ISSN:
1432-0533
Keywords:
Brain
;
Silver staining
;
Microwave technique
;
Axon terminal
;
Histology
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary A simple and rapid method for light and electron microscopic visualization of degenerating neuronal processes and axon terminals is described. Hundred-micrometer vibratome sections of perfusion-fixed rat brain were incubated briefly in a 5% silver nitrate solution in a conventional microwave oven. After a rinse in 1% acetic acid, the sections were silver enhanced. Differentiation and counterstaining was done respectively in ethanol 100% and cresyl violet. In the light microscope, degenerating neuronal processes appeared as black dots against a clear background. Areas of calcification were also positively stained. The presence of silver deposits in degenerating presynaptic terminals and dendrites was confirmed ultrastructurally.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00311395
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