Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Atopic dermatitis  (4)
  • Chemiluminescence  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Modulation of granulocyte oxidative response by recombinant interferon α2 and γ (1986)
    Springer
    Archives of dermatological research 278 (1986), S. 274-276 
    Details
    ISSN: 1432-069X
    Keywords: Interferon α2 ; Interferon γ ; Granulocytes ; Chemiluminescence ; Lucigenin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Interferon (IFN) has been described to influence various cellular functions. In this study we investigated whether the oxidative response of polymorphonuclear leukocytes (PMN) is also affected by IFN. In order to exclude the possible influence of impurities in IFN preparations, only recombinant human IFN α2 or γ were used. Lucigenin-dependent chemiluminescence (CL) of PMN was measured to assess the production of oxygen radicals. IFN γ at a concentration of more than 10 ng/ml elicited a minimal CL response in PMN. When PMN were incubated with IFN γ for 1 h and then stimulated with chemotactic peptide f-met-phe (FMP), zymosan-activated serum (ZAS), zymosan particles, or phorbol-myristate acetate (PMA), the CL response was increased as consequence of the generally enhanced oxidative metabolism. IFN α2 showed no such effect at any concentration tested. A 5-min pretreatment with IFN γ decreased the ZAS response but did not affect the reaction to the other stimuli. The possibility of a generation of IFN by PMN during the assay could be excluded as no IFN activity could be detected in an antiviral assay after stimulation of PMN for 6 h with PolyIxPolyC, LPS, ConA, C. parvum, PMA, zymosan, or FMP. The modulation of granulocyte activity by IFN γ may be important in the regulation of the anti-inflammatory response of PMN.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407737
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Inhibitory effect of sulfonated shale oils (ammonium bituminosulfonate) on the stimulation of neutrophilic granulocytes by the chemotactic tripeptide f-Met-Leu-Phe (1986)
    Springer
    Archives of dermatological research 278 (1986), S. 190-193 
    Details
    ISSN: 1432-069X
    Keywords: Adherence ; Bituminosulfonate ; Chemiluminescence ; Chemotactic factor ; Enzyme release ; Neutrophils ; Superoxide anion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Sulfonated shale oils (ammonium bituminosulfonate, ichthammol, Ichthyol), shown previously to induce the directed migration of human neutrophils in Boyden chambers and to inhibit the directed migration towards the chemotactic factors C5a, LTB4, and f-Met-Leu-Phe, were studied for their effect on other neutrophil functions, which are stimulated by chemotactic factors. Like other chemotactic factors ammonium bituminosulfonate increased the adherence of neutrophils to nylon fibers, but it did not induce the release of the primary granule enzyme glucosaminidase from cytochalasin B-treated cells and it did not stimulate the production of oxygen radicals as measured by lucigenin-dependent chemiluminescence if studied under nontoxic conditions. When added together with the chemotactic tripeptide f-Met-Leu-Phe, ammonium bituminosulfonate inhibited adherence augmentation, enzyme release, and oxygen-radical production induced by the chemotactic factor. The results indicate that ammonium bituminosulfonate not only inhibited chemotactic migration but the whole spectrum of neutrophil functions induced by a chemotactic factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412922
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Detection of complement activation in human serum using C5S-Induced chemiluminescence of human granulocytes (1985)
    Springer
    Archives of dermatological research 278 (1985), S. 41-43 
    Details
    ISSN: 1432-069X
    Keywords: Complement ; Anaphylatoxins ; C5a ; Chemiluminescence ; Granulocytes ; Lucigenin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The complement system can be activated by many factors, including immune complexes, leading to the generation of biologically active split products like C5a anaphylatoxin. This study presents a technique which may be used for measuring C5a activity in human serum. The tetanus-anti-tetanus immune complex and aggregated human IgG were used as model activators of the complement cascade. The C5a activity was measured by C5a-induced chemiluminescence of granulocytes; furthermore, a radioimmunoassay was used to detect the C5a peptide. There was a strongly positive correlation between the two assay systems. The described method should be useful as an alternative means of detecting complement activation in the serum of patients with inflammatory diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412494
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    T-Cell function in atopic dermatitis controlled examination of Concanavalin A dose-response relations in cultured lymphocytes (1978)
    Springer
    Archives of dermatological research 262 (1978), S. 37-44 
    Details
    ISSN: 1432-069X
    Keywords: Atopic dermatitis ; Cell-mediated immunity ; T-lymphocytes ; Concanavalin A ; Neurodermitis atopica ; Zellvermittelte Immunität ; T-Lymphozyten ; Concanavalin A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei Patienten mit Neurodermitis atopica leichten Schweregrades konnte im Rahmen einer kontrollierten Studie mit Hilfe von “matched pairs” eine gegenüber gesunden Kontrollpersonen signifikant (α=0,005) herabgesetzte in vitro 3H-Thymidin-Einbaurate in kultivierte Lymphozyten des peripheren Blutes, nach Stimulation mit dem T-Zellenmitogen Concanavalin A (Con A), festgestellt werden. Anhand von zehn differenten Konzentrationen von Con A wurden die Dosis-Wirkungsbeziehungen innerhalb der Paare bestimmt. Während höhere Con A-Konzentrationen in den Lymphozytenkulturen zu optimalen 3H-Thymidin-Einbauraten in neusynthetisierte DNS führten, waren die Unterschiede zwischen Neurodermitikern und Kontrollpersonen eindeutiger bei niedrigeren, suboptimalen Con A-Konzentrationen. Die spontane 3H-Thymidin-Einbaurate in kultivierte Lymphozyten ohne Con A-Stimulation (spontane DNS-Synthese) war bei Neurodermitikern im Vergleich zu Kontrollpersonen erhöht. Die Befunde weisen auf eine beeinträchtigte T-Zellenfunktion bei Neurodermitikern hin.
    Notes: Summary The Concanavalin A (Con A) stimulation response of peripheral lymphocytes in vitro was found to be decreased statistically significant (α=0.005) in patients with light atopic dermatitis, Strictly matched pairs were examined. Using ten graded concentrations of Con A, dose-response relations of the pairs were demonstrated, differences were more evident at lower suboptimal concentrations. Background DNA-synthesis of the patients was elevated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455571
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Involvement of complement in psoriasis and atopic dermatitis — Measurement of C3a and C5a, C3, C4 and C1 inactivator (1985)
    Springer
    Archives of dermatological research 277 (1985), S. 359-361 
    Details
    ISSN: 1432-069X
    Keywords: Complement ; Anaphylatoxins ; Psoriasis ; Atopic dermatitis ; C3a ; C5a
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Normal complement components and activation products were determined in the peripheral blood of 35 patients with atopic dermatitis (AD) and 24 patients with psoriasis at a mild to intermediate stage. None of the patients had received systemic or local steroid therapy 6 weeks prior to blood collection. Levels of C3, C4 and C1 inactivator (C1 INA) were determined in serum by radial immunodiffusion, whereas C3a and C5a levels were measured by radioimmunoassay. In comparison to healthy non-atopic controls, the levels of C3, C4 and C1 INA were found to be significantly increased in both diseases. No substantial differences were detected between patients with psoriasis vulgaris and psoriasis guttata, which suggests that the dissimilarities found were not due to preceding or concomitant infections. In AD, there was a tendency towards increased C3a levels, whereas in psoriasis, C3a levels were significantly increased. In both diseases, no measurable amounts of C5a could be detected. The results indicate that, in both AD and psoriasis, the complement participates in the inflammatory process. Elevated levels of C3a suggest that there is a continuous activation of the complement system leading to the generation of inflammatory mediators.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00509233
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Production of interferon and lymphoproliferative response in whole blood cultures derived from patients with atopic dermatitis (1987)
    Springer
    Archives of dermatological research 279 (1987), S. S55 
    Details
    ISSN: 1432-069X
    Keywords: Interferon ; Lymphoproliferative response ; Atopic dermatitis ; Whole blood cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Enhanced susceptibility to viral infections has been reported repeatedly in atopic dermatitis (AD). A difference in the capacity to produce interferons (IFN) in response to viral antigens may be the cause. In the present study we investigated the in vitro IFN production of leukocytes from AD patients in response to different stimuli. Furthermore, the lymphoproliferative responses were tested. The patients showed moderate to severe diesease activity. Whole blood cultures of 25 AD patients and 21 healthy nonatopic controls were stimulated with the mitogens phytohemagglutinin (PHA), concanavalin A (ConA), and pokeweed mitogen (PWM), with tuberculin derivative (PPD) and tetanus antigen as IFN-γinducers, and withC. parvum, poly I-poly C, and herpes simplex virus (HSV) as inducers of IFN-a Lymphoproliferation was assayed in 5-day cultures in parallel. In AD no significant difference of the IFN production was found in comparison with the controls with any stimuli tested. The lymphoproliferative response of leukocytes of patients with AD was significantly decrased upon stimulation with PHA, Con A, PWM, and PPD. We suggest that in AD the described susceptibility to viral infections is not due to an altered capability of leukocytes to generate IFN.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00585921
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Elevated levels of soluble CD8 antigen in sera of patients with psoriasis — a possible sign of suppressor/cytotoxic T-cell activation (1990)
    Springer
    Archives of dermatological research 282 (1990), S. 6-7 
    Details
    ISSN: 1432-069X
    Keywords: Psoriasis ; T-cell activation ; CD8 antigen ; Psoriatic arthritis ; Atopic dermatitis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Increased levels of the soluble CD8 antigen probably represent a sign of suppressor T-cell activity. The concentration of CD8 was measured in the sera of patients with psoriasis without psoriatic arthritis (n=25) and atopic dermatitis (n=26), exhibiting moderate to severe disease activity, in comparison with nonatopic healthy controls (n=31) using an ELISA technique. CD8 levels were found to be significantly elevated in psoriatic patients as compared with healthy controls (p≤0.002). Increase in CD8 may be due to suppressor T-cell activation in the skin of patients with psoriasis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00505637
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Effect of tumour necrosis factor alpha in vivo on human granulocyte oxidative metabolism (1991)
    Springer
    Archives of dermatological research 283 (1991), S. 362-365 
    Details
    ISSN: 1432-069X
    Keywords: TNFα ; PMN ; Oxidative metabolism ; Chemiluminescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Tumour necrosis factor alpha (TNFα) effectively stimulates the oxidative metabolism of human PMN in vitro. Moreover, preincubation of PMN with TNFα has been shown to result in an altered response of the target cells to subsequent stimulation. In the present study the response of PMN to stimulation in vitro was investigated in patients with metastasizing malignant melanoma receiving bolus injections of recombinant human TNFα as therapy. TNFα was given daily for 5 days. Blood samples were taken prior to TNFα administration on days 1 to 4 and on day 8. Lucigenin-enhanced chemiluminescence (CL) was used as a sensitive measure of granulocyte oxidative metabolism. PMN were stimulated with TNFα, TNFΒ, GM-CSF, PMA, opsonized zymosan and f-met-leu-phe. A significant increase in CL responses was detected upon stimulation with TNFα, TNFΒ and PMA from day 1 to day 3, whereas no significant changes were observed for the background activity or when GM-CSF or opsonized zymosan were used as stimuli. On day 4 all CL responses returned to the day 1 starting level. A further significant decrease was observed on day 8 upon stimulation with TNFα, TNFΒ and GM-CSF. In contrast, the effect induced by f-met-leu-phe reached a maximum on day 4, but the CL response was found to be at the starting level on day 8. The results indicate that TNFα induces significant changes in PMN response to distinct stimuli in vivo. Moreover, it may be possible that continous daily infusions with TNFα induce a hyposensitization of PMN oxidative metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00371816
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...