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  • Brassica napus  (2)
  • Reversion to fertility  (2)
  • Avena  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Studies on the fungal phytotoxin victorin: Structures of three novel amino acids from the acid hydrolyzate (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1370-1374 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Cochliobolus victoriae ; Helminthosporium ; Bipolaris ; Avena
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The host-selective phytotoxin victorin, produced by the fungusCochliobolus victoriae, was found to be at least partially peptidic in nature, and did not contain victoxinine. The exact mass of the M-H ion was measured by FABMS as 795.1877. Derivatives of three major acid hydrolysis products were isolated. The structures of the corresponding amino acids were assigned as 2S,3R-3-hydroxyleucine, 5,5-dichloroleucine, and 3-hydroxylysine. A into victorin by the fungus in vivo.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01949994
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  • 2
    Digitale Medien
    Digitale Medien
    Synthesis of male sterile, triazine-resistant Brassica napus by somatic hybridization between cytoplasmic male sterile B. oleracea and atrazine-resistant B. campestris (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 445-455 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Brassica napus ; Protoplast fusion ; Triazine resistance ; Cytoplasmic male sterility ; Mitochondrial DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Fusion of leaf protoplasts from an inbred line of Brassica oleracea ssp. botrytis (cauliflower, n=9) carrying the Ogura (R1) male sterile cytoplasm with hypocotyl protoplasts of B. campestris ssp. oleifera (cv “Candle”, n=10) carrying an atrazine-resistant (ATR) cytoplasm resulted in the production of synthetic B. napus (n=19). Thirty-four somatic hybrids were produced; they were characterized for morphology, phosphoglucose isomerase isoenzymes, ribosomal DNA hybridization patterns, chromosome numbers, and organelle composition. All somatic hybrids carried atrazine-resistant chloroplasts derived from B. campestris. The mitochondrial genomes in 19 hybrids were examined by restriction endonuclease and Southern blot analyses. Twelve of the 19 hybrids contained mitochondria showing novel DNA restriction patterns; of these 12 hybrids, 5 were male sterile and 7 were male fertile. The remaining hybrids contained mitochondrial DNA that was identical to that of the ATR parent and all were male fertile.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00265310
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  • 3
    Digitale Medien
    Digitale Medien
    Fertile revertants from S-type male-sterile maize grown in vitro (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 601-609 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Cytoplasmic male-sterility (cms) ; Reversion to fertility ; Mitochondrial DNA ; Maize ; Somaclonal variation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Plants were regenerated from callus cultures of maize inbred W182BN with the S(USDA) type of cytoplasmic male sterility (cms). Some regenerates from 16 of 18 separate cultures had fertile tassels. Many other regenerates, whose fertility could not be scored accurately because of abnormal plant morphology, produced fertile progeny after pollination with N cytoplasm W182BN. Revertant plants and/or progeny were obtained from all 18 cultures, which included the CA, D, LBN, and S sources of cmsS. More revertants were recovered from cultures maintained as callus for 12 months than from 3–4 month old cultures. Several types of evidence (absence of segregation for fertility after selfing or pollination of revertants with standard W182BN, pollen viability counts, failure of revertants to restore sterile cmsS lines to fertility, mitochondrial DNA analyses) indicated that the reversion to fertility involved cytoplasmic rather than nuclear alterations. All revertants examined lacked the S1 and S2 plasmid-like DNAs characteristic of the mitochondrial genome of sterile cmsS lines. Most callus cultures lost S1 and S2 after 13–20 months in vitro. No revertants were seen among thousands of W182BN cmsS plants grown from seed in the field or among plants from tissue cultures of W182BN with the C or T types of cms. The cytoplasmic revertants recovered from culture may be useful for the molecular analysis of cmsS.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00288859
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  • 4
    Digitale Medien
    Digitale Medien
    Synthesis of high erucic acid rapeseed (Brassica napus L.) somatic hybrids with improved agronomic characters (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 1129-1136 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Brassica napus ; somatic hybrids ; protoplast fusion ; Erucic acid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Novel Brassica napus somatic hybrids have been created through protoplast fusion of B. oleracea var. botrytis and B. rapa var. oleifera genotypes selected for high erucic acid (22:1) content in the seed oil. Fifty amphidiploids (aacc) and one putative hexaploid (aacccc) hybrid were recovered in one fusion experiment. Conversely, only one amphidiploid and numerous regenerates with higher DNA contents were produced in a similar fusion using a different B. rapa partner. Hybridity was confirmed by morphology, isozyme expression, flow cytometry, and DNA hybridization. Analysis of organellar DNA revealed a distinct bias toward the inheritance of chloroplasts from the B. rapa (aa) genome. All amphidiploids set self-pollinated seed. A erucic acid content as high as 57.4% was found in the seed oil of one regenerated plant. Fatty acid composition was stable in the R1 generation and was coupled with increased female fertility. Other novel agronomic characters in the hybrids recovered include large seed size, lodging resistance, and non-shattering seed pods.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223931
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  • 5
    Digitale Medien
    Digitale Medien
    A comparison of cytoplasmic revertants to fertility from different CMS-S maize sources (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 609-618 
    Details
    ISSN: 1432-2242
    Schlagwort(e): S-type cytoplasmic male sterility ; Reversion to fertility ; Mitochondrial genome organization ; Recombination ; Maize
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The mitochondrial genome organizations of a number of independent culture-derived fertile CMS-S revertants with the nuclear genotype W182BN were compared to spontaneous field revertants with the genotypes WF9, M825/Oh07 and 38-11. Regions of the genome around sequences homologous to the terminal repeats of the linear S1 and S2 episomes characteristic of CMS-S mitochondria were used as hybridization probes on Southern blots of BamHI and SalI digested mitochondrial DNA. The results obtained suggest that the nuclear, not the cytoplasmic, genotype of the parent plant affects the type of novel mitochondrial DNA organization found in the revertant. The DNA reorganization during reversion from CMS-S in tissue culture appears to be similar to that observed in spontaneous revertants obtained during the normal plant life-cycle. Unlike the situation for reversion from CMS-T, no common DNA sequence or reading frame appeared to be lost or disrupted in revertants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00260916
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