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γ/δ Receptor-expressing T-cell clones from a cutaneous T-cell lymphoma suppress hematopoiesis (1992)

Wilhelm, M. ; Meyer, P. ; Batram, C. ; [et al.]

Springer

Annals of hematology 65 (1992), S. 111-115

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ISSN: 1432-0584

Keywords: Interferon-γ ; Hematopoiesis ; γ/δ ; γ/δ T cells ; Cutaneous T-cell lymphoma

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary Recently we described a cutaneous T-cell lymphoma expressing the γ/δ T-cell receptor [5]. The patient suffering from this lymphoma showed low numbers of myeloid and T cells in peripheral blood, while B and NK cells were relatively increased. In vitro culture of the patient's bone marrow (BM) cells revealed a significant suppression of myeloid/monocyte colony formation (GM-CFU) compared with normal controls. This was not due to infiltration of the BM with lymphoma cells. We speculated that a soluble factor either secreted or induced by the lymphoma cells might be responsible for the marked suppression of hematopoiesis in this patient. From a skin biopsy with infiltrating γ/δ T-lymphoma cells we established T-cell clones bearing the γ/δ T-cell receptor and resembling the phenotype of the lymphoma cells. The supernatant (SN) of these γ/δ T-cell clones reduced the number of colonies in a CFU-GM assay (using normal control BM) in comparison to SN of α/β T-cell clones established from the same biopsy. This suppression was seen mainly on day 7 of culture and was not neutralized by the addition of placenta-CM. The main mediator of this suppression seems to be IFN-γ,since it was detectable in high amounts in the SN of these γ/δ T-cell tumor clones as well as in the serum of the patient. In addition, anti-IFN-γ antibodies can reverse the T-cell SN-mediated suppression of CFU-GM. We conclude that high serum levels of interferon-γ, which is secreted in high amounts from γ/δ T-cells grown from a biopsy of a cutaneous lymphoma, can suppress hematopoiesis.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01695808

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Determination of chemical-shift tensor orientations in methylene groups by separated-local-field NMR (1993)

Schmidt-Rohr, K. ; Wilhelm, M. ; Johansson, A. ; [et al.]

Chichester : Wiley-Blackwell

Organic Magnetic Resonance 31 (1993), S. 352-356

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ISSN: 0749-1581

Keywords: Separated-local-field NMR ; Chemical-shift tensor orientations ; Poly(ethylene oxide) ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: The 13C chemical-shift tensor orientations in the OCH2CH2O moieties of poly(ethylene oxide) (PEO) and poly(ethylene terephthalate) (PET) were determined by means of 1H—13C separated-local-field NMR spectroscopy, and the literature values for the shift tensors in polyethylene (PE) and polyoxymethylene (POM) were improved. An angular resolution of ± 4° was attained on standard equipment with 90° pulse lengths exceeding 4 μs. Relatively high uniformity of the methylene tensor orientations, complying with local molecular symmetry, was found.

Additional Material: 4 Ill.