ISSN:
0306-042X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
In order to analyse and quantitate the urinary 16-oxysteroids known or thought to be associated with hypertension, we have established for six 16-oxy-C19 reference steroids the following parameters: elution volume on lipophilic gel columns, gas chromatographic retention data expressed as methylene unit values of trimethylsilyl ether and O-methoxime trimethylsilyl ether derivatives on OV-1 and OV-17 packed columuns and on SE-30 capillary column, and mass spectra of these compounds. These reference steroids were: 3α, 16α-dihydroxy-5α-androstan-17 one, 3α,16α-dihydroxy-5β-androstan-17-one, 3β,16α-dihydroxy-5α-androstan-17-one, 3β,16α-dihydroxy-5-androsten-17-one, 3β,16β-dihydroxy-5-androsten-17-one, 3β,17β-dihydroxy-5-androsten-16-one and 3α,15α-dihydroxy-5β-androstan-17-one. The proposed method was shown to be applicable to the specific analysis of 16-oxy-C19-steroids in biological samples since it achieved the selective isolation of these compounds from other steroids and their quantitative elution in a single fraction. The analysis of the urinary steroids of two patients with arterial hypertension demonstrated an elevated rate of 3β,16α-dihydroxy-5-androstan-17-one.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200061104
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