ; ouabain ; 2-methylaminoisobutyric acid ; exocrine pancreas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Rapid unidirectional transport (15 sec) ofl-serine and 2-methylaminoisobutyric acid (MeAIB) was studied in the isolated perfused rat pancreas using a dual-tracer dilution technique. Time-course experiments in the presence of normal cation gradients revealed a time-dependent transstimulation ofl-serine influx and transinhibition of MeAIB influx. Transport of the model nonmetabolized System A analog MeAIB was Na+ dependent and significantly inhibited during perfusion with 1mm ouabain. Although transport ofl-serine was largely Na+ independent, ouabain caused a time-dependent inhibition of transport. Influx of both amino acids appeared to be inhibited by the ionophore monensin but unaffected by a lowered extracellular potassium concentration. Removal of extracellular calcium had no effect on influx of the natural substratel-serine, whereas stimulation of transport by exogenous insulin (100 μU/ml) was entirely dependent upon extracellular calcium and unaffected by ouabain. Paradoxically, exogenous insulin had no effect on the time-course of MeAIB influx. The characteristics ofl-serine influx described in earlier studies together with our present findings suggest that insulin may modulate the activity of System asc in the exocrine pancreatic epithelium by a calcium-dependent mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01869241
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  • 2
    Electronic Resource
    Electronic Resource
    Paradoxical effects of endogenous and exogenous insulin on amino acid transport activity in the isolated rat pancreas: somatostatin-14 inhibits insulin action (1989)
    Springer
    Diabetologia 32 (1989), S. 177-184 
    Details
    ISSN: 1432-0428
    Keywords: Amino acid transport ; insulin ; somatostatin ; cholecystokinin octapeptide ; pancreatic secretion ; glucose challenge ; exocrine pancreas (rat)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Regulatory effects of insulin, somatostatin and cholecystokinin on amino acid transport in the isolated perfused rat pancreas have been studied using a rapid dual isotope dilution technique. Uni-directional L-serine transport (15 s) was quantified relative to an extracellular tracer D-mannitol over a wide range of substrate concentrations. In pancreata perfused with 2.5 mmol/l D-glucose, a weighted nonlinear regression analysis of overall transport indicated an apparent Km=14.4±1.6 mmol/l and Vmax=25.9±1.4 μmol ·min−1·g−1 (n=6). Although L-serine transport was stimulated during perfusion with 100 μU/ml bovine insulin, endogenous insulin (7–25 ng·min−1·g−1) released during continuous perfusion with either 8.8 mmol/l or 16.8 mmol/l D-glucose had no such effect. Exogenous somatostatin-14 (250 pg/ml) or cholecystokinin octapeptide (CCK-8, 3 × 10−11mol/l) appeared to increase only the Km for transport. Only CCK-8 evoked a notable protein output (2.9±0.3 mg·30min−1·g−1) and juice flow (68±10μl·30min−1·g −1, n=3) from the exocrine pancreas. When pancreata were perfused with bovine insulin (100 μU/ml) and somatostatin-14 (250 pg/ml), the stimulatory action of exogenous insulin on L-serine transport was abolished. If endogenous insulin and somatostatin, released concurrently in response to 16.8 mmol/l D-glucose, were conveyed to the exocrine epithelium via an islet-acinar portalaxis, it is conceivable that somatostatin modulates the stimulatory action of insulin on basolateral amino acid transport in the exocrine pancreas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265091
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  • 3
    Electronic Resource
    Electronic Resource
    Autoradiographic localization of transported neutral amino acids in epithelia of cat submandibular and sublingual salivary glands (1986)
    Springer
    Cell & tissue research 246 (1986), S. 353-358 
    Details
    ISSN: 1432-0878
    Keywords: Amino acid uptake ; Autoradiography ; Salivary gland ; Serine ; Phenylalanine ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Light-microscopic autoradiography was used to localize the cellular sites for neutral amino acid uptake in submandibular and sublingual salivary gland epithelia. The vasculature of isolated glands was perfused for 3–5 min with either L-(3-3H)serine or L-(4-3H)phenylalanine and then fixed by perfusion with buffered glutaraldehyde. In the submandibular gland the small neutral amino acid L-serine and the aromatic amino acid L-phenylalanine were localized to central acinar cells, demilunar cells and ductal cells. In the sublingual gland silver grains associated with each of these tritiated amino acids were localized to central acinar and ductal cells. Perfusion of both submandibular and sublingual glands with unlabelled L-serine (25 mM) or L-phenylalanine (30 mM) resulted in a significant decrease in the silver grain density associated with each labelled amino acid. The absence of silver grains in the lumina of acinar and ductal cells and the presence of tight junctions near the apical surface of the epithelium strongly suggest that the initial uptake of these amino acids was mediated by basolateral plasma membrane carriers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215897
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  • 4
    Electronic Resource
    Electronic Resource
    Untersuchungen an Alkylmetallalkoxiden der Elemente Aluminium, Gallium und Indium. I. Die Schwingungsspektren von Dimethylaluminiummethoxid und Methylmethoxialuminiumchlorid (1973)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 398 (1973), S. 231-240 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Studies on Alkyl Metal Alkoxides of Aluminium, Gallium, and Indium. I.The Vibrational Spectra of Dimethylaluminiummethoxide and Methylmethoxyaluminiumchloride.The vibrational spectra (IR and RAMAN) of dimethylaluminium methoxide and deuteromethoxide have been measured and assigned. These trimeric alkoxides consist of puckered six-membered AL3O3 ring skeletons probably of C3 or C2 symmetry. The structure of methylmethoxyaluminiumchloride could not be determined definitely from the spectroscopic data.
    Notes: Die Schwingungsspektren (IR u. RAMAN) yon Dimethylaluminiummethoxid und deuteromethoxid Wurden aufgenommen und zugeordnet. Diese trimeren Alkoxide besitzen sechsgliedrige gewellte Al2O3-Ringgerüste wahrscheinlich der Symmetric C2 oder C2. Für Methylmethoxialuminiumchlorid ist die Strukturermittlung schwingungsspektroskopisch nicht eindeutig durchzuführen.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19733980303
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  • 5
    Electronic Resource
    Electronic Resource
    Untersuchungen an Alkylmetallalkoxiden der Elemente Aluminium, Gallium und Indium. III. Eigenschaften und Schwingungsspektren von Dialkylgallium- und Indiumalkoxiden (1974)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 410 (1974), S. 195-205 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Studies on Alkyl Metal Alkoxides of Aluminium, Gallium, and Indium. III. Properties and Vibrational Spectra of Dialkylgallium- and Indium AlkoxidesThe preparation and the properties of dialkyl metal alkoxides R2MOR′ (with R = CH3, CD3, C2H5; R′ = CH3, CD3 and M = Ga, In) are described. The vibrational data (IR and Raman) of the trimeric methyl derivatives point to puckered six-membered M3O3-ring systems.
    Notes: Die Darstellung und die Eigenschaften der Dialkylmetallalkoxide R2MOR′ (mit R = CH3, CD3, C2H5; R′ = CH3, CD3 und M = Ga, In) werden beschrieben. Die schwingungsspektroskopischen Daten (IR und Raman) der trimeren Methylderivate sind mit gewellten M3O3-Sechsringsystemen zu vereinbaren.
    Additional Material: 7 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19744100212
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