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  • 1
    ISSN: 1432-203X
    Keywords: Key words Transgenic tobacco ; Arabidopsis thaliana ; Nitrilase ; NIT genes ; Indole-3-acetic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Arabidopsis thaliana genome has four nitrilase (nitrile aminohydrolase, EC 3.5.5.1) genes (NIT1 to NIT4). These nitrilases catalyze hydrolysis of indole-3-acetonitrile (IAN) to indole-3-acetic acid (IAA). Growth of A. thaliana is inhibited by IAN probably due to hydrolysis of IAN to IAA, while the tobacco (Nicotiana tabacum) genome has only NIT4 homologs and is resistant to IAN. In this study, we introduced A. thaliana NIT1 to NIT4 into tobacco. Introduction of NIT1, NIT2 or NIT3 into tobacco conferred growth inhibition by IAN. NIT2 transgenic plants were highly sensitive to IAN, and NIT1 and NIT3 transgenic plants were moderately sensitive. On the other hand, NIT4 transgenic plants were less sensitive to IAN, although some morphological changes in the roots were observed as the wild-type tobacco. These findings suggest that the ability of transgenic tobacco to convert IAN to IAA in vivo is markedly different among transgenes of NIT1 to NIT4.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1435-2451
    Keywords: Tumor of the extra-biliary tract ; Irradiation and PTD ; Degenerative changes in tumor cells ; Extrahepatische Gallenwegstumore ; Radiatio und PTD ; degenerative Veränderungen der Krebszellen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei Tumoren im Leberhilus handelt es sich meist um Adenocarcinome, die bis jetzt im Gegensatz zu den Plattenepithelcarcinomen oder bösartigen Lymphomen als wenig strahlenempfindlich galten. 27 Patienten nach Ikterusentlastung durch PTD mit anschließender Chemotherapie wurden bestrahlt. Durch Strahlentherapie konnte eine Tumorverkleinerung festgestellt werden, gleichzeitig zeigten sich histologisch degenerative Veränderungen der Krebszellen. Die durchschnittliche Überlebenszeit bei 12 erfolgreichen Patienten beträgt 13,9 Monate.
    Notes: Summary Adenocarcinomas in the hilus of the liver are comparatively less susceptible to radiotherapy than epithelial cell tumor or malignant lymphoma. Twenty-seven patients were treated with radiotherapy after decompression of the hepatobiliary system percutaneous drainage and chemotherapy. Tumors showed reduction in size, and at the same time degenerative changes in tumor cells were seen histologically after this method of treatment. Twelve patients survived to an average of 13.9 months after succesful therapy.
    Type of Medium: Electronic Resource
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