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Digitale Medien

Utilisation of organic nitrogen and phosphorus sources by mycorrhizal endophytes of Woollsia pungens (Cav.) F. Muell. (Epacridaceae) (1999)

Chen, A. ; Chambers, S. M. ; Cairney, J. W. G.

Springer

Mycorrhiza 8 (1999), S. 181-187

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ISSN: 1432-1890

Schlagwort(e): Key words Ericaceae ; Hymenoscyphus ericae ; Ericoid mycorrhiza ; Plant nutrition ; Ascomycetes

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract The ability of four ericoid mycorrhizal endophytes isolated from roots of Woollsia pungens (Cav.) F. Muell. (Epacridaceae) to utilise organic forms of nitrogen and phosphorus during growth in axenic culture was assessed. All isolates were able to utilise glutamine, arginine and bovine serum albumin (BSA), along with NH4 + or NO3 –, in most cases yielding at least as much biomass as the ericoid mycorrhizal endophyte Hymenoscyphus ericae (Read) Korf & Kernan. All isolated endophytes were able to utilise BSA, arginine and glutamine as sole sources of N and C. With the exception of a single isolate (C40), which showed little growth on glutamine, biomass yields on glutamine as the sole N and C source was significantly greater for all isolates than on either of the other two organic N sources. Two isolates from W. pungens (C40 and A43) utilised DNA and sodium inositol hexaphosphate as sole P sources, in each case yielding significantly more biomass than H. ericae. The results suggest that mycorrhizal endophytes from epacrid plant hosts and those from ericaceous hosts have similar abilities to utilise organic forms of N and P.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s005720050232

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Digitale Medien

Potentiometric method for subatrate analysis using immobilized NAD+-dependent oxidoreductase enzymes (1979)

Chen, A. K. ; Liu, C. C. ; Schiller, J. G.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 21 (1979), S. 1905-1915

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Two coenzyme-dependent oxidoreductases, glucose dehydrogenase and alcohol dehydrogenase, were immobilized in polyacrylamide gel over a platinum grid matrix and used as enzyme electrodes to measure their substrate concentrations in buffered aqueous solutions. The immobilized enzymes were used to oxidize their substrates in the presence of NAD+. Ferricyanide was used as the redox mediator and electroactive specific. The determinations of glucose and ethenol were utilized to demonstrate and evaluate the performance of the system. The described methodology should be readily applicable to the analysis of numerous other substrates of coenzyme-dependent oxidoreductases.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260211103

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Digitale Medien

Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase (1982)

Chen, A. K. ; Starzmann, J. A. ; Liu, C. C.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 24 (1982), S. 971-975

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD+ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10-4M to 10-1M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.

Zusätzliches Material: 1 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260240416

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Digitale Medien

Electrochemical evaluation of lactate dehydrogenase immobilized in polyacrylamide gels (1977)

Chen, A. K. ; Liu, C. C.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 19 (1977), S. 1785-1792

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Lactate dehydrogenase (EC 1.1.1.27) has been immobilized in polyacrylamide gels over a platinum grid matrix. The immobilized enzyme is used to oxidize L-lactate in the presence of nicotinamide adenine dinucleotide (NAD+) and femcyanide. The NADH produced is then chemically oxidized back to NAD+by ferricyanide. The coupled reduction of ferricyanide ions to ferrocyanide ions results in a measurable electrochemical potential. This measurable zero-current potential is found to be Nernstian in nature and directly proportional to the logarithm values of L-Iactate concentration over the range of 2 × 10-5to 5 × 10-2M. The results indicate that immobilized lactate dehydrogenase can be incorporated into a system to detect L-Lactate acid in aqueous solutions.

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260191205

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Digitale Medien

ON THE STRUCTURE OF STEADY FLOW THROUGH DUAL-INTAKE ENGINE PORTS (1996)

MAHMOOD, Z. ; CHEN, A. ; YIANNESKIS, M. ; [weitere]

Chichester : Wiley-Blackwell

International Journal for Numerical Methods in Fluids 23 (1996), S. 1085-1109

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ISSN: 0271-2091

Schlagwort(e): intake port ; dual intake ; CFD ; laser Doppler anemometry ; internal combustion engine ; cylinder ; turbulence ; steady flow ; Engineering ; Engineering General

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Maschinenbau

Notizen: The influence of intake port design on the flow field in a dual-intake valve engine was investigated using computational fluid dynamics, in order to study the effect of inlet port design on the in-cylinder flow. A detailed 3D computational grid incorporating all the features of the Ford Zetec production engine inlet ports, valves and cylinder head was initially created and the flow structure modelled at 5 and 10 mm valve lifts under steady flow conditions. Comparisons of computational results with experimental data obtained by laser Doppler anemometry indicate that the flow characteristics have been predicted well in most regions. Flow generated by different intake port designs was also simulated by introducing air into the cylinder at different directions to the inlet valve axes and the effects of port deactivation, throttling and exhaust gas recirculation were examined. The implications of the results for intake port design are discussed.

Zusätzliches Material: 13 Ill.

Materialart: Digitale Medien

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