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  • 1
    Electronic Resource
    Electronic Resource
    Protein targeting and secretion in filamentous fungi (1994)
    Springer
    Antonie van Leeuwenhoek 65 (1994), S. 211-216 
    Details
    ISSN: 1572-9699
    Keywords: Aspergillus niger ; filamentous fungi ; (conditional) secretion mutants ; reporter/carrier proteins ; subcellular compartments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Although the application of filamentous fungi, such asAspergillus niger for the production of extracellular proteins is well established for several decades, hardly any information is available about the molecular mechanisms of the process of protein secretion in these organisms. Two lines of research initiated towards a systematic analysis of the mechanism of protein targeting and secretion are presented in this paper. 1 — To study routing and targeting of proteins in filamentous fungi the availability of a versatile reporter/carrier protein will be of considerable importance. Experiments towards the identification of such a protein are presented. 2 — In analogy to the situation inSaccharomyces cerevisiae, the availability of defined (conditional) mutations in the secretion pathway will provide very important information about the organisation of the pathway. Therefore, based on results obtained inS. cerevisiae, the cloning of several fungal ‘secretion’ genes was started. The results of the cloning and characterisation of one of these genes is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00871949
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and analysis of functional homologues of the secretion-related SAR1 gene of Saccharomyces cerevisiae from Aspergillus niger and Trichoderma reesei (1997)
    Springer
    Molecular genetics and genomics 256 (1997), S. 446-455 
    Details
    ISSN: 1617-4623
    Keywords: Key words Gene cloning ; Protein secretion ; Filamentous fungi ; Small GTP binding protein ; Complementation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Aspergillusniger and Trichodermareesei genes encoding the functional homologues of the small GTP-binding protein SAR1p, which is involved in the secretion pathway in Saccharomyces cerevisiae, have been cloned and characterised. The A. niger gene (sarA) contains five introns, whereas the T. reesei gene (sar1) has only four. In both cases the first intron is at the same position as the single S. cerevisiae SAR1 intron. The encoded proteins show 70–80% identity to the SAR1 protein. Complementation of S. cerevisiaesar1 and sec12 mutants by expression vectors carrying the A. nigersarA and T. reesei sar1 cDNA clones confirmed that the cloned genes are functional homologues of the S. cerevisiae SAR1 gene. Three mutant alleles of the A. nigersarA gene (D29G, E109K, D29G/E109K), generated by site-directed mutagenesis, revealed a thermosensitive dominant-negative phenotype in the presence of the wild-type sarA allele. This result contrasts with the situation in S. cerevisiae, where similar mutations have a thermosensitive phenotype. Taken together, our results indicate that the sarA gene is involved in an essential function in A. niger.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008613
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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