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  • Aminophospholipid translocase  (2)
  • Scleroderma  (2)
  • 1
    ISSN: 1432-069X
    Keywords: In vitro study ; Procollagen peptide ; Collagen synthesis ; Scleroderma ; Fibroblastenkultur ; Procollagenpeptide ; Collagensynthese ; Scleroderma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Skerodermie-Fibroblasten einer Patientin im sklerotischen Stadium zeigten unter in vitro Bedingungen eine erhöhte DNA-Syntheserate und eine gesteigerte Kollagensynthese. Durch Zugabe von Prokollagenpeptiden zum Inkubationsmedium konnte die Kollagensythese auf nahezu normales Niveau gebracht werden. Dieses Ergebnis läßt den Schlußzu, daß die Kontrolle der Kollagensynthese über einen »feed-back«-Mechanismus intakt ist. Es ist deshalb möglich, daß der Gehalt an Prokollagenpeptiden selbst in den Sklerodermie-Fibroblasten erniedrigt ist.
    Notes: Summary Fibroblasts derived from a skin biopsy of a patient with scleroderma in the sclerotic stage were shown to have a higher rate of DNA synthesis, and to synthesize more collagen than fibroblasts from a healthy control. The addition of procollagen peptides to the culture medium of scleroderma fibroblasts almost normalized the collagen synthesis. This observation indicates that the mechanism for the regulation of collagen synthesis by feed back inhibition of prollagen peptides is functioning in this disease. It is suggested that the level of biologically active procollagen peptides is lowered.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-069X
    Keywords: Collagen synthesis ; Fibroblasts ; Scleroderma ; Kollagensynthese ; Fibroblast ; Sklerodermie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Fibroblasten wurden aus Hautbiopsien von Patienten mit unterschiedlich aktiven Formen der progressiven systemischen Sklerodermie gewonnen und in vitro kultiviert. Nach 24stündiger Markierungszeit mit 3H-Prolin wurde die Gesamtsynthese des Kollagens gemessen sowie nach Veränderungen im Kollagentypenverhältnis gesucht. Während das Verhältnis von Typ-I-zu Typ-III-Kollagen in alle Stadien der Patienten dem gesunder Kontrollen vergleichbar war, zeigte sich in dem aktiven Stadium im allgemeinen eine deutlich erhöhte Kollagensynthese. Fibroblastenkulturen, die durch eine normale Kollagensynthese charakterisiert waren, ließen sich weder durch Zugabe von Kontroll-noch von Sklerodermie-Patientenserum zu einer höheren Kollagensynthese stimulieren.
    Notes: Summary Fibroblasts were cultured from skin biopsies of patients with systemic sclerosis in different stages of the disease. In vitro synthesis of collagen was checked after a pulse with tritiated proline. The ratio between type I and type III collagen was normal in all patients. Six of seven cultures derived from patients in the active state showed an increased synthesis of collagen relative to other proteins. Addition of serum (normal and diseased) to the culture medium did not stimulate synthesis of collagen in any culture with normal collagen synthesis.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1017
    Keywords: Phospholipid asymmetry ; Aminophospholipid translocase ; Transbilayer diffusion ; Lipid flip-flop (erythrocyte membrane)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract A model is presented to simulate transverse lipid movement in the human erythrocyte membrane. The model is based on a system of differential equations describing the time-dependence of phospholipid redistribution and the steady state distribution between the inner and outer membrane monolayer. It takes into account several mechanisms of translocation: (i) ATP-dependent transport via the aminophospholipid translocase; (ii) protein-mediated facilitated and (iii) carrier independent transbilayer diffusion. A reasonable modelling of the known lipid asymmetry could only be achieved by introducing mechanism (iii). We have called this pathway the compensatory flux, which is proportional to the gradient of phospholipids between both membrane leaflets. Using realistic model parameters, the model allows the calculation of the transbilayer motion and distribution of endogenous phospholipids of the human erythrocyte membrane for several biologically relevant conditions. Moreover, the model can also be applied to experiments usually performed to assess phospholipid redistribution in biological membranes. Thus, it is possible to simulate transbilayer motion of exogenously added phospholipid analogues in erythrocyte membranes. Those experiments have been carried out here in parallel using spin labeled lipid analogues. The general application of this model to other membrane systems is outlined.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2013
    Keywords: Sperm cells ; Plasma membrane ; Lipid asymmetry ; Aminophospholipid translocase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The transbilayer movement of aminophospholipids in the plasma membrane of ram sperm cells was investigated using spin-labeled lipid analogues. After incorporation, spin-labeled phospliatidylserine (SL-PS) and phosphatidyl-ethanolamine (SL-PE) rapidly disappeared from the exoplasmic monolayer. Even at lower temperatures (10°C) the inward movement of SL-PE is fast. The initial velocities of the internalization of SL-PS and SL-PE were compared with those of ram and human erythrocytes
    Type of Medium: Electronic Resource
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