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  • Chemistry  (6)
  • Column liquid chromatography  (4)
  • B chain of oxidized insulin  (2)
  • Collagen  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Proteolytic activity and cleavage specificity of cathepsin E at the physiological pH as examined towards the B chain of oxidized insulin
    Amsterdam : Elsevier
    FEBS Letters 292 (1991), S. 53-56 
    Details
    ISSN: 0014-5793
    Keywords: B chain of oxidized insulin ; Cathepsin E ; Cleavage specificity ; Gastric mucosal aspartic proteinase ; Nph ; PAGE ; Proteolytic activity ; SDS ; cya ; cysteic acid ; nitrophenylalanine ; polyacrylamide gel electrophoresis ; sodium dodecyl sulfate
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-5793(91)80832-N
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Proteolytic activity and cleavage specificity of cathepsin E at the physiological pH as examined towards the B chain of oxidized insulin
    Amsterdam : Elsevier
    FEBS Letters 292 (1991), S. 53-56 
    Details
    ISSN: 0014-5793
    Keywords: B chain of oxidized insulin ; Cathepsin E ; Cleavage specificity ; Gastric mucosal aspartic proteinase ; Proteolytic activity
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-5793(91)80832-N
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The complete primary structure of the long form of mouse α1(IX) collagen chain and its expression during limb development
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular 1204 (1994), S. 61-67 
    Details
    ISSN: 0167-4838
    Keywords: (Mouse) ; Cartilage ; Chondrocyte ; Collagen ; Endochondral ossification ; Gene expression ; Limb development
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4838(94)90033-7
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  • 4
    Electronic Resource
    Electronic Resource
    Alginate oligosaccharides modulate cell morphology, cell proliferation and collagen expression in human skin fibroblasts in vitro (1999)
    Springer
    Archives of dermatological research 291 (1999), S. 432-436 
    Details
    ISSN: 1432-069X
    Keywords: Key words Alginate oligosaccharides ; Collagen ; Human skin fibroblasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Effects of alginate oligosaccharides on cell proliferation and expression of collagen in cultured skin fibroblasts were studied. The oligosaccharides were found to suppress fibroblast proliferation to half the level in control cultures at a dose of 10 mg/ml during a period of 5 days. The inhibition was accompanied by a change in cell shape. The inhibition of cell proliferation was reversible, since depletion of these oligosaccharides led to a recovery of cell motility. Treatment of confluent cells with 10 mg/ml oligosaccharides for 5 days resulted in a reduction in collagen synthesis to one half of that in control cultures and inhibition of steady state levels of α1(I), α2(I), α1(III) and α1(VI) collagen mRNAs. These results suggest that alginate oligosaccharides are potential modulators of dermal fibroblasts and may provide a useful tool for the treatment of disorders related to abnormal collagen metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004030050434
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  • 5
    Electronic Resource
    Electronic Resource
    Determination of pheophytinatonickel(II) by reversed-phase highperformance liquid chromatography (1988)
    Springer
    Chromatographia 25 (1988), S. 319-323 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Pheophytinatonicke(II) ; Chlorophyll ; Pheophytin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary HPLC determination of pheophytinatonicke(II) (Pheo-Ni) prepared by the replacement of magnesium(II) in chlorophyll with nicke(II) is described. The good separation of PheoNi was obtained by using chemically bonded C18 as the stationary phase and acetone-methanol (50∶50, vol/vol) as the mobile phase. Conventional spectrophotometric method was also used for the determination of PheoNi. For the synthetic samples prepared by mixing (pheophytinato a) nicke(II) [(Pheo-a) Ni] and (pheophytinato b) nicke(II) [(Pheo-b) Ni], analytical values obtained by the spectrophotometric method were very high compared to those obtained by HPLC. In the proposed HPLC method, (Pheo-a) Ni and (Pheo-b). Ni could be determined in the concentration range of 0.028–30μg/ml and 0.038–30μg/ml with relative standard deviations (n=10) of 3.1% and 0.8%, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02324707
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  • 6
    Electronic Resource
    Electronic Resource
    Preparation and determination of manganese(III) chlorophylls by reversed-phase high performance liquid chromatography (1992)
    Springer
    Chromatographia 33 (1992), S. 567-570 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chlorophylls ; Manganese(III) chlorophylls
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Manganese chlorophylls have been synthesized by refluxing a mixture of pheophytins dissolved in acetone and manganese(II) acetate anhydride dissolved in glacial acetic acid. A good separation of manganese(III) chlorophylls has been attained by RP-HPLC using chemically bonded C18 silica as a stationary phase and methanol with 3% acetic acid as a mobile phase. An accurate and rapid HPLC method is described for the simultaneous determination of manganese(III) chlorophyll-a [Mn(III)-chl-a] and manganese(III) chlorophyll-b [Mn(III)-chl-b]. Tailing arising from dissociation of acetate ions is improved by addition of sodium acetate (5×10−3 M) to the mobile phase (acetone: methanol=90∶10, vol/vol). The analytical values obtained by the HPLC method are very close to the calculated contents in all samples, but those obtained by spectrophotometry are high because of the interferences from overlapping of absorption bands. In the proposed HPLC method the calibration graphs of Mn(III)-chl-a and Mn(III)-chl-b are linear in the concentration range 0–20 μg cm−3 with relative standard deviations (n=10) of 3.46% and 4.54%, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02262249
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  • 7
    Electronic Resource
    Electronic Resource
    Preparation and separation of metallobacteriochlorophylla by reversed-phase high-performance liquid chromatography (1996)
    Springer
    Chromatographia 43 (1996), S. 361-364 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Bacteriochlorophylla ; Copper(II) bacteriochlorophylla ; Zinc(II) bacteriochlorophylla
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Semi-preparative high-performance liquid chromatography has been used for the preparation of copper(II) bacteriochlorophylla [Cu(II)-BChl-a] and zinc(II) bacteriochlorophylla [Zn(II)-BChl-a]. Both compounds are separated on a reversed-phase Inertsil ODS-2 column using a mobile phase of acetone-methanol (25∶75, v/v). The fractionated metallobacteriochlorophylls (M-Bchl-a) are identified by fast atom bombardment mass spectrometry. The spectroscopic parameters such as the wavelength of absorption maxima and the molar extinction coefficients are determined using pure M-Bchl-a obtained by preparative HPLC. The HPLC method proposed here has been demonstrated to be useful for the purification and determination of components of M-Bchl-a.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02271011
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  • 8
    Electronic Resource
    Electronic Resource
    Transport of a gas through asymmetric polysulfone membranes with deposited plasma-polymerized thin layer (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 41 (1990), S. 2427-2436 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A plasma-polymerized thin layer was deposited on the top surface of the skin layer of the asymmetric polysulfone (PSF) membrane to plug ultrafine pores passing through it. Trifluoromethane was used as the monomer gas of plasma polymerization. The steady-state permeation rates for oxygen and nitrogen through the asymmetric PSF membranes with the plasma modification were measured to evaluate the permselectivity and permeability. If ultrafine pores passing through the asymmetric membrane can be plugged by the plasma-polymerized thin layer, the separation factor of oxygen relative to nitrogen can be increased with the permeation rate coefficient of oxygen still kept large. This condition was met when the vaporization time of the cast solution during the preparation of an asymmetric membrane ranged from 10 to 120 s and the plasma polymerization time ranged from 15 to 30 min.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1990.070410942
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of organic solvents on the activity of glucoamylase (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 28 (1986), S. 1172-1177 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Hydrolyses of maltose, maltotriose, and soluble starch catalyzed by glucoamylase (Asp. Niger) were carried out in the aqueous solutions of methanol, ethanol, ethylene glycol, and 1,4-dioxane at 40°C and at the optimum pH in the respective solutions. By the kinetic analysis based on the subsite model, it was shown that the intrinsic rate constant, Kint, was electrostatically affected by the dielectric constant of the hydroorganic solutions. The affinity of the third subsite, A3, which affects the apparent rate constant, K0, was correlated with the ×Gtr's of maltose and amino acid side chains.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260280807
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  • 10
    Electronic Resource
    Electronic Resource
    Viscosity of concentrated styrene solutions of polystyrene (1968)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 12 (1968), S. 9-11 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Viscosities of concentrated solutions of polystyrene of different intrinsic viscosities were measured at varying polymer concentration and temperature. By modifying Martin's equation, an empirical equation correlating the viscosity of the solution as a function of the polymer concentration, the intrinsic viscosity of the polymer, and temperature was obtained.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1968.070120102
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