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  • 1
    Electronic Resource
    Electronic Resource
    Diagnosis of the type of amyloid in paraffin wax embedded tissue sections using antisera against human and animal amyloid proteins (1986)
    Springer
    Virchows Archiv 408 (1986), S. 649-664 
    Details
    ISSN: 1432-2307
    Keywords: Amyloid ; Potassium permanganate ; Immunoperoxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Different histochemical techniques were compared on paraffin wax embedded tissue sections for routine classification of amyloid; the following methods were used: potassium permanganate, the indirect immunoperoxidase method using polyclonal anti-human amyloid antisera (anti-AA, anti-Aλ, anti-Aκ and anti-AF) and the peroxidase-antiperoxidase (PAP) method using antisera against human, bovine, hamster and canine AA amyloid. Anti-human AA antiserum appeared to be a useful tool in this respect. Polyclonal anti-AL antisera may be helpful in diagnosing AL amyloid, but were of less value than anti-AA serum. Strong cross reactivity between anti-bovine AA antiserum and human AA amyloid deposits was found. This indicates that animal amyloid AA antisera can also be used for the diagnosis of AA amyloid in human tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00705343
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  • 2
    Electronic Resource
    Electronic Resource
    Über unterschiedliche Blutfülle bei Parabiosepartnern (1969)
    Springer
    Clinical and experimental medicine 149 (1969), S. 233-250 
    Details
    ISSN: 1591-9528
    Keywords: Parabiosis ; Parabiotic intoxication ; Parabiose ; Parabioseintoxikation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wurden Parabiosepaare eines reinen, aber nicht homozygoten Albino-Mäuse-Stammes mit homozygoten C57 BL/6-Paaren hinsichtlich ihrer Lebensdauer, der bei beiden Stämmen gefundenen unterschiedlichen Blutfülle, der Morphologie der Parabiosebrücke, der Organhistologie unter Einschluß der Amyloidose und der Milzgewichte miteinander verglichen. Alle Tiere waren nach einer Methode nachBunster u.Meyer vereinigt. Die Gemeinsamkeiten und Unterschiede der unterschiedlichen Blutfülle bei beiden Stämmen werden an Hand eigener Befunde und der Literatur herausgestellt. Für die agonale bzw. postmortale unterschiedliche Blutfülle homozygoter Paare wird als Ursache eine extreme Blutvolumenverschiebung in den überlebenden Partner nachgewiesen. In dem Gefäßsystem des toten Partners findet mehr als das Doppelte des ursprünglichen Volumens Platz.
    Notes: Summary Parabiotic pairs of pure but not imbred albino mice were compaired with highly imbred C57 BL/6 pairs with respect to lifespans, differential blood contents, morphology of parabiotic bridge, histology of organs comprising amyloidosis, and weight of spleens. All animals were combined in accordance with a method ofBunster andMeyer. The common and different characteristics of the differential blood contents of the pairs of the two strains were emphasized. The difference in the blood volumes of imbred pairs in the agony respectively after death of one of the animals was found to be the result of an extreme shift of blood from surviving partner to the dead one. Finaly the blood vessels of the dead partner contained more than the double amount of blood than before.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02044479
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  • 3
    Electronic Resource
    Electronic Resource
    Quantitative Bestimmung des Blutaustausches zwischen homozygoten Parabiosemäusen (1969)
    Springer
    Clinical and experimental medicine 149 (1969), S. 152-165 
    Details
    ISSN: 1591-9528
    Keywords: Parabiosis ; Blood Exchange (in Parabiosis) ; Blood Volume (in Parabiosis) ; Amyloid (Blood Volume in Amyloidosis) ; Parabiose ; Blutaustausch (bei Parabiose) ; Blutvolumen (bei Parabiose) ; Amyloid (Blutvolumen bei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Mit Hilfe51Cr-markierter Erythrocyten wurden bei 15 Parabiosepaaren etwa 6 Wochen nach der Parabioseoperation die absolute Größe des Blutflusses durch die Parabiosebrücke, das gemeinsame Blutvolumen und an 11 Paaren zusätzlich die Einzelblutvolumina gemessen. Die theoretischen Grundlagen und methodischen Einzelheiten dazu werden erläutert. Der Blutfluß von Maus zu Maus beträgt durchschnittlich 17,2 mg/min (s=±8,5), seine Ratenkonstante im Mittel 0,03/min (s=±0,013) und seine Halbwertszeit 22,8 min. Das Blutvolumen des meist amyloidkranken Tieres ist durchschnittlich 56,2% (s=±19) größer als das seines nicht erkrankten Partners (Signifikanz p〈0,02). Das gemeinsame Blutvolumen der Paare, bezogen auf das gemeinsame Körpergewicht, schwankt nur wenig. Es beträgt 5,52% (s=±0,33) bei mit Casein und Casein + Freunds Adjuvans behandelten Paaren und 8,28% (s±0,36) bei Paaren mit artifizieller Osteomyelitis.
    Notes: Summary Using51Cr-labeled erythrocytes the blood flow along the parabiotic junction and the joint blood volume were measured in 15 parabiotic pairs about 6 weeks after operation. In addition to that in 11 pairs the blood volumes of each parabiont were determined. Theoretical basis and methodical details are described. The average blood flow from mouse to mouse is 17.2 mg/min (s=±8.5), with an average rate of 0.03/min (s=±0.013) and a half-time of 22.8 min. One of the partners obtained a usually successfull amyloidprovoking treatment. The blood volumes of those animals were on the average 56,2% larger than those of their untreated — mostly amyloid-free — partners (p〈0.02). The joint blood volume of the pairs in dependence of their joint weights showed a fairly constant rate. For caseinate and caseinate +Freund's adjuvant treated pairs 5.52% (s=±0.33), for pairs with artificial osteomyelitis 8.28% (s=±0.36) was found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02044807
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  • 4
    Electronic Resource
    Electronic Resource
    Die postoperative Entwicklung des Blutflusses durch die Parabiosebrücke und ihr morphisches Äquivalent (1969)
    Springer
    Clinical and experimental medicine 149 (1969), S. 166-181 
    Details
    ISSN: 1591-9528
    Keywords: Parabiosis ; Blood Exchange (in Parabiosis) ; Blood Volume (in Parabiosis) ; 51Cr-Erythrocytes ; Capillar Growth (in Parabiosis) ; Parabiose ; Blutaustausch (bei Parabiose) ; Blutvolumen (bei Parabiose) ; 51Cr-Erythrocyten ; Capillarsprossung (bei Parabiose)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Mit51Cr-markierten Erythrocyten wird die Entwicklung des Blutflusses zwischen Parabiosetieren an insgesamt 9 Paaren quantitativ verfolgt. Er beginnt um die 50. Stunde. Nach einer starken Zunahme während des Stadiums der Capillarsprossung (70.–80. Stunde) geht der Zuwachs des Blutflusses etwa in eine Sättigungskurve über. Die Analyse von 17 Paaren ergab eine signifikante Korrelation zwischen dem Blutfluß und der Vascularisation der Parabiosebrücke. Auf die Bedeutung dieser Beobachtung zum quantitativen Studium der Wundheilung und auf die genaue Blutvolumenregulation in der Parabiose trotz eines hochsignifikanten Volumenzuwachses während der Amyloidprovokation wird besonders hingewiesen.
    Notes: Summary Using51Cr-labeled erythrocytes in 9 pairs the increase of blood flow between parabiotic animals was measured quantitatively. The blood flow starts about 50 hours after pairing the animals. During the period of capillare growth (70.–80. hours) strong increase of blood flow was observed. Further the augmentation decreased with time of junction. The analysis of 17 pairs shows a significant correlation between blood flow and vascularisation of the parabiotic bridge. In the course of amyloid-provocation blood volume increased highly significant. The importance of these results was pointed out with reference to the problem of woundhealing and exact regulation of blood volume during parabiosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02044808
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