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  • Chemistry  (34,467)
  • Life and Medical Sciences  (5,983)
  • Analytical Chemistry and Spectroscopy  (4,535)
  • Atomic, Molecular and Optical Physics  (801)
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  • 1
    Electronic Resource
    Electronic Resource
    Dielectric investigation of isotropic and anisotropic solutions of hydroxypropyl cellulose in dioxan (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Polymer International 34 (1994), S. 363-367 
    Details
    ISSN: 0959-8103
    Keywords: hydroxypropyl cellulose ; dioxan ; dielectric behaviour ; refractive index ; isotropy ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The dielectric behaviour of hydroxypropyl cellulose in dioxan has been studied at 10-50°C over a range of concentration of 10-55 wt% to include the isotropic and anisotropic phases. The study showed that the loss maximum ε″max magnitude of polarization ε0 - ε∞ relaxation time 1/2πfm degree of broadening of the absorption curves 1-h or α, and the mean-square dipole moment 〈gμ2〉, steadily increase with concentration up to 42 wt%, above which a rapid decrease takes place. This indicates that the isotropic solution transforms to an anisotropic solution with a smaller mean dipole moment. The critical concentration is realized to be temperature invariant. This was evidenced by measuring the refractive index of solutions covering the same concentration and temperature ranges.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pi.1994.210340402
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  • 2
    Electronic Resource
    Electronic Resource
    Dielectric investigation of isotropic and anisotropic solutions of cellulose acetate in dioxane (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Polymer International 37 (1995), S. 27-32 
    Details
    ISSN: 0959-8103
    Keywords: cellulose acetate ; dioxane solutions ; dielectric behaviour ; anisotropy ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The dielectric and optical characteristics of a sample of cellulose acetate (DS = 2.45) in dioxane solutions were studied at 10-50°C of concentration 10-50 wt% to include both isotropic and anisotropic phases. The study showed that the loss maximum, εmax″, magnitude of polarization, (ε0 - ε∞), static dielectric constant, ε0, time of relation, (2πfm)-1, and refractive index, nD, steadily increase with concentration up to the critical concentration (41 wt%) and then decrease. The mean-square dipole moment, 〈gμ2〉, decreases steadily up to the critical concentration then remains nearly constant, indicating that the isotropic solution changes to anisotropic, with smaller mean-square dipole moment. Comparison between the results of cellulose acetate (CA) and those of hydroxypropyl cellulose (HPC) reveals that, at the critical concentration in dioxane, the cholesteric structure of HPC possesses a greater mean-square dipole moment with higher temperature coefficient than does CA. The activation energy of the relaxation process for hydroxypropyl cellulose is higher, indicating a greater intrachain interaction compared with cellulose acetate.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pi.1995.210370103
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  • 3
    Electronic Resource
    Electronic Resource
    CHO DUKX cell lineages preadapted to growth in serum-free suspension culture enable rapid development of cell culture processes for the manufacture of recombinant proteins (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 52 (1996), S. 518-528 
    Details
    ISSN: 0006-3592
    Keywords: CHO cells ; serum-free medium ; adaptation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Using an adaptive strategy, Chinese hamster ovary (CHO) cell lines were developed that are capable of robust growth in serum-free suspension culture. These preadapted derivatives of the commonly used strain of CHO cells (CHO DUKX), termed PA-DUKX, were used for the introduction and stable expression of several heterologous human genes. A significant advantage of recombinant PA-DUKX cells was their ability to readily resume growth in serum-free suspension culture after transfection and amplification of heterologous genes. Expression of recombinant human proteins in PA-DUKX cells was quantitatively similar to that of lineages generated using conventional CHO DUKX cells. In addition, recombinant human proteins expressed by transfected PA-DUKX lineages were shown to be biochemically and structurally similar to those expressed in CHO DUKX cells, PA-DUKX host cell technology provides an opportunity for reducing the time and resources required to develop large-scale, suspension culture-based manufacturing processes employing serum-free medium. © 1996 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Copolymerization and interaction of acrylonitrile and indene (1989)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 27 (1989), S. 185-191 
    Details
    ISSN: 0887-624X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The donor-acceptor interaction of acrylonitrile (AN) with indene (In) has been investigated by means of ultraviolet spectroscopy and dielectric polarization measurements. The latter method yielded a value of 0.5 for the association constant of the charge-transfer-complex (CTC) and a 4.02 D for its dipole moment. The copolymerization of the two monomers was characterized by a tendency towards alternation when conducted in the bulk. This might be due to the participation of a weak CTC in the process of copolymerization. However, this tendency disappeared in polar solvents such as DMF.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1989.080270116
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  • 5
    Electronic Resource
    Electronic Resource
    Positively and negatively charged water cluster ions generated via liquid chromatography/thermospray mass spectrometry (1991)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 5 (1991), S. 188-191 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The formation of singly protonated water clusters in the positive-ion mode and singly deprotonated water clusters as well as singly negatively charged water clusters in the negative-ion mode with a thermospray system running on pure water and in the discharge-on mode is described. The influence of the potential at the repeller electrode opposite to the sampling cone recalls previous mechanistic discussions on the repeller influence. Application of these water cluster ions to tuning and calibration in the thermospray mode is proposed.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290050409
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  • 6
    Electronic Resource
    Electronic Resource
    Sequencing electroblotted proteins by tandem mass spectrometry (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 1051-1055 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Electroblotting proteins separated by gel electrophoresis provides a suitable support for further manipulations and analysis of small amounts of relatively pure samples. On-membrane digestion, peptide mapping by mass spectrometry, and database searching offer sensitive and fast tools to identify the analyte. By providing sequence information, tandem mass spectrometry can go a step further, confirming the database identification, solving problems connected with post-translational modifications and sequence variations, or supplying the stretches of internal sequence necessary to synthesize an oligonucleotide probe for gene isolation. The viability of this approach was successfully evaluated using different tandem mass spectrometric techniques: metastable decomposition in a matrix-assisted laser desorption/ionization (MALDI) time-of-flight instrument with a curved-field reflectron; low energy collision-induced dissociation in a MALDI quadrupole ion trap mass spectrometer; and high energy collision-induced dissociation in a high-performance four-sector mass spectrometer with massive cluster-impact ionization.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290091116
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  • 7
    Electronic Resource
    Electronic Resource
    The effects of α-amanitin and cycloheximide on nuclear progression, protein synthesis, and phosphorylation during bovine oocyte maturation in vitro (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 28 (1991), S. 249-254 
    Details
    ISSN: 1040-452X
    Keywords: Cow ; In vitro maturation ; Inhibitors ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Bovine cumulus oocyte complexes were cultured for various periods and either denuded and orcein stained or radiolabeled with 35S-methionine or 32P-orthophosphate. Specific inhibitors were added to the culture medium to investigate mRNA and protein synthesis requirements for both nuclear and cytoplasmic changes during maturation in vitro. Inhibition of mRNA synthesis by α-amanitin during the first 2 h of culture prevented the phosphorylation of some specific proteins preceding GVBD and decreased the occurrence of GVBD from 97% to 27%. In addition, in oocytes that had undergone GVBD, only part of the changes in protein synthesis after GVBD were observed. Addition of α-amanitin after 3 h of culture had no effect on meiotic maturation. When cumulus oocyte complexes were cultured in the presence of cycloheximide, the phosphorylation of specific proteins was also blocked and only 5% of the oocytes underwent GVBD. Addition of cycloheximide after 4, 6, or 8 h of culture resulted in an increasing percentage of GVBD, but the oocytes became arrested in metaphase I. When cycloheximide was added from 12 h of culture onwards, nuclear progression to metaphase II was increasingly restored.It is concluded that after the onset of culture, both mRNA and protein synthesis are necessary for the phosphorylation of specific proteins and for GVBD. Further-more, transcription during the first hours of culture is needed for the synthesis of new proteins after GVBD.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080280306
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  • 8
    Electronic Resource
    Electronic Resource
    Analysis of protein synthesis in morphologically classified bovine follicular oocytes before and after maturation in vitro (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 26 (1990), S. 222-226 
    Details
    ISSN: 1040-452X
    Keywords: Cow ; Classification ; Cumulus oocyte complexes ; In vitro maturation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Bovine cumulus oocyte complexes (COCs) were isolated from antral ovarian follicles (4-8 mm). Immature COCs were classified into four categories, based on the homogeneity and clearness of the ooplasm and the transparency and compactness of the cumulus investment. In this study, the incorporation of TCA-precipitable 35S-methionine and the protein synthesis patterns of oocytes of these four categories were examined. Before maturation in vitro, similar incorporation rates and identical protein synthesis patterns were observed between oocytes of categories 1-3. Immature oocytes of category 4 showed reduced incorporation rates and exhibited aberrant protein synthesis patterns. After maturation in vitro, the patterns of category 4 oocytes were identical with the patterns of those in categories 1-3. The incorporation of 35S-methionine into in vitro matured oocytes was lower (P 〈 .001) in all categories.Based on these results, it is concluded that the initial classification of oocytes into four categories can be reduced to two categories.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080260305
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  • 9
    Electronic Resource
    Electronic Resource
    Protein synthesis and phosphorylation patterns of bovine oocytes maturing in vivo (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 29 (1991), S. 271-275 
    Details
    ISSN: 1040-452X
    Keywords: Cow ; Maturation ; Oocyte ; Protein phosphorylation ; Superovulation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: To investigate protein synthesis and phosphorylation during bovine oocyte maturation in vivo, oocytes were collected at consecutive times after the preovulatory luteinizing hormone (LH) peak. Therefore, heifers treated for superovulation were ovariectomized between 3 and 20 h after the maximum of the LH peak. Subsequently, cumulus-enclosed oocytes, selected from nonatretic follicles 〉10 mm, were radiolabeled with 35S-methionine or 32P-orthophosphate for 3 h and individually prepared for gel electrophoresis. Changes in the protein synthesis patterns were observed coinciding with germinal vesicle breakdown (GVBD). No changes were detected during the ensuing maturation period or coinciding with the extrusion of the first polar body. In addition, the protein phosphorylation patterns exhibited striking differences around GVBD. In particular, a phosphoprotein band of 19 kDa and the two heavily phosphorylated proteins with apparent molecular weights between 50 and 60 kDa were present in patterns of oocytes in the germinal vesicle stage. The results are discussed in relation to previous data obtained during maturation in vitro.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080290309
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  • 10
    Electronic Resource
    Electronic Resource
    Optothermal properties of fibers. IV. Optical properties of nylon 66 fibers as a function of quenching process (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 60 (1996), S. 1269-1288 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: In this work, optical properties of samples of nylon 66 fibers were quenched in coarse-grained ice after annealing in the temperature range 80-180°C for 1-10 h. Two independent techniques were used to study optical parameters in these fibers. The first technique is the application of the diffraction technique to study the effect of quenching on the swelling factors of these fibers for some different liquids at room temperature of 25 ± 1°C. The second method dealt with the application of multiple-beam Fizeau fringes in transmission to estimate the refractive indices and birefringence of the skin and core of these fibers. The obtained results were utilized to calculate the polarizability per unit volume, isotropic refractive indices, and the Cauchy's dispersion constants. The relationship between n∥,⊥s, n∥,⊥c, n∥,⊥a, Δns, Δnc, Δna, n(iso)s, n(iso)c, and n(iso)a different annealing times and temperatures is given for these fibers. Microinterferograms and curves are also given. © 1996 John Wiley & Sons, Inc.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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