ISSN:
1365-3059
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Genetic diversity among Fusarium moniliforme isolates was analysed using vegetative compatibility group (VCG) and random amplified polymorphic DNA (RAPD) techniques. In the first experiment, RAPD was used to analyse a set of 43 isolates collected from different corn growing areas in Israel and the US. The isolates were assigned to 27 different VCGs. Thirty-two RAPD haplotypes were also detected by analysing 48 polymorphic bands. RAPD could differentiate all the VCGs, except in two cases where two VCGs were assigned a single RAPD haplotype. In six cases, however, molecular variation was detected among isolates belonging to the same VCG. Cluster analysis of the RAPD data showed a very good agreement with the VCG grouping, e.g. isolates of the same VCG were always closely clustered by the molecular data. In a second experiment, 63 isolates of Fusarium moniliforme were collected from six corn lines growing in a single corn field. Extensive genetic variation was observed among the isolates: 42 different VCGs and 37 RAPD haplotypes were identified. Once again, RAPD patterns could differentiate nearly all the VCGs. However, in four cases, two different VCGs were grouped into a single RAPD haplotype, while in another three cases, isolates of the same VCG could be differentiated by distinct molecular haplotypes. The variation data was used to gain insight on the population structure and the patterns of genetic variation among geographical locations and within a single field. Hierarchical gene diversity analysis of the RAPD data indicated that most of the genetic variability (81%) was distributed within corn lines in the same field, suggesting that RAPD haplotype, or VCG frequencies, were not significantly affected by the plant genotypes grown in this experiment. Most of the RAPD band combinations did not display significant gametic phase disequilibrium, suggesting that active recombination might be occurring in the field. Our results indicate that by using a small number of primers, similar resolution was obtained by RAPD and VCG analysis, respectively. RAPD analysis is however, simpler to perform and its sensitivity in genotyping individuals within Fusarium moniliforme can be increased by analysing more primers, enabling a more detailed population genetic analysis of this important pathogen.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1365-3059.1997.d01-91.x
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