ISSN:
1432-0878
Schlagwort(e):
Cerebrospinal fluid-contacting neurons
;
Monoclonal antibodies
;
Opsin
;
VIP
;
Extraocular photoreceptors
;
Ring dove
;
Coturnix coturnix
;
Anas platyrhynchos
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Medizin
Notizen:
Summary Cerebrospinal fluid-contacting (CSF) cells in both the septal and the tuberal areas in the brain of the ring dove are labeled by RET-P1, a monoclonal antibody to opsin that reacts with inner and outer segment membranes of rod photoreceptors in a variety of vertebrates. Immunoblot analysis of proteins from diverse brain regions, however, revealed bands of anti-RET-P1 immunoreactivity that did not correspond to opsin. Binding of RET-P1 to opsin-containing membranes, was not inhibited by membranes rich in muscarinic and β-adrenergic receptor proteins (red blood cells, heart, lung) taken from doves. RET-P1-immunoreactive CSF-contacting cells emit a dendritic process that penetrates the ependyma and ends in a knob-like terminal suspended in the ventricle. These cells also possess other processes that penetrate more or less deeply into the neuropil. Additionally, a band of labeled fibers occurs in the external layer of the median eminence. A double-label technique demonstrated that RET-P1-positive cells coexpress VIP-like immunoreactivity. VIP-positive cells in other brain areas are not RET-P1-positive.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00221754
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