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  • 1
    ISSN: 1432-069X
    Keywords: Key words CTCL ; Sézary syndrome ; CD7 ; Cytokines ; Clonality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The expansion of CD4+CD7– T cells in the peripheral blood of Sézary syndrome (SS) is well known. It remains unclear whether this population contains the dominant T cell clone. Peripheral blood mononuclear cells (PBMC) of five SS patients were sorted by fluorescence-activated cell sorting into CD4+CD7– and CD4+CD7+ populations. These populations were analysed separately for clonality of the T cell receptor γ chain (TCR-γ) by PCR-DGGE. The cytokine profile of both populations was investigated by RT-PCR ELISA for IFN-γ, IL-2, IL-4, IL-5, IL-10, IL-13 and IL-15. In three other patients with known Vβ-usage, the dominant T cell clones were phenotypically characterized by double staining. PCR-DGGE of TCR-γ demonstrated that all patients had a clonal population in their blood and that this population was present in CD4+CD7– and CD4+CD7+ populations. Concerning mRNA cytokine transcription, the two populations did not show any consistent differences. In three patients with identified clones (Vβ 3.1, 5.3 and 6.7), double staining revealed positivity for CD2, CD3, CD4, CD5, CD45RO and CD7 in a significant proportion (at least 35%). We conclude that the CD4+CD7– population does not represent the dominant T cell clone in patients with SS. An increase in this population of PBMC in SS might account for deviations in the T cell functions of the patients.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-072X
    Keywords: Water soluble vitamins (secretion, cell content) ; Chlamydomonas ; Chlorella ; Scenedesmus ; Anabaena
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three green algae, Chlamydomonas reinhardii, Chlorella vulgaris and Scenedesmus obliquus, and one blue-green alga, Anabaena cyclindrica, were grown in chemically defined media. All the algac examined contained folates, β-carotene and vitamins C and E; several of the B-vitamins and vitamin A were found in varying amounts in some but not in all the algae examined. All the green algae secreted significant amounts of folate and biotin and all but Scenedesmus secreted pantothenate into their growth medium; Anabaena secreted folate and pantothenate.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of classification 6 (1989), S. 177-193 
    ISSN: 1432-1343
    Keywords: Partition ; Split ; Dendrogram ; Dual graph ; Complexity ; Polynomial algorithm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Description / Table of Contents: Résumé SoientN objets à classifier et une matrice de dissimilarit és entre paires de ces objets. L'écart d'une classe est la plus petite dissimilarité entre un objet de cette classe et un objet en dehors d'elle. L'algorithme du lien simple fournit des partitions enM classes dont le plus petit écart est maximum. On étudie l'écart moyen des classes, ou, ce qui est équivalent, la somme des écarts. On propose un algorithme en Θ(N 2) pour déterminer des partitions enM classes dont la somme des écarts est maximum pourM allant deN − 1 à 2, basé sur le graphe dual du dendrogramme de la méthode du lien simple.
    Notes: Abstract ConsiderN entities to be classified, and a matrix of dissimilarities between pairs of them. The split of a cluster is the smallest dissimilarity between an entity of this cluster and an entity outside it. The single-linkage algorithm provides partitions intoM clusters for which the smallest split is maximum. We study here the average split of the clusters or, equivalently, the sum of splits. A Θ(N 2) algorithm is provided to determine maximum sum-of-splits partitions intoM clusters for allM betweenN − 1 and 2, using the dual graph of the single-linkage dendrogram.
    Type of Medium: Electronic Resource
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