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  • Associative learning  (1)
  • Biopsy  (1)
  • Flowcytometry  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Differences in performance on a reversal learning test and division of labor in honey bee colonies (2000)
    Springer
    Animal cognition 3 (2000), S. 119-125 
    Details
    ISSN: 1435-9456
    Keywords: Key words Proboscis extension reflex ; Associative learning ; Social insects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied the association between honey bee (Apis mellifera) division of labor and performance on an olfactory reversal-learning test. Manipulations of colony age structure and flight experience were used to test whether differences in performance are associated with age, current behavioral state, or flight experience. Nurse bees showed significantly faster rates of extinction to a learned odor than did foragers. This difference was associated primarily with differences in behavioral state, rather than age; it was seen when comparing nurses and foragers from typical colonies and normal-age nurses and precocious foragers from single-cohort colonies. Differences in extinction rate were not related to differences in flight experience; there was no difference between foragers and foraging-age bees denied flight experience. These results suggest that changes in learning and memory occur in association with division of labor. We speculate on the possible functional significance of the difference in extinction rate between nurses and foragers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100710000068
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Flowcytometric and cytogenetic analysis of human cultured cell lines derived from high- and low-grade astrocytomas (1983)
    Springer
    Acta neuropathologica 60 (1983), S. 40-48 
    Details
    ISSN: 1432-0533
    Keywords: Cytogenetics ; Flowcytometry ; T/E ratio ; Astrocytomas culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Five human cell lines cultured from high- and low-grade astrocytomas in cerebral hemisphere have been analyzed for DNA and protein distribution by flowcytometric (FCM) and correlated with cytogenetic profiles. Simultaneous calibration with chicken erythrocytes as a co-running standard provided an estimate of chromosomal number of predominate stem cells of each cell line by the ratio of the DNA content of the major peak (G1) to that of chicken erythrocyte (T/E ratio) of FCM. Various lines had different distributions of chromosomal number, ranging from near diploid to tetraploid. Each line had a stem-cell population and chromosomal markers indicative of clonal selection, but no common marker specific to astrocytomas. The histogram of DNA distribution obtained by FCM correlated well with the chromosomal distribution by cytogenetic analysis. In addition, simultaneous measurement of protein and DNA content in multidimensional FCM demonstrated a sigmoid configuration of the profiles, which indicated a gradual increase of protein content associated with an increase of chromosomal number or with progression of cell cycle. To avoid confusion of a bimodal chromosomal distribution with the G2/M phase of the cell cycle, and to determine chromosomal numbers associated with a DNA histogram, simultaneous cytogenetic and FCM study are required. More rapid than cytogenetic analysis, the T/E ratio allows estimation of chromosomal number of the stem-cell population associated with DNA histograms of cultured glioma-derived cell lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00685346
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Immunofluorescent staining of frozen sections for glial fibrillary acidic protein (1982)
    Springer
    Acta neuropathologica 58 (1982), S. 69-72 
    Details
    ISSN: 1432-0533
    Keywords: GFAP ; Glioma ; Biopsy ; Astrocytoma ; CNS tumor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Immunofluorescent staining for glial fibrillary acidic protein (GFAP) has been used as part of the diagnostic evaluation of eleven patients and compared with routine special stains. In one case, a difficult fibrillary neoplasm of the spinal cord, this diagnostic procedure provided rapid, positive identification of the glial nature of the tumor. In all cases, the GFAP reactivity was consistent with staining properties of PTAH and more rapid than PTAH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00692700
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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