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  • Aspergillus  (2)
  • Dimethylsulfoxide  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Characterization of the glyoxysomal isocitrate lyase genes of Aspergillus nidulans (acuD) and Neurospora crassa (acu-3) (1992)
    Springer
    Current genetics 21 (1992), S. 43-47 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Aspergillus ; Neurospora ; Isocitrate lyase ; Glyoxysome ; Acetate metabolism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The nucleotide sequences of the genes encoding the acetate-inducible glyoxylate cycle enzyme isocitrate lyase from the ascomycete fungi Aspergillus nidulans (acuD) and Neurospora crassa (acu-3) are presented. The respective A. nidulans and N. crassa genes are interrupted at identical positions by two introns and encode proteins of 538 and 543 amino acids, which have 75% identity. The predicted protein sequences do not demonstrate the C-terminal tripeptide S-K-L that has been implicated in peroxisomal targeting and found in the glyoxysomally located enzyme malate synthase from the same species. However, the protein sequences do exhibit a partial repeat which, in common with malate synthase, is located in regions that are absent from, or non-homologous with, the E. coli enzyme, which is not compartmentalized.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318653
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  • 2
    Digitale Medien
    Digitale Medien
    A thallium-205 NMR study of thallium acetate ion association in 2,2,2-trifluoroethanol and dimethylsulfoxide (1982)
    Springer
    Journal of solution chemistry 11 (1982), S. 379-386 
    Details
    ISSN: 1572-8927
    Schlagwort(e): Dimethylsulfoxide ; thallium acetate ; trifluoroethanol ; Tl-205 NMR chemical shifts
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract The association of thallium acetate ion in 2,2,2-trifluoroethanol and dimethyl-sulfoxide has been investigated by thallium-205 NMR spectroscopy. Analysis of the association constants as a function of temperature indicates that in trifluoroethanol the association enthalpy and entropy are, respectively, 1.6 kcal-mol−1 and 19.3 cal-mol−1-K−1 and in dimethylsulfoxide they are-0.99 kcal-mol−1 and 18.0 cal-mol−1-K−1. Examination of the temperature dependence of the chemical shift of the ion-pair reveals that in dimethylsulfoxide the ion-pair exists as a contact species, while in trifluoroethanol the solvent-separated ion-pair is more likely.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00649036
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  • 3
    Digitale Medien
    Digitale Medien
    Gene cloning in Aspergillus nidulans: isolation of the isocitrate lyase gene (acuD) (1986)
    Springer
    Molecular genetics and genomics 202 (1986), S. 271-275 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Aspergillus ; Isocitrate lyase ; Cloning ; Recombinant DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary An Aspergillus nidulans gene library was constructed in a high-frequency transformation vector, pDJB3, based on the Neurospora crassa pyr4 gene. This gene library was used to isolate the structural gene for isocitrate lyase (acuD) by complementation of a deficiency mutation following transformation of A. nidulans. Plasmids rescued in Escherichia coli were able to transform five different A. nidulans acuD mutants. Transformation using plasmids containing the cloned fragment resulted in integration at the acuD locus in six of nine transformants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00331649
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