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  • Somatic hybridization  (2)
  • Avena  (1)
  • B. oleracea  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Studies on the fungal phytotoxin victorin: Structures of three novel amino acids from the acid hydrolyzate (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1370-1374 
    Details
    ISSN: 1420-9071
    Keywords: Cochliobolus victoriae ; Helminthosporium ; Bipolaris ; Avena
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The host-selective phytotoxin victorin, produced by the fungusCochliobolus victoriae, was found to be at least partially peptidic in nature, and did not contain victoxinine. The exact mass of the M-H ion was measured by FABMS as 795.1877. Derivatives of three major acid hydrolysis products were isolated. The structures of the corresponding amino acids were assigned as 2S,3R-3-hydroxyleucine, 5,5-dichloroleucine, and 3-hydroxylysine. A into victorin by the fungus in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01949994
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Improved resistance to bacterial soft rot by protoplast fusion between Brassica rapa and B. oleracea (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 810-819 
    Details
    ISSN: 1432-2242
    Keywords: Key words Bacterial soft rot ; Disease resistance ; Chinese cabbage ; Protoplast fusion ; Brassica rapa ; B. oleracea ; B. napus ; Erwinia carotovora subsp. carotovora
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Erwinia soft rot is a destructive disease of Brassica rapa vegetables. Reliable sources of resistance and control methods are limited, so development of highly resistant breeding lines is desirable. Protoplasts from B. rapa and B. oleracea genotypes selected for resistance to soft rot were fused in order to combine different sources of resistance. Twelve somatic hybrids (synthetic B. napus) were obtained and confirmed by morphology, nuclear DNA content, and RAPD analysis. They were normal looking plants that easily set seeds following self-pollination and backcrossing to B. rapa. Assays of detached leaves or seedlings inoculated in a mist-chamber showed that most somatic hybrids had lower disease severity ratings than the B. rapa fusion partner and a commercial variety of B. napus. Some progeny from selfing or backcrossing of somatic hybrids to B. rapa showed much more resistance than either fusion partner. The offspring populations of the somatic hybrids (F1–S1 and F1–BC1) clearly moved to the resistant direction compared to the parents; the percentage of resistant plants increased from 21% (average of parents) to 36% (F1–S1) and 48% (F1–BC1). These results suggest that it may be possible to obtain highly resistant B. rapa lines by further backcrossing and selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051356
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of organelle genomes in a somatic hybrid derived from cytoplasmic male-sterile Brassica oleracea and atrazine-resistant B. campestris (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 303-309 
    Details
    ISSN: 1432-2242
    Keywords: Somatic hybridization ; Atrazine resistance ; Mitochondrial recombinants ; Brassica ; Cytoplasmic male sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An atrazine-resistant, male-fertile Brassica napus plant was synthesized by fusion of protoplasts from the diploid species B. oleracea and B. campestris. Leaf protoplasts from B. oleracea var. italica carrying the Ogura male-sterile cytoplasm derived from Raphanus sativus were fused with etiolated hypocotyl protoplasts of atrazine-resistant B. campestris. The selection procedure was based on the inability of B. campestris protoplasts to regenerate in the media used, and the reduction of light-induced growth of B. oleracea tissue by atrazine. A somatic hybrid plant that differed in morphology from both B. oleracea and B. campestris was regenerated on medium containing 50 μM atrazine. Its chromosome number was 36–38, approximately that of B. napus. Furthermore, nuclear ribosomal DNA from this hybrid was a mixture of both parental rDNAs. Southern blot analyses of chloroplast DNA and an assay involving tetrazolium blue indicated that the hybrid contained atrazine-resistant B. campestris chloroplasts. The hybrid's mitochondrial genome was recombinant, containing fragments unique to each parent, as well as novel fragments carrying putative crossover points. Although the plant was female-sterile, it was successfully used to pollinate B. napus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274711
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Transfer of resistance to Xanthomonas campestris pv campestris into Brassica oleracea L. by protoplast fusion (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 1293-1300 
    Details
    ISSN: 1432-2242
    Keywords: Somatic hybridization ; Black rot ; Disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Black rot caused by the bacterium Xanthomonas campestris pv campestris is one of the most serious diseases of Brassica oleracea. Since sources of resistance to the disease within B. oleracea are insufficient and control means are limited, the development of resistant breeding lines is extremely desirable. Certain lines of B. napus contain very high resistance controlled by a dominant gene, but crossing the two species sexually is very difficult. Therefore, somatic hybrids were produced by protoplast fusion between rapid cycling B. oleracea and a B. napus line highly resistant to X. campestris pv campestris. Hybrid identity was confirmed by morphological studies, flow cytometric estimation of nuclear DNA content, and analysis of random amplified polymorphic DNA (RAPD). Inoculations with the pathogen identified four somatic hybrids with high resistance. The resistant hybrid plants were fertile and set seed when selfed or crossed reciprocally to the bridge line ‘15’ (Quazi 1988). Direct crosses to B. oleracea were unsuccessful, but embryo rescue facilitated the production of a first-backcross generation. The BC1 plants were resistant to the pathogen. Progeny from the crosses to ‘line 15’ were all susceptible. Embryo rescue techniques were not obligatory for the development of a second-backcross generation, and several resistant BC2 plants were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220944
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    Paper (German National Licenses)
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