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  • Restriction fragment length polymorphism  (2)
  • Aphis craccivora  (1)
  • Bambara groundnut  (1)
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  • 1
    Digitale Medien
    Digitale Medien
    Molecular taxonomic relationships in the genus Vigna based on RFLP analysis (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 97-104 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Vigna ; Numerical taxonomy ; RFLP ; Asiatic grams ; Cowpea ; Bambara groundnut
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The taxonomy of the genus Vigna has been primarily based on morphological attributes. We have used 27 genomic clones from soybean, common bean, mungbean and cowpea to examine restriction fragment length polymorphism (RFLP) among 44 accessions of different species belonging to four subgenera of the genus Vigna. One accession each of common bean (Phaseolus vulgaris) and soybean (Glycine max) was included in the study. Total DNA from the various genotypes was digested with one restriction enzyme (Eco RV). Results of a numerical taxonomic analysis showed a high level of genetic variation within the genus with a remarkably higher amount of variation associated with Vigna sp. from Africa relative to those from Asia. The distinctness of the Asiatic grams in subgenus Ceratotropis, cowpea in section Catiang, bambara groundnut (V. subterranean) and members of the subgenus Plectotropis was elucidated by this study. Members of the subgenus Plectotropis were closer in genome homology to those of subgenus Vigna section Catiang than to those of subgenus Ceratotropis. The relative positions of some genotypes to one another on the dendrogram and minimum spanning tree were discussed in regard to hybridisations aimed generating well-saturated genomic maps and interspecies transfer of desirable genes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223813
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  • 2
    Digitale Medien
    Digitale Medien
    High resolution RFLP map around the root knot nematode resistance gene (Mi) in tomato (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 529-536 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Disease resistance ; Meloidogyne incognita ; Lycopersicon esculentum ; Genetic mapping ; Restriction fragment length polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In the 1940's the root-knot nematode resistance gene (Mi) was introgressed into the cultivated tomato from the wild species, L. peruvianum, and today it provides the only form of genetic resistance against this pathogen. We report here the construction of a high resolution RFLP map around the Mi gene that may aid in the future cloning of this gene via chromosome walking. The map covers the most distal nine map units of chromosome 6 and contains the Mi gene, nine RFLP markers, and one isozyme marker (Aps-1). Based on the analysis of more than 1,000 F2 plants from four crosses, we were able to pinpoint the Mi gene to the interval between two of these markers — GP79 and Aps-1. In crosses containing the Mi gene, this interval is suppressed in recombination and is estimated to be 0.4 cM in length. In contrast, for a cross not containing Mi, the estimated map distance is approximately 5 times greater (ca. 2 cM). Using RFLP markers around Mi as probes, it was possible to classify nematode resistant tomato varieties into three types based on the amount of linked peruvianum DNA still present. Two of these types (representing the majority of the varieties tested) were found to still contain more than 5 cM of peruvianum chromosome — a result that may explain some of the negative effects (e.g. fruit cracking) associated with nematode resistance. The third type (represented by a single variety) is predicted to carry a very small segment of peruvianum DNA (〈2 cM) and may be useful in the identification of additional markers close to Mi and in the orientation of clones during a chromosome walk to clone the gene.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226787
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  • 3
    Digitale Medien
    Digitale Medien
    Comparative genome analysis of mungbean (Vigna radiata L. Wilczek) and cowpea (V. unguiculata L. Walpers) using RFLP mapping data (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 797-810 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Parallel maps ; Molecular markers ; Random amplified polymorphic DNA ; Restriction fragment length polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Genome relationships between mungbean (Vigna tradiata) and cowpea (V. Unguiculata) based on the linkage arrangement of random genomic restriction fragment length polymorphism (RFLP) markers have been investigated. A common set of probes derived from cowpea, common bean (Phaseolus vulgaris), mungbean, and soybean (Glycine max) PstI genomic libraries were used to construct the genetic linkage maps. In both species, a single F2 population from a cross between an improved cultivar and a putative wild progenitor species was used to follow the segregation of the RFLP markers. Approximately 90% of the probes hybridized to both mungbean and cowpea DNA, indicating a high degree of similarity in the nucleotide sequences among these species. A higher level of polymorphism was detected in the mungbean population (75.7%) than in the cowpea population (41.2%). Loci exhibiting duplications, null phenotypes, and distorted segregation ratios were detected in both populations. Random genomic DNA RFLP loci account for about 89% of the currently mapped markers with a few cDNA and RAPD markers added. The current mungbean map is comprised of 171 loci/loci clusters distributed in 14 linkage groups spanning a total of 1570cM. On the other hand, 97 markers covered 684 cM and defined 10 linkage groups in the current cowpea map. The mungbean and cowpea genomes were compared on the basis of the copy number and linkage arrangement of 53 markers mapped in common between the two species. Results indicate that nucleotide sequences are conserved, but variation in copy number were detected and several rearrangements in linkage orders appeared to have occurred since the divergence of the two species. Entire linkage groups were not conserved, but several large linkage blocks were maintained in both genomes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00212605
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  • 4
    Digitale Medien
    Digitale Medien
    RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata L. Walp) (1996)
    Springer
    Euphytica 91 (1996), S. 181-187 
    Details
    ISSN: 1573-5060
    Schlagwort(e): Aphis craccivora ; cowpea ; DNA markers ; insect ; legume ; restriction fragment length polymorphisms ; Vigna unguiculata
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Restriction fragment length polymorphism (RFLP) analysis has several advantages over traditional methods of genetic linkage mapping, one of these being the starting point for map-based cloning. The recent development of an RFLP map of cowpea (Vigna unguiculata L. Walp) has allowed the investigation of associations between genes of interest and RFLP markers. A cross between an aphid (Aphis craccivora Koch) resistant cultivated cowpea, TT84S-2246-4, and an aphid susceptible wild cowpea, NI 963, was screened for both aphid phenotype and RFLP marker segregation. One RFLP marker, bg4D9b, was found to be tightly linked to the aphid resistance gene (Rac 1) and several flanking markers in the same linkage group (linkage group 1) were also identified. The close association of Rac 1 and RFLP bg4D9b presents a real potential for cloning this insect resistance gene.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00021068
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