ISSN:
1573-6822
Keywords:
B16 melanoma
;
human mononucleated cells
;
HSPs70 induction
;
murine macrophages
;
ozone
;
toxicity tests
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The aim of this work was to study thein vitro effect of ozone on the 70 kDa family of inducible heat shock proteins (HSPs 70). We also performed tests to investigate possible toxic effects of ozone at the different doses employed. In human haematic mononucleated cells ozone at doses up to 20 μg/ml had no toxic effects and induced biosynthesis of the HSPs70. Biosynthesis of these proteins was greater at 40 μg/ml. In murine macrophages testing with tetrazolium salt (MTT), neutral red, and 2-deoxy-d-[1-3H]glucose uptake and study of the cell morphology showed a remarkable resistance or no toxic effects at a dose of 100 μg/ml also. Melanoma B16 murine cells assayed with the MTT test demonstrated less resistance to the toxic effects of ozone than normal cells. These results provide indications relevant to the problems of ozone therapy.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00769988
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