ISSN:
1612-1112
Keywords:
Capillary gas chromatography
;
High performance liquid chromatography
;
GC-MS
;
Impurity profiles
;
17-oxo-5α-androstenes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The impurity profile of 5α-androst-2-ene-17-one was investigated by using two different capillary gas chromatographic (GC) and a reverse phase high performance liquid chromatographic systems (HPLC). The impurities were identified by retention matching with authentic standards, diode-array UV spectroscopy, on-line gas chromatography-mass spectrometry (GC-MS), off-line HPLC-MS and NMR spectroscopy. The following impurities were identified and quantitated: ring A saturated, isomeric (1-ene, 3-ene, 4-ene, 5-ene) analogues, 3α and 3β-hydroxy as well as 3β-phenyl derivatives.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02268173
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