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  • 1
    Electronic Resource
    Electronic Resource
    Malignant chondroblastoma presenting as a recurrent pelvic tumor with DNA aneuploidy and p53 mutation as supportive evidence of malignancy (1999)
    Springer
    Skeletal radiology 28 (1999), S. 644-650 
    Details
    ISSN: 1432-2161
    Keywords: Key words Chondroblastoma ; malignant ; recurrent ; p53 mutation ; Aneuploidy ; Pelvis ; Femur ; Xray ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We report a rare case of malignant chondroblastoma, which presented in a 47-year-old man as a recurrent tumor, 18 years following wide excision of a typical pelvic chondroblastoma. Radiologic studies of the recurrent tumor showed a large, lytic, destructive lesion of the right pelvic bones and femur, with a pathologic fracture of the latter, a large pelvic soft tissue mass, and multiple pulmonary metastases. Biopsy tissue showed typical features of chondroblastoma, but also increased nuclear atypia, hyperchromasia, and pleomorphism, compared to the original tumor, and, most significantly, abnormal mitotic figures. Immunohistochemical studies of the recurrent tumor revealed p53 mutation and extensive proliferative activity, and flow cytometric studies showed DNA aneuploidy, none of which was present in the original tumor. The patient received chemotherapy and radiation, but died of disease eight months after presentation. We also review chondroblastoma in general, to assign this unusual lesion to a tumor subtype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050567
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  • 2
    Electronic Resource
    Electronic Resource
    Filtration-based perfusion of hybridoma cultures in protein-free medium: Reduction of membrane fouling by medium supplementation with DNase I (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 43 (1994), S. 833-846 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In this study, a filtration-based perfusion process was developed for the production of monoclonal antibodies (IgM) by suspended hybridoma cells grown in protein-free medium. It was found that the use of protein-free medium for perfusion culture generated the formation of numerous visible suspended particles consisting of dead cells and cellular debris aggregated into fibrous material. Surprisingly high apparent viabilities were observed in such protein-free cultures. In addition, membrane fouling occurred more rapidly in protein-free medium than in conventional serum-supplemented medium. By the addition of deoxyribonuclease I (DNase I) to the protein-free medium, it was possible to prevent the formation of aggregates and to follow the evolution of the total cell population more accurately. Moreover, DNase I significantly reduced the fouling of filtration membranes, and that, for two different types of separation systems (cross-flow and vortex-flow filtration) and two different types of membranes (polycarbonate and hydrophilized polysultone). From these results, it is clear that the presence of DNA fragments liberated following cellular death is playing an important role in membrane fouling. Longevity of filtration membranes was found to be considerably greater using a vortex-flow filtration module than with a static plate-and-frame cross-flow filtration module. The use of vortex-flow filtration of conjuction with DNase I allowed maintenance of perfusion cultures for more than 1 month without membrane fouling or antibody retention and with a constant permeate IgM concentration of 250 mg/L. Hybridomacells appeared to gradually adapt to increasing rotational speed in the vortex-flow filtration module.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260430902
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  • 3
    Electronic Resource
    Electronic Resource
    13C NMR chemical shift trends and conformational changes in alkali metal cation complexes of a series of naphthalene crown ethers (1988)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 26 (1988), S. 197-203 
    Details
    ISSN: 0749-1581
    Keywords: 13C NMR ; Chemical shifts ; Crown ethers ; Conformations ; Complexes ; Naphthalene ; Sodium ion ; Potassium ion ; Rubidium ion ; Cesium ion ; Binding constants ; Alkali metal cations ; Complexation constants ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 13C NMR studies of alkali metal cation complexation by six related crown ethers are described. The crown ethers (and ions studied) were 2,3-naphtho-20-crown-6 (Na+, K+, Rb+, Cs+), 2,3-naphtho-17-crown-5 (Na+, K+, Rb+, Cs+), 2,3-naphtho-14-crown-4 (Na+, K+, Rb+, Cs+), 1,8-naphtho-21-crown-6 (Na+, K+, Rb+, Cs+), 1,8-naphtho-18-crown-5 (Na+, K+, Rb+, Cs+) and 1,5-naphtho-22-crown-6 (K+, Rb+, Cs+). For each crown ethercation combination, 13C NMR spectra were obtained for a number of different ion concentrations in CD3OD. Data were analysed by a non-linear least-squares treatment to give complexation constants and chemical shifts that would result at the limit of complete 1:1 complexation. Analyses of the limiting chemical shifts for a number of carbons indicate regular, cation-dependent trends (for Cs+ through Na+). Deviations from these trends are interpreted as reflecting differences in conformations of crown ether-cation complexes. Conformational differences between cation complexes that occur near the point of naphthalene attachment cause deviations from the trend in the aromatic carbon chemical shifts, while conformational differences further from the naphthalene cause trend deviations only in the crown ether carbon chemical shifts.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260260305
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