Bibliothek

X
feed icon rss

Ihre E-Mail wurde erfolgreich gesendet. Bitte prüfen Sie Ihren Maileingang.

Leider ist ein Fehler beim E-Mail-Versand aufgetreten. Bitte versuchen Sie es erneut.

Vorgang fortführen?

Exportieren
Filter
  • Asparagus  (2)
  • Nicotiana tabacum  (2)
  • Tropical crops  (2)
  • 1
    Digitale Medien
    Digitale Medien
    Survival of cultured cells and somatic embryos of Asparagus officinalis cryopreserved by vitrification (1989)
    Springer
    Plant cell reports 8 (1989), S. 418-421 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Cryopreservation ; Vitrification ; Plant germplasm ; Asparagus ; Asparagus officinalis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Cultured cells and somatic embryos derived from the mesophyll tissue of asparagus (Asparagus officinalis L.) were cryopreserved by vitrification. The vitrification solution (PVS) contains (w/v) 22% glycerol, 15% ethylene glycol, 15% propylene glycol and 7% DMSO in Murashige-Skoog medium enriched with 0.5M sorbitol. After initial cryoprotection with sorbitol supplemented MS medium containing 12% ethylene glycol, cells or embryos were exposed stepwise to 85% PVS at 0°C. They were loaded into 0.5 ml transparent straws, and were then plunged directly into liquid nitrogen. After rapid warming, PVS was removed and diluted stepwise. The highest survivals of vitrified cells and embryos were about 65 and 50%, respectively. Surviving embryos developed into plantlets.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00270083
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 2
    Digitale Medien
    Digitale Medien
    Cryopreservation of in vitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure (1997)
    Springer
    Plant cell reports 16 (1997), S. 594-599 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key words Shoot tips ; Cryopreservation ; Vitrification ; Taro [Colocasia esculenta (L.) Schott.] ; Tropical crops
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In vitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott.) were successfully cryopreserved by vitrification. Excised shoot tips precultured on solidified MS supplemented with 0.3 M sucrose and maintained under a 16 h phtoperiod at 25°C for 16 h were loaded with a mixture of 2 M glycerol plus 0.4 M sucrose for 20 min at 25°C. The shoot tips were then sufficiently dehydrated with a highly concentrated vitrification solution (PVS2) for 20 min at 25°C prior to immersion into liquid nitrogen. Successfully vitrified and warmed shoot tips resumed growth within 7 days and developed shoots directly without intermediate callus formation. The average rate of shoot recovery amounted to around 80%, and the vitrification protocol appeared to be very promising for the cryopreservation of taro germplasm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990050285
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 3
    Digitale Medien
    Digitale Medien
    Cryopreservation of in vitro-cultured multiple bud clusters of asparagus (Asparagus officinalis L. cv Hiroshimagreen (2n=30) by the techniques of vitrification (1992)
    Springer
    Plant cell reports 11 (1992), S. 433-437 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Cryopreservation ; Vitrification ; Asparagus ; In vitro ; cultured bud clusters
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A culture line of asparagus forming green bulbous structures consisting of numerous multiple bud clusters designated “bud clusters” was induced from a meristem culture of asparagus (Asparagus officinalis L.cv. Hiroshimagreen, 2n=30). Small cubic segments (2 mm3) cut from bud clusters were cryopreserved using three different cryogenic protocols. Only vitrification produced very high levels of shoot formation after cooling to −196°C. Segments were treated with a vitrification solution (PVS2) at 25°C for 45 min or at 0°C for 120 min prior to a direct plunge into liquid nitrogen. After rapid warming, the segments were expelled into Murashige and Skoog medium containing 1.2 M sucrose for 10 min and then plated on agar shoot outgrowth medium. The average rate of shoot formation of vitrified segments producing normal shoots was near 90% without any preculture and/or cold-acclimation treatment. Revived segments resumed growth within 3 days and developed about three shoots per segment. In vitro-cultured bud clusters appear promising as material for cryopreserving asparagus germplasm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00232685
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 4
    Digitale Medien
    Digitale Medien
    Cryopreservation of invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure (1997)
    Springer
    Plant cell reports 16 (1997), S. 594-599 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Shoot tips ; Cryopreservation ; Vitrification ; Taro [Colocasia esculenta (L.) Schott.] ; Tropical crops
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott.) were successfully cryopreserved by vitrification. Excised shoot tips precultured on solidified MS supplemented with 0.3M sucrose and maintained under a 16 h phtoperiod at 25°C for 16 h were loaded with a mixture of 2M glycerol plus 0.4M sucrose for 20 min at 25°C. The shoot tips were then sufficiently dehydrated with a highly concentrated vitrification solution (PVS2) for 20 min at 25°C prior to immersion into liquid nitrogen. Successfully vitrified and warmed shoot tips resumed growth within 7 days and developed shoots directly without intermediate callus formation. The average rate of shoot recovery amounted to around 80%, and the vitrification protocol appeared to be very promising for the cryopreservation of taro germplasm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01275498
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 5
    Digitale Medien
    Digitale Medien
    Amyloplast formation in cultured tobacco cells. III Determination of the timing of gene expression necessary for starch accumulation (1999)
    Springer
    Plant cell reports 18 (1999), S. 589-594 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key words ADP-glucose starch glycosyl transferase ; Amyloplast ; BY-2 ; Nicotiana tabacum ; Transcription/translation inhibitors
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract When BY-2 cultured tobacco (Nicotiana tabacum L.) cells were transferred to auxin-depleted culture medium containing cytokinin (benzyladenine, 1 mg/l), the starch content per cell started increasing from 18 h of culture and amyloplasts had formed by 48 h. Pulse-treatment of the cells with actinomycin D and cycloheximide for the first 12 h (or longer) of culture significantly decreased the cellular starch content after 48 h, whereas the starch content did not decrease significantly when the cells were released from the inhibition within 6 h. This suggests that nuclear gene expression necessary for amyloplast formation begins 6–12 h after the transfer. Immunoblotting analysis of the accumulation of ADP-glucose starch glycosyl transferase (starch synthase) supported this inference.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990050627
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 6
    Digitale Medien
    Digitale Medien
    Dispersion of the structural organization of proplastid-nuclei in vitro changes the transcriptional activity of plastid genes (1991)
    Springer
    Protoplasma 163 (1991), S. 203-206 
    Details
    ISSN: 1615-6102
    Schlagwort(e): DNA-protein interaction ; Nicotiana tabacum ; Proplastid ; Proplastid-nuclei (nucleoids) ; Transcription
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We have developed a novel assay system for analyzing the relationship between the structure and the transcriptional activity of the plastid-nuclei (plastid-nucleoids). The organization of morphologically intact proplastid-nuclei, isolated from tobacco cultured cells (line BY-2), was dispersed by treatment with NaCl at various concentrations and their transcriptional activities were examined by an assay of transcription in vitro and Southern hybridization. Disturbance of the structural organization of the proplastid-nuclei caused changes in both absolute and relative transcriptional activities of plastid genes, a result that suggests that the transcriptional activity of plastid genes may actually be regulated by structural changes in the plastid-nuclei.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01323345
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
Schließen ⊗
Diese Webseite nutzt Cookies und das Analyse-Tool Matomo. Weitere Informationen finden Sie hier...