ISSN:
1573-5044
Schlagwort(e):
callus
;
metal tolerance
;
shoot regeneration
;
Solanaceae
;
tobacco
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract Manganese toxicity to germination, callus induction and shoot regeneration was studied on three cultivars of Nicotiana tabacum: BEL W3, Burley 21, Bright NC 944. All materials were cultured on MS solid medium containing 0.1 (control), 2, 5, 10, 15 and 20 mM Mn, to which 5 µM NAA and 5 µM kinetin were added for callus induction and shoot regeneration. Mn toxicity to callus growth was tested using habituated callus of Nicotiana bigelovii var. bigelovii grown on MS medium without growth regulators. Mn concentrations higher than 2 mM were toxic for germination, and concentrations higher than 5 mM were toxic for callus induction, shoot regeneration and callus growth. Among the cultivars examined, Bright tobacco appeared more tolerant to high Mn concentrations during callus formation and shoot regeneration. However, many regenerated plants capable of growing in vitro in the presence of 2 and 5 mM Mn were obtained.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1023/A:1005917114146
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