ISSN:
0021-9304
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
,
Technology
Notes:
A series of studies were performed using S35-labeled heparin to determine the amount and the permanancy of heparin grafted onto nonthrombogenic surfaces. The calculated thicknesses of the heparin layers (based on geometrical areas) ranged from 100 to 1500 A. It was shown that the surface having a 100-A layer of attached heparin was nonthrombogenic. The stability of the heparin attached to polypropylene, silicone rubber, hydrin rubber, and graphite-benzalkonium-heparin (GBH) surfaces in distilled water, isotonic saline, and human plasma was measured. The results showed that after exposure to water and saline, the first three surfaces retained most of the attached heparin (〉90%) in contrast to the GBH surface which retained only 31%. After contact with human plasma for 3 hr at 37°C, polypropylene retained 32% and GBH surfaces 19% of the heparin which was initially present. In another series of experiments, the adsorption of P32-labeled proteins onto unheparinized, quaternary ammonium, and heparinized surfaces was measured. The results indicated that the amounts of albumin, Hageman factor, thrombin, and γ-globulin adsorbed onto heparinized surfaces were equal to or greater than the amounts adsorbed onto unheparinized surfaces. The implications of this in terms of the nonthrombogenicity of the heparinized surfaces are discussed.
Additional Material:
7 Tab.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jbm.820010205
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