ISSN:
1432-072X
Keywords:
Rhizobium trifolii
;
Aromatic metabolism in catechol 1,2-dioxygenase
;
R. trifolii
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Catechol 1,2-dioxygenase has been purified 46-fold from cells of Rhizobium trifolii TA1 grown on benzoate plus glucose. The dioxygenase had a molecular weight of 107,000 and a sub-unit molecular weight of 59,000. The enzyme had a K m of 2 μM for catechol and also cleaved 4-methylcatechol. The dioxygenase contained 2 g atoms of Fe3+ per mole of enzyme which could be removed by treatment with 1,10-phenanthroline, resulting in a complete loss of activity; reactivation of the enzyme occurred specifically with Fe3+.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00408063
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