ISSN:
1432-072X
Keywords:
Rhizobium japonicum
;
Rhizobiophage
;
d-Glucosamine
;
Antiphage serum
;
Transduction
;
Markers
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Phage M-1 produced clear plaques with a halo in the lawn of Rhizobium japonicum D211. A one step growth curve of phage M-1 showed a latent period of 3 h, burst size of 55 and rise period of 2 h. The inactivation of phage M-1 was found to be dependent upon the concentraion of d-glucosomanine. The neutralization kinetics of phage M-1 by antiphage serum gave a K value (velocity constant) of 83.1 min−1. Transduction of str and kan was studied in the presence of antiphage serum and d-glucosamine. Cotransduction of different antibiotic resistance markers suggested that the system can be further explored for high resolution mapping in R. japonicum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00459531
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